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Transcriptome and Metabolome Analyses of the Flowers and Leaves of Chrysanthemum dichrum

Chrysanthemum dichrum is an important wild species in the family Asteraceae. However, because of a lack of genetic information, there has been relatively little research conducted on the molecular mechanisms in C. dichrum. There is no report describing the transcriptome and metabolome of C. dichrum...

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Autores principales: Liu, Hua, Chen, Xiaoxi, Chen, Hạixia, Lu, Jie, Chen, Dongliang, Luo, Chang, Cheng, Xi, Jia, Yin, Huang, Conglin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438430/
https://www.ncbi.nlm.nih.gov/pubmed/34531898
http://dx.doi.org/10.3389/fgene.2021.716163
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author Liu, Hua
Chen, Xiaoxi
Chen, Hạixia
Lu, Jie
Chen, Dongliang
Luo, Chang
Cheng, Xi
Jia, Yin
Huang, Conglin
author_facet Liu, Hua
Chen, Xiaoxi
Chen, Hạixia
Lu, Jie
Chen, Dongliang
Luo, Chang
Cheng, Xi
Jia, Yin
Huang, Conglin
author_sort Liu, Hua
collection PubMed
description Chrysanthemum dichrum is an important wild species in the family Asteraceae. However, because of a lack of genetic information, there has been relatively little research conducted on the molecular mechanisms in C. dichrum. There is no report describing the transcriptome and metabolome of C. dichrum flowers and leaves at different developmental stages. In this study, high-throughput sequencing and RNA-seq analyses were used to investigate the transcriptome of C. dichrum leaves, flower buds, and blooming flowers. Additionally, these three tissues also underwent a metabolomics analysis. A total of 447,313,764 clean reads were assembled into 77,683 unigenes, with an average length of 839 bp. Of the 44,204 annotated unigenes, 42,189, 28,531, 23,420, and 17,599 were annotated using the Nr, Swiss-Prot, KOG, and KEGG databases, respectively. Furthermore, 31,848 differentially expressed genes (DEGs) were detected between the leaves and flower buds, whereas 23,197 DEGs were detected between the leaves and blooming flowers, and 11,240 DEGs were detected between the flower buds and blooming flowers. Finally, a quantitative real-time Polymerase Chain Reaction (qRT-PCR) assay was conducted to validate the identified DEGs. The metabolome data revealed several abundant metabolites in C. dichrum leaves, flower buds, and blooming flowers, including raffinose, 1-kestose, asparagine, glutamine, and other medicinal compounds. The expression patterns of significant DEGs revealed by the transcriptome analysis as well as the data for the differentially abundant metabolites in three C. dichrum tissues provide important genetic and metabolic information relevant for future investigations of the molecular mechanisms in C. dichrum. Moreover, the results of this study may be useful for the molecular breeding, development, and application of C. dichrum resources.
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spelling pubmed-84384302021-09-15 Transcriptome and Metabolome Analyses of the Flowers and Leaves of Chrysanthemum dichrum Liu, Hua Chen, Xiaoxi Chen, Hạixia Lu, Jie Chen, Dongliang Luo, Chang Cheng, Xi Jia, Yin Huang, Conglin Front Genet Genetics Chrysanthemum dichrum is an important wild species in the family Asteraceae. However, because of a lack of genetic information, there has been relatively little research conducted on the molecular mechanisms in C. dichrum. There is no report describing the transcriptome and metabolome of C. dichrum flowers and leaves at different developmental stages. In this study, high-throughput sequencing and RNA-seq analyses were used to investigate the transcriptome of C. dichrum leaves, flower buds, and blooming flowers. Additionally, these three tissues also underwent a metabolomics analysis. A total of 447,313,764 clean reads were assembled into 77,683 unigenes, with an average length of 839 bp. Of the 44,204 annotated unigenes, 42,189, 28,531, 23,420, and 17,599 were annotated using the Nr, Swiss-Prot, KOG, and KEGG databases, respectively. Furthermore, 31,848 differentially expressed genes (DEGs) were detected between the leaves and flower buds, whereas 23,197 DEGs were detected between the leaves and blooming flowers, and 11,240 DEGs were detected between the flower buds and blooming flowers. Finally, a quantitative real-time Polymerase Chain Reaction (qRT-PCR) assay was conducted to validate the identified DEGs. The metabolome data revealed several abundant metabolites in C. dichrum leaves, flower buds, and blooming flowers, including raffinose, 1-kestose, asparagine, glutamine, and other medicinal compounds. The expression patterns of significant DEGs revealed by the transcriptome analysis as well as the data for the differentially abundant metabolites in three C. dichrum tissues provide important genetic and metabolic information relevant for future investigations of the molecular mechanisms in C. dichrum. Moreover, the results of this study may be useful for the molecular breeding, development, and application of C. dichrum resources. Frontiers Media S.A. 2021-08-31 /pmc/articles/PMC8438430/ /pubmed/34531898 http://dx.doi.org/10.3389/fgene.2021.716163 Text en Copyright © 2021 Liu, Chen, Chen, Lu, Chen, Luo, Cheng, Jia and Huang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Liu, Hua
Chen, Xiaoxi
Chen, Hạixia
Lu, Jie
Chen, Dongliang
Luo, Chang
Cheng, Xi
Jia, Yin
Huang, Conglin
Transcriptome and Metabolome Analyses of the Flowers and Leaves of Chrysanthemum dichrum
title Transcriptome and Metabolome Analyses of the Flowers and Leaves of Chrysanthemum dichrum
title_full Transcriptome and Metabolome Analyses of the Flowers and Leaves of Chrysanthemum dichrum
title_fullStr Transcriptome and Metabolome Analyses of the Flowers and Leaves of Chrysanthemum dichrum
title_full_unstemmed Transcriptome and Metabolome Analyses of the Flowers and Leaves of Chrysanthemum dichrum
title_short Transcriptome and Metabolome Analyses of the Flowers and Leaves of Chrysanthemum dichrum
title_sort transcriptome and metabolome analyses of the flowers and leaves of chrysanthemum dichrum
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438430/
https://www.ncbi.nlm.nih.gov/pubmed/34531898
http://dx.doi.org/10.3389/fgene.2021.716163
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