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Nasopharyngeal colonization of otopathogens in South Indian children with acute otitis media – A case control pilot study

BACKGROUND: Acute otitis media (AOM) is an inflammatory disease of the middle ear causing significant morbidity in early childhood. A pilot study was undertaken to identify the role of various risk factors South Indian children with AOM, especially the role of nasopharyngeal otopathogens. METHODOLOG...

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Autores principales: Napolean, M., Rosemol, V., John, M., Varghese, A.M., Periyasamy, J., Balaji, V., Naina, P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Chinese PLA General Hospital 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438633/
https://www.ncbi.nlm.nih.gov/pubmed/34548867
http://dx.doi.org/10.1016/j.joto.2021.02.004
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author Napolean, M.
Rosemol, V.
John, M.
Varghese, A.M.
Periyasamy, J.
Balaji, V.
Naina, P.
author_facet Napolean, M.
Rosemol, V.
John, M.
Varghese, A.M.
Periyasamy, J.
Balaji, V.
Naina, P.
author_sort Napolean, M.
collection PubMed
description BACKGROUND: Acute otitis media (AOM) is an inflammatory disease of the middle ear causing significant morbidity in early childhood. A pilot study was undertaken to identify the role of various risk factors South Indian children with AOM, especially the role of nasopharyngeal otopathogens. METHODOLOGY: A prospective case control pilot study was conducted in children aged below six years, presenting to a single tertiary care from 2018 to 2019. Fifty cases with AOM and 45 age and gender matched controls were recruited. Two nasopharyngeal swabs were collected, one was processed for bacterial culture. The other swab was processed according to the CDC recommended broth enrichment method to identify carriage of S. pneumoniae. Subsequent serotyping was done by Quellung method and conventional sequential multiplex PCR. RESULT: Otalgia was the major presentation seen in 92% of the children with AOM. None of the clinical and demographic characteristics were found to be statistically significant between the cases and controls. The most common otopathogen was S. pneumoniae (55%) followed by H. influenza (29%). The common S. pneumoniae serotypes encountered were 11A and 19F.Nasopharyngeal colonization with S. pneumoniae [OR 6.57, p < 0.003] and H. influenzae [OR14.18, p < 0.003] were significant risk factors for AOM in children. The risk increased with co-colonization (OR 13.89,p < 0.003). CONCLUSION: This study strengthens the significant association between nasopharyngeal colonization of otopathogens and AOM as a risk factor that is enhanced by co-colonization.S. pneumoniae was the main otopathogen in this population, serotypes 11A and 19F being the most common.
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spelling pubmed-84386332021-09-20 Nasopharyngeal colonization of otopathogens in South Indian children with acute otitis media – A case control pilot study Napolean, M. Rosemol, V. John, M. Varghese, A.M. Periyasamy, J. Balaji, V. Naina, P. J Otol Research Article BACKGROUND: Acute otitis media (AOM) is an inflammatory disease of the middle ear causing significant morbidity in early childhood. A pilot study was undertaken to identify the role of various risk factors South Indian children with AOM, especially the role of nasopharyngeal otopathogens. METHODOLOGY: A prospective case control pilot study was conducted in children aged below six years, presenting to a single tertiary care from 2018 to 2019. Fifty cases with AOM and 45 age and gender matched controls were recruited. Two nasopharyngeal swabs were collected, one was processed for bacterial culture. The other swab was processed according to the CDC recommended broth enrichment method to identify carriage of S. pneumoniae. Subsequent serotyping was done by Quellung method and conventional sequential multiplex PCR. RESULT: Otalgia was the major presentation seen in 92% of the children with AOM. None of the clinical and demographic characteristics were found to be statistically significant between the cases and controls. The most common otopathogen was S. pneumoniae (55%) followed by H. influenza (29%). The common S. pneumoniae serotypes encountered were 11A and 19F.Nasopharyngeal colonization with S. pneumoniae [OR 6.57, p < 0.003] and H. influenzae [OR14.18, p < 0.003] were significant risk factors for AOM in children. The risk increased with co-colonization (OR 13.89,p < 0.003). CONCLUSION: This study strengthens the significant association between nasopharyngeal colonization of otopathogens and AOM as a risk factor that is enhanced by co-colonization.S. pneumoniae was the main otopathogen in this population, serotypes 11A and 19F being the most common. Chinese PLA General Hospital 2021-10 2021-03-04 /pmc/articles/PMC8438633/ /pubmed/34548867 http://dx.doi.org/10.1016/j.joto.2021.02.004 Text en © 2021 PLA General Hospital Department of Otolaryngology Head and Neck Surgery. Production and hosting by Elsevier (Singapore) Pte Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Napolean, M.
Rosemol, V.
John, M.
Varghese, A.M.
Periyasamy, J.
Balaji, V.
Naina, P.
Nasopharyngeal colonization of otopathogens in South Indian children with acute otitis media – A case control pilot study
title Nasopharyngeal colonization of otopathogens in South Indian children with acute otitis media – A case control pilot study
title_full Nasopharyngeal colonization of otopathogens in South Indian children with acute otitis media – A case control pilot study
title_fullStr Nasopharyngeal colonization of otopathogens in South Indian children with acute otitis media – A case control pilot study
title_full_unstemmed Nasopharyngeal colonization of otopathogens in South Indian children with acute otitis media – A case control pilot study
title_short Nasopharyngeal colonization of otopathogens in South Indian children with acute otitis media – A case control pilot study
title_sort nasopharyngeal colonization of otopathogens in south indian children with acute otitis media – a case control pilot study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438633/
https://www.ncbi.nlm.nih.gov/pubmed/34548867
http://dx.doi.org/10.1016/j.joto.2021.02.004
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