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Time-resolved in vivo ubiquitinome profiling by DIA-MS reveals USP7 targets on a proteome-wide scale

Mass spectrometry (MS)-based ubiquitinomics provides system-level understanding of ubiquitin signaling. Here we present a scalable workflow for deep and precise in vivo ubiquitinome profiling, coupling an improved sample preparation protocol with data-independent acquisition (DIA)-MS and neural netw...

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Detalles Bibliográficos
Autores principales:	Steger, Martin, Demichev, Vadim, Backman, Mattias, Ohmayer, Uli, Ihmor, Phillip, Müller, Stefan, Ralser, Markus, Daub, Henrik
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438043/ https://www.ncbi.nlm.nih.gov/pubmed/34518535 http://dx.doi.org/10.1038/s41467-021-25454-1

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