Cargando…

Sec62 Regulates Endoplasmic Reticulum Stress and Autophagy Balance to Affect Foot-and-Mouth Disease Virus Replication

Endoplasmic reticulum (ER) stress-induced autophagy is closely associated with viral infection and propagation. However, the intrinsic link between ER stress, autophagy, and viral replication during foot-and-mouth disease virus (FMDV) infection is not fully elucidated. Our previous studies demonstra...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Jin’en, Zhang, Zhihui, Teng, Zhidong, Abdullah, Sahibzada Waheed, Sun, Shiqi, Guo, Huichen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438241/
https://www.ncbi.nlm.nih.gov/pubmed/34532300
http://dx.doi.org/10.3389/fcimb.2021.707107
_version_ 1783752327913537536
author Wu, Jin’en
Zhang, Zhihui
Teng, Zhidong
Abdullah, Sahibzada Waheed
Sun, Shiqi
Guo, Huichen
author_facet Wu, Jin’en
Zhang, Zhihui
Teng, Zhidong
Abdullah, Sahibzada Waheed
Sun, Shiqi
Guo, Huichen
author_sort Wu, Jin’en
collection PubMed
description Endoplasmic reticulum (ER) stress-induced autophagy is closely associated with viral infection and propagation. However, the intrinsic link between ER stress, autophagy, and viral replication during foot-and-mouth disease virus (FMDV) infection is not fully elucidated. Our previous studies demonstrated that FMDV infection activated the ER stress-associated UPR of the PERK-eIF2a and ATF6 signaling pathway, whereas the IRE1a signaling was suppressed. We found that the activated-ATF6 pathway participated in FMDV-induced autophagy and FMDV replication, while the IRE1α pathway only affected FMDV replication. Further studies indicated that Sec62 was greatly reduced in the later stages of FMDV infection and blocked the activation of the autophagy-related IRE1α-JNK pathway. Moreover, it was also found that Sec62 promoted IRE1a phosphorylation and negatively regulated FMDV proliferation. Importantly, Sec62 may interact with LC3 to regulate ER stress and autophagy balance and eventually contribute to FMDV clearance via fusing with lysosomes. Altogether, these results suggest that Sec62 is a critical molecule in maintaining and recovering ER homeostasis by activating the IRE1α-JNK pathway and delivering autophagosome into the lysosome, thus providing new insights on FMDV-host interactions and novel antiviral therapies.
format Online
Article
Text
id pubmed-8438241
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-84382412021-09-15 Sec62 Regulates Endoplasmic Reticulum Stress and Autophagy Balance to Affect Foot-and-Mouth Disease Virus Replication Wu, Jin’en Zhang, Zhihui Teng, Zhidong Abdullah, Sahibzada Waheed Sun, Shiqi Guo, Huichen Front Cell Infect Microbiol Cellular and Infection Microbiology Endoplasmic reticulum (ER) stress-induced autophagy is closely associated with viral infection and propagation. However, the intrinsic link between ER stress, autophagy, and viral replication during foot-and-mouth disease virus (FMDV) infection is not fully elucidated. Our previous studies demonstrated that FMDV infection activated the ER stress-associated UPR of the PERK-eIF2a and ATF6 signaling pathway, whereas the IRE1a signaling was suppressed. We found that the activated-ATF6 pathway participated in FMDV-induced autophagy and FMDV replication, while the IRE1α pathway only affected FMDV replication. Further studies indicated that Sec62 was greatly reduced in the later stages of FMDV infection and blocked the activation of the autophagy-related IRE1α-JNK pathway. Moreover, it was also found that Sec62 promoted IRE1a phosphorylation and negatively regulated FMDV proliferation. Importantly, Sec62 may interact with LC3 to regulate ER stress and autophagy balance and eventually contribute to FMDV clearance via fusing with lysosomes. Altogether, these results suggest that Sec62 is a critical molecule in maintaining and recovering ER homeostasis by activating the IRE1α-JNK pathway and delivering autophagosome into the lysosome, thus providing new insights on FMDV-host interactions and novel antiviral therapies. Frontiers Media S.A. 2021-08-31 /pmc/articles/PMC8438241/ /pubmed/34532300 http://dx.doi.org/10.3389/fcimb.2021.707107 Text en Copyright © 2021 Wu, Zhang, Teng, Abdullah, Sun and Guo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Wu, Jin’en
Zhang, Zhihui
Teng, Zhidong
Abdullah, Sahibzada Waheed
Sun, Shiqi
Guo, Huichen
Sec62 Regulates Endoplasmic Reticulum Stress and Autophagy Balance to Affect Foot-and-Mouth Disease Virus Replication
title Sec62 Regulates Endoplasmic Reticulum Stress and Autophagy Balance to Affect Foot-and-Mouth Disease Virus Replication
title_full Sec62 Regulates Endoplasmic Reticulum Stress and Autophagy Balance to Affect Foot-and-Mouth Disease Virus Replication
title_fullStr Sec62 Regulates Endoplasmic Reticulum Stress and Autophagy Balance to Affect Foot-and-Mouth Disease Virus Replication
title_full_unstemmed Sec62 Regulates Endoplasmic Reticulum Stress and Autophagy Balance to Affect Foot-and-Mouth Disease Virus Replication
title_short Sec62 Regulates Endoplasmic Reticulum Stress and Autophagy Balance to Affect Foot-and-Mouth Disease Virus Replication
title_sort sec62 regulates endoplasmic reticulum stress and autophagy balance to affect foot-and-mouth disease virus replication
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438241/
https://www.ncbi.nlm.nih.gov/pubmed/34532300
http://dx.doi.org/10.3389/fcimb.2021.707107
work_keys_str_mv AT wujinen sec62regulatesendoplasmicreticulumstressandautophagybalancetoaffectfootandmouthdiseasevirusreplication
AT zhangzhihui sec62regulatesendoplasmicreticulumstressandautophagybalancetoaffectfootandmouthdiseasevirusreplication
AT tengzhidong sec62regulatesendoplasmicreticulumstressandautophagybalancetoaffectfootandmouthdiseasevirusreplication
AT abdullahsahibzadawaheed sec62regulatesendoplasmicreticulumstressandautophagybalancetoaffectfootandmouthdiseasevirusreplication
AT sunshiqi sec62regulatesendoplasmicreticulumstressandautophagybalancetoaffectfootandmouthdiseasevirusreplication
AT guohuichen sec62regulatesendoplasmicreticulumstressandautophagybalancetoaffectfootandmouthdiseasevirusreplication