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Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay

The incidence of acute myocardial infarction (AMI) is currently increasing. Early detection is important for the treatment and prognosis of patients with AMI. Heart-type fatty acid-binding protein (H-FABP) may be used as an early marker of AMI due to its high sensitivity, specificity and prognostic...

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Autores principales: Ren, Yang-Guang, Liu, Mei-Chen, Ji, Meng-Zhen, Chen, Chen, Hu, Hang-Zhan, Wang, Zi-Xuan, Yu, Pin-Qian, Shang, Jia-Ming, Zhou, Qian-Wen, Tao, Ning-Ya, Guo, Ya-Fei, Lu, Yan-Jiao, Wang, Zhi-Zeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438696/
https://www.ncbi.nlm.nih.gov/pubmed/34539834
http://dx.doi.org/10.3892/etm.2021.10673
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author Ren, Yang-Guang
Liu, Mei-Chen
Ji, Meng-Zhen
Chen, Chen
Hu, Hang-Zhan
Wang, Zi-Xuan
Yu, Pin-Qian
Shang, Jia-Ming
Zhou, Qian-Wen
Tao, Ning-Ya
Guo, Ya-Fei
Lu, Yan-Jiao
Wang, Zhi-Zeng
author_facet Ren, Yang-Guang
Liu, Mei-Chen
Ji, Meng-Zhen
Chen, Chen
Hu, Hang-Zhan
Wang, Zi-Xuan
Yu, Pin-Qian
Shang, Jia-Ming
Zhou, Qian-Wen
Tao, Ning-Ya
Guo, Ya-Fei
Lu, Yan-Jiao
Wang, Zhi-Zeng
author_sort Ren, Yang-Guang
collection PubMed
description The incidence of acute myocardial infarction (AMI) is currently increasing. Early detection is important for the treatment and prognosis of patients with AMI. Heart-type fatty acid-binding protein (H-FABP) may be used as an early marker of AMI due to its high sensitivity, specificity and prognostic value. Therefore, in the present study, H-FABP was used as a biomarker in a double-antibody sandwich method and colloidal gold-based lateral flow immunoassay to develop a rapid detection kit for H-FABP with a processing time of only 5 min. The sensitivity of the kit in plasma and whole blood was 1 ng/ml and this method had good specificity, exhibiting no cross-reaction with cardiac troponin I, myoglobin or creatine kinase-Mb. The kits had good shelf life and stability, as they were able to be stored at 40˚C for 30 days. A total of 12 clinical samples were collected for detection and the coincidence rate with the ELISA method was up to 91.67%. Therefore, the present study provided a simple, rapid and economical early-detection in-home testing kit.
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spelling pubmed-84386962021-09-17 Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay Ren, Yang-Guang Liu, Mei-Chen Ji, Meng-Zhen Chen, Chen Hu, Hang-Zhan Wang, Zi-Xuan Yu, Pin-Qian Shang, Jia-Ming Zhou, Qian-Wen Tao, Ning-Ya Guo, Ya-Fei Lu, Yan-Jiao Wang, Zhi-Zeng Exp Ther Med Articles The incidence of acute myocardial infarction (AMI) is currently increasing. Early detection is important for the treatment and prognosis of patients with AMI. Heart-type fatty acid-binding protein (H-FABP) may be used as an early marker of AMI due to its high sensitivity, specificity and prognostic value. Therefore, in the present study, H-FABP was used as a biomarker in a double-antibody sandwich method and colloidal gold-based lateral flow immunoassay to develop a rapid detection kit for H-FABP with a processing time of only 5 min. The sensitivity of the kit in plasma and whole blood was 1 ng/ml and this method had good specificity, exhibiting no cross-reaction with cardiac troponin I, myoglobin or creatine kinase-Mb. The kits had good shelf life and stability, as they were able to be stored at 40˚C for 30 days. A total of 12 clinical samples were collected for detection and the coincidence rate with the ELISA method was up to 91.67%. Therefore, the present study provided a simple, rapid and economical early-detection in-home testing kit. D.A. Spandidos 2021-11 2021-08-31 /pmc/articles/PMC8438696/ /pubmed/34539834 http://dx.doi.org/10.3892/etm.2021.10673 Text en Copyright: © Ren et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ren, Yang-Guang
Liu, Mei-Chen
Ji, Meng-Zhen
Chen, Chen
Hu, Hang-Zhan
Wang, Zi-Xuan
Yu, Pin-Qian
Shang, Jia-Ming
Zhou, Qian-Wen
Tao, Ning-Ya
Guo, Ya-Fei
Lu, Yan-Jiao
Wang, Zhi-Zeng
Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay
title Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay
title_full Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay
title_fullStr Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay
title_full_unstemmed Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay
title_short Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay
title_sort rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438696/
https://www.ncbi.nlm.nih.gov/pubmed/34539834
http://dx.doi.org/10.3892/etm.2021.10673
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