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Mast cell degranulation and vascular endothelial growth factor expression in mouse skin following ionizing irradiation
The present study aimed to identify the mechanisms underlying the increase in vascular permeability in mouse skin following irradiation. The left ears of C3H mice were subjected to 2 and 15 Gy of radiation in a single exposure. At 24 h after irradiation, the ears were excised and tissue sections wer...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438482/ https://www.ncbi.nlm.nih.gov/pubmed/34350962 http://dx.doi.org/10.1093/jrr/rrab067 |
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author | Lee, Chang Geol Moon, Sun Rock Cho, Mee Yon Park, Kyung Ran |
author_facet | Lee, Chang Geol Moon, Sun Rock Cho, Mee Yon Park, Kyung Ran |
author_sort | Lee, Chang Geol |
collection | PubMed |
description | The present study aimed to identify the mechanisms underlying the increase in vascular permeability in mouse skin following irradiation. The left ears of C3H mice were subjected to 2 and 15 Gy of radiation in a single exposure. At 24 h after irradiation, the ears were excised and tissue sections were stained with toluidine blue to assess mast cell degranulation. Vascular endothelial growth factor (VEGF) expression was assessed via immunohistochemistry and western blotting. Approximately 5% (3%–14%) (mean [95% CI]) of mast cells in the skin of control mice were degranulated; moreover, at 24 h after 2 Gy irradiation, this value increased to approximately 20% (17%–28%). Mast cell degranulation by 15 Gy irradiation (32% [24%–40%]) was greater than that by 2 Gy irradiation. Significant differences were observed in mast cell degranulation among the control, 2 Gy and 15 Gy groups (p = 0.012). Furthermore, VEGF-positive reactions were observed in the cytoplasm of scattered fibroblasts in the dermis. In immunohistochemistry tests, VEGF expression at 24 h after irradiation increased slightly in the 2 Gy group compared to that in the control group, whereas no difference in VEGF expression was observed in the 15 Gy group compared to that in the control group. Expression of VEGF in western blots was consistent with that in immunohistochemistry. In conclusion, mast cell degranulation was increased in mouse skin at 24 h after irradiation in a dose-dependent manner. In contrast, VEGF expression was slightly increased following only low-dose (2 Gy) irradiation. |
format | Online Article Text |
id | pubmed-8438482 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-84384822021-09-15 Mast cell degranulation and vascular endothelial growth factor expression in mouse skin following ionizing irradiation Lee, Chang Geol Moon, Sun Rock Cho, Mee Yon Park, Kyung Ran J Radiat Res Fundamental Radiation Science The present study aimed to identify the mechanisms underlying the increase in vascular permeability in mouse skin following irradiation. The left ears of C3H mice were subjected to 2 and 15 Gy of radiation in a single exposure. At 24 h after irradiation, the ears were excised and tissue sections were stained with toluidine blue to assess mast cell degranulation. Vascular endothelial growth factor (VEGF) expression was assessed via immunohistochemistry and western blotting. Approximately 5% (3%–14%) (mean [95% CI]) of mast cells in the skin of control mice were degranulated; moreover, at 24 h after 2 Gy irradiation, this value increased to approximately 20% (17%–28%). Mast cell degranulation by 15 Gy irradiation (32% [24%–40%]) was greater than that by 2 Gy irradiation. Significant differences were observed in mast cell degranulation among the control, 2 Gy and 15 Gy groups (p = 0.012). Furthermore, VEGF-positive reactions were observed in the cytoplasm of scattered fibroblasts in the dermis. In immunohistochemistry tests, VEGF expression at 24 h after irradiation increased slightly in the 2 Gy group compared to that in the control group, whereas no difference in VEGF expression was observed in the 15 Gy group compared to that in the control group. Expression of VEGF in western blots was consistent with that in immunohistochemistry. In conclusion, mast cell degranulation was increased in mouse skin at 24 h after irradiation in a dose-dependent manner. In contrast, VEGF expression was slightly increased following only low-dose (2 Gy) irradiation. Oxford University Press 2021-08-05 /pmc/articles/PMC8438482/ /pubmed/34350962 http://dx.doi.org/10.1093/jrr/rrab067 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Fundamental Radiation Science Lee, Chang Geol Moon, Sun Rock Cho, Mee Yon Park, Kyung Ran Mast cell degranulation and vascular endothelial growth factor expression in mouse skin following ionizing irradiation |
title | Mast cell degranulation and vascular endothelial growth factor expression in mouse skin following ionizing irradiation |
title_full | Mast cell degranulation and vascular endothelial growth factor expression in mouse skin following ionizing irradiation |
title_fullStr | Mast cell degranulation and vascular endothelial growth factor expression in mouse skin following ionizing irradiation |
title_full_unstemmed | Mast cell degranulation and vascular endothelial growth factor expression in mouse skin following ionizing irradiation |
title_short | Mast cell degranulation and vascular endothelial growth factor expression in mouse skin following ionizing irradiation |
title_sort | mast cell degranulation and vascular endothelial growth factor expression in mouse skin following ionizing irradiation |
topic | Fundamental Radiation Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438482/ https://www.ncbi.nlm.nih.gov/pubmed/34350962 http://dx.doi.org/10.1093/jrr/rrab067 |
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