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Effects of ibuprofen on gene expression in chondrocytes from patients with osteoarthritis as determined by RNA-Seq
Non-steroidal anti-inflammatory drugs are a widely used symptomatic treatment in osteoarthritis (OA), but their effects on cartilage remain controversial. We studied the effects of ibuprofen on gene expression in chondrocytes from patients with OA using RNA-Seq. Chondrocytes were isolated from carti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BMJ Publishing Group
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438934/ https://www.ncbi.nlm.nih.gov/pubmed/34497153 http://dx.doi.org/10.1136/rmdopen-2021-001657 |
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author | Pemmari, Antti Tuure, Lauri Hämäläinen, Mari Leppänen, Tiina Moilanen, Teemu Moilanen, Eeva |
author_facet | Pemmari, Antti Tuure, Lauri Hämäläinen, Mari Leppänen, Tiina Moilanen, Teemu Moilanen, Eeva |
author_sort | Pemmari, Antti |
collection | PubMed |
description | Non-steroidal anti-inflammatory drugs are a widely used symptomatic treatment in osteoarthritis (OA), but their effects on cartilage remain controversial. We studied the effects of ibuprofen on gene expression in chondrocytes from patients with OA using RNA-Seq. Chondrocytes were isolated from cartilage samples of patients with OA undergoing knee replacement surgery, cultured with ibuprofen, and total mRNA was sequenced. Differentially expressed genes were identified with edgeR using pairwise comparisons. Functional analysis was performed using ingenuity pathway analysis (IPA). Ibuprofen did not induce statistically significant changes in chondrocyte transcriptome when the cells were cultured in the absence of added cytokines. In inflammatory conditions (when the cells were exposed to the OA-related cytokine interleukin (IL)-1β), 51 genes were upregulated and 42 downregulated by ibuprofen with fold change >1.5 in either direction. The upregulated genes included anti-inflammatory factors and genes associated with cell adhesion, while several mediators of inflammation were among the downregulated genes. IPA analysis revealed ibuprofen having modulating effects on inflammation-related pathways such as integrin, IL-8, ERK/MAPK and cAMP-mediated signalling pathways. In conclusion, the effects of ibuprofen on primary OA chondrocyte transcriptome appear to be neutral in normal conditions, but ibuprofen may shift chondrocyte transcriptome towards anti-inflammatory phenotype in inflammatory environments. |
format | Online Article Text |
id | pubmed-8438934 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BMJ Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-84389342021-09-24 Effects of ibuprofen on gene expression in chondrocytes from patients with osteoarthritis as determined by RNA-Seq Pemmari, Antti Tuure, Lauri Hämäläinen, Mari Leppänen, Tiina Moilanen, Teemu Moilanen, Eeva RMD Open Osteoarthritis Non-steroidal anti-inflammatory drugs are a widely used symptomatic treatment in osteoarthritis (OA), but their effects on cartilage remain controversial. We studied the effects of ibuprofen on gene expression in chondrocytes from patients with OA using RNA-Seq. Chondrocytes were isolated from cartilage samples of patients with OA undergoing knee replacement surgery, cultured with ibuprofen, and total mRNA was sequenced. Differentially expressed genes were identified with edgeR using pairwise comparisons. Functional analysis was performed using ingenuity pathway analysis (IPA). Ibuprofen did not induce statistically significant changes in chondrocyte transcriptome when the cells were cultured in the absence of added cytokines. In inflammatory conditions (when the cells were exposed to the OA-related cytokine interleukin (IL)-1β), 51 genes were upregulated and 42 downregulated by ibuprofen with fold change >1.5 in either direction. The upregulated genes included anti-inflammatory factors and genes associated with cell adhesion, while several mediators of inflammation were among the downregulated genes. IPA analysis revealed ibuprofen having modulating effects on inflammation-related pathways such as integrin, IL-8, ERK/MAPK and cAMP-mediated signalling pathways. In conclusion, the effects of ibuprofen on primary OA chondrocyte transcriptome appear to be neutral in normal conditions, but ibuprofen may shift chondrocyte transcriptome towards anti-inflammatory phenotype in inflammatory environments. BMJ Publishing Group 2021-09-08 /pmc/articles/PMC8438934/ /pubmed/34497153 http://dx.doi.org/10.1136/rmdopen-2021-001657 Text en © Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) . |
spellingShingle | Osteoarthritis Pemmari, Antti Tuure, Lauri Hämäläinen, Mari Leppänen, Tiina Moilanen, Teemu Moilanen, Eeva Effects of ibuprofen on gene expression in chondrocytes from patients with osteoarthritis as determined by RNA-Seq |
title | Effects of ibuprofen on gene expression in chondrocytes from patients with osteoarthritis as determined by RNA-Seq |
title_full | Effects of ibuprofen on gene expression in chondrocytes from patients with osteoarthritis as determined by RNA-Seq |
title_fullStr | Effects of ibuprofen on gene expression in chondrocytes from patients with osteoarthritis as determined by RNA-Seq |
title_full_unstemmed | Effects of ibuprofen on gene expression in chondrocytes from patients with osteoarthritis as determined by RNA-Seq |
title_short | Effects of ibuprofen on gene expression in chondrocytes from patients with osteoarthritis as determined by RNA-Seq |
title_sort | effects of ibuprofen on gene expression in chondrocytes from patients with osteoarthritis as determined by rna-seq |
topic | Osteoarthritis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438934/ https://www.ncbi.nlm.nih.gov/pubmed/34497153 http://dx.doi.org/10.1136/rmdopen-2021-001657 |
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