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Comprehensive Analysis of circRNA Expression Profiles During Cervical Carcinogenesis

Circular RNAs (circRNAs) are regulatory molecules that participate in the occurrence, development and progression of tumors. To obtain a complete blueprint of cervical carcinogenesis, we analyzed the temporal transcriptomic landscapes of mRNAs and circRNAs. Microarrays were performed to identify the...

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Autores principales: Luo, Haixia, Li, Yuanxing, Zhao, Yueyang, Chang, Jingjing, Zhang, Xiu, Zou, Binbin, Gao, Lifang, Wang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438239/
https://www.ncbi.nlm.nih.gov/pubmed/34532284
http://dx.doi.org/10.3389/fonc.2021.676609
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author Luo, Haixia
Li, Yuanxing
Zhao, Yueyang
Chang, Jingjing
Zhang, Xiu
Zou, Binbin
Gao, Lifang
Wang, Wei
author_facet Luo, Haixia
Li, Yuanxing
Zhao, Yueyang
Chang, Jingjing
Zhang, Xiu
Zou, Binbin
Gao, Lifang
Wang, Wei
author_sort Luo, Haixia
collection PubMed
description Circular RNAs (circRNAs) are regulatory molecules that participate in the occurrence, development and progression of tumors. To obtain a complete blueprint of cervical carcinogenesis, we analyzed the temporal transcriptomic landscapes of mRNAs and circRNAs. Microarrays were performed to identify the circRNA and mRNA expression profiles of cervical squamous cell carcinoma (CSCC) and high-grade squamous intraepithelial lesion (HSIL) patients compared with normal controls (NC). Short time-series expression miner (STEM) was utilized to characterize the time-course expression patterns of circRNAs and mRNAs from NC to HSIL and CSCC. A total of 3 circRNA profiles and 3 mRNA profiles with continuous upregulated patterns were identified and selected for further analysis. Furthermore, functional annotation showed that the mRNAs were associated with DNA repair and cell division. The protein-protein interaction (PPI) network analysis revealed that the ten highest-degree genes were considered to be hub genes. Subsequently, a competing endogenous RNA (ceRNA) network analysis and real-time PCR validation indicated that hsa_circ_0001955/hsa-miR-6719-3p/CDK1, hsa_circ_0001955/hsa-miR-1277-5p/NEDD4L and hsa_circ_0003954/hsa-miR-15a-3p/SYCP2 were highly correlated with cervical carcinogenesis. Silencing of hsa_circ_0003954 inhibited SiHa cell proliferation and perturb the cell cycle in vitro. This study provides insight into the molecular events regulating cervical carcinogenesis, identifies functional circRNAs in CSCC, and improves the understanding of the pathogenesis and molecular biomarkers of CSCC and HSIL.
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spelling pubmed-84382392021-09-15 Comprehensive Analysis of circRNA Expression Profiles During Cervical Carcinogenesis Luo, Haixia Li, Yuanxing Zhao, Yueyang Chang, Jingjing Zhang, Xiu Zou, Binbin Gao, Lifang Wang, Wei Front Oncol Oncology Circular RNAs (circRNAs) are regulatory molecules that participate in the occurrence, development and progression of tumors. To obtain a complete blueprint of cervical carcinogenesis, we analyzed the temporal transcriptomic landscapes of mRNAs and circRNAs. Microarrays were performed to identify the circRNA and mRNA expression profiles of cervical squamous cell carcinoma (CSCC) and high-grade squamous intraepithelial lesion (HSIL) patients compared with normal controls (NC). Short time-series expression miner (STEM) was utilized to characterize the time-course expression patterns of circRNAs and mRNAs from NC to HSIL and CSCC. A total of 3 circRNA profiles and 3 mRNA profiles with continuous upregulated patterns were identified and selected for further analysis. Furthermore, functional annotation showed that the mRNAs were associated with DNA repair and cell division. The protein-protein interaction (PPI) network analysis revealed that the ten highest-degree genes were considered to be hub genes. Subsequently, a competing endogenous RNA (ceRNA) network analysis and real-time PCR validation indicated that hsa_circ_0001955/hsa-miR-6719-3p/CDK1, hsa_circ_0001955/hsa-miR-1277-5p/NEDD4L and hsa_circ_0003954/hsa-miR-15a-3p/SYCP2 were highly correlated with cervical carcinogenesis. Silencing of hsa_circ_0003954 inhibited SiHa cell proliferation and perturb the cell cycle in vitro. This study provides insight into the molecular events regulating cervical carcinogenesis, identifies functional circRNAs in CSCC, and improves the understanding of the pathogenesis and molecular biomarkers of CSCC and HSIL. Frontiers Media S.A. 2021-08-31 /pmc/articles/PMC8438239/ /pubmed/34532284 http://dx.doi.org/10.3389/fonc.2021.676609 Text en Copyright © 2021 Luo, Li, Zhao, Chang, Zhang, Zou, Gao and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Luo, Haixia
Li, Yuanxing
Zhao, Yueyang
Chang, Jingjing
Zhang, Xiu
Zou, Binbin
Gao, Lifang
Wang, Wei
Comprehensive Analysis of circRNA Expression Profiles During Cervical Carcinogenesis
title Comprehensive Analysis of circRNA Expression Profiles During Cervical Carcinogenesis
title_full Comprehensive Analysis of circRNA Expression Profiles During Cervical Carcinogenesis
title_fullStr Comprehensive Analysis of circRNA Expression Profiles During Cervical Carcinogenesis
title_full_unstemmed Comprehensive Analysis of circRNA Expression Profiles During Cervical Carcinogenesis
title_short Comprehensive Analysis of circRNA Expression Profiles During Cervical Carcinogenesis
title_sort comprehensive analysis of circrna expression profiles during cervical carcinogenesis
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438239/
https://www.ncbi.nlm.nih.gov/pubmed/34532284
http://dx.doi.org/10.3389/fonc.2021.676609
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