Determination of Chinese hamster ovary (CHO) cell densities and antibody titers from small volumes of cell culture supernatants using multivariate analysis and partial least squares regression of UV-Vis spectra

Antibody titer and viable cell density (VCD) are two important parameters that need to be closely monitored during the process of cell line development and manufacturing of therapeutic antibodies. Typically, determination of each parameter requires 10–100 μL of supernatant sample, which is not suita...

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Detalles Bibliográficos
Autores principales: Jarusintanakorn, Salinthip, Phechkrajang, Chutima, Khongkaew, Putthiporn, Mastrobattista, Enrico, Yamabhai, Montarop
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8437849/
https://www.ncbi.nlm.nih.gov/pubmed/34476523
http://dx.doi.org/10.1007/s00216-021-03549-4

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