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Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay
The incidence of acute myocardial infarction (AMI) is currently increasing. Early detection is important for the treatment and prognosis of patients with AMI. Heart-type fatty acid-binding protein (H-FABP) may be used as an early marker of AMI due to its high sensitivity, specificity and prognostic...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438696/ https://www.ncbi.nlm.nih.gov/pubmed/34539834 http://dx.doi.org/10.3892/etm.2021.10673 |
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author | Ren, Yang-Guang Liu, Mei-Chen Ji, Meng-Zhen Chen, Chen Hu, Hang-Zhan Wang, Zi-Xuan Yu, Pin-Qian Shang, Jia-Ming Zhou, Qian-Wen Tao, Ning-Ya Guo, Ya-Fei Lu, Yan-Jiao Wang, Zhi-Zeng |
author_facet | Ren, Yang-Guang Liu, Mei-Chen Ji, Meng-Zhen Chen, Chen Hu, Hang-Zhan Wang, Zi-Xuan Yu, Pin-Qian Shang, Jia-Ming Zhou, Qian-Wen Tao, Ning-Ya Guo, Ya-Fei Lu, Yan-Jiao Wang, Zhi-Zeng |
author_sort | Ren, Yang-Guang |
collection | PubMed |
description | The incidence of acute myocardial infarction (AMI) is currently increasing. Early detection is important for the treatment and prognosis of patients with AMI. Heart-type fatty acid-binding protein (H-FABP) may be used as an early marker of AMI due to its high sensitivity, specificity and prognostic value. Therefore, in the present study, H-FABP was used as a biomarker in a double-antibody sandwich method and colloidal gold-based lateral flow immunoassay to develop a rapid detection kit for H-FABP with a processing time of only 5 min. The sensitivity of the kit in plasma and whole blood was 1 ng/ml and this method had good specificity, exhibiting no cross-reaction with cardiac troponin I, myoglobin or creatine kinase-Mb. The kits had good shelf life and stability, as they were able to be stored at 40˚C for 30 days. A total of 12 clinical samples were collected for detection and the coincidence rate with the ELISA method was up to 91.67%. Therefore, the present study provided a simple, rapid and economical early-detection in-home testing kit. |
format | Online Article Text |
id | pubmed-8438696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-84386962021-09-17 Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay Ren, Yang-Guang Liu, Mei-Chen Ji, Meng-Zhen Chen, Chen Hu, Hang-Zhan Wang, Zi-Xuan Yu, Pin-Qian Shang, Jia-Ming Zhou, Qian-Wen Tao, Ning-Ya Guo, Ya-Fei Lu, Yan-Jiao Wang, Zhi-Zeng Exp Ther Med Articles The incidence of acute myocardial infarction (AMI) is currently increasing. Early detection is important for the treatment and prognosis of patients with AMI. Heart-type fatty acid-binding protein (H-FABP) may be used as an early marker of AMI due to its high sensitivity, specificity and prognostic value. Therefore, in the present study, H-FABP was used as a biomarker in a double-antibody sandwich method and colloidal gold-based lateral flow immunoassay to develop a rapid detection kit for H-FABP with a processing time of only 5 min. The sensitivity of the kit in plasma and whole blood was 1 ng/ml and this method had good specificity, exhibiting no cross-reaction with cardiac troponin I, myoglobin or creatine kinase-Mb. The kits had good shelf life and stability, as they were able to be stored at 40˚C for 30 days. A total of 12 clinical samples were collected for detection and the coincidence rate with the ELISA method was up to 91.67%. Therefore, the present study provided a simple, rapid and economical early-detection in-home testing kit. D.A. Spandidos 2021-11 2021-08-31 /pmc/articles/PMC8438696/ /pubmed/34539834 http://dx.doi.org/10.3892/etm.2021.10673 Text en Copyright: © Ren et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Ren, Yang-Guang Liu, Mei-Chen Ji, Meng-Zhen Chen, Chen Hu, Hang-Zhan Wang, Zi-Xuan Yu, Pin-Qian Shang, Jia-Ming Zhou, Qian-Wen Tao, Ning-Ya Guo, Ya-Fei Lu, Yan-Jiao Wang, Zhi-Zeng Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay |
title | Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay |
title_full | Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay |
title_fullStr | Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay |
title_full_unstemmed | Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay |
title_short | Rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay |
title_sort | rapid detection of human heart-type fatty acid-binding protein in human plasma and blood using a colloidal gold-based lateral flow immunoassay |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438696/ https://www.ncbi.nlm.nih.gov/pubmed/34539834 http://dx.doi.org/10.3892/etm.2021.10673 |
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