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Comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure

BACKGROUND: Post-translational modifications (PTMs) are at the heart of many cellular signaling events, which changes the function of protein. Crotonylation, one of the most important and common PTMs, plays a crucial role in the regulation of various biological processes. However, no study has evalu...

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Autores principales: Huang, Jiahuang, Tang, Donge, Zheng, Fengping, Xu, Huixuan, Dai, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8439085/
https://www.ncbi.nlm.nih.gov/pubmed/34517817
http://dx.doi.org/10.1186/s12882-021-02445-4
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author Huang, Jiahuang
Tang, Donge
Zheng, Fengping
Xu, Huixuan
Dai, Yong
author_facet Huang, Jiahuang
Tang, Donge
Zheng, Fengping
Xu, Huixuan
Dai, Yong
author_sort Huang, Jiahuang
collection PubMed
description BACKGROUND: Post-translational modifications (PTMs) are at the heart of many cellular signaling events, which changes the function of protein. Crotonylation, one of the most important and common PTMs, plays a crucial role in the regulation of various biological processes. However, no study has evaluated the role of lysine crotonylation modification in chronic renal failure (CRF) patients. METHODS: Here, we comparatively evaluated the crotonylation proteome of normal controls and chronic renal failure patients using liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with highly sensitive immune-affinity purification. RESULTS: A total of 1109 lysine modification sites were identified, of which 772 sites were up-regulated and 69 sites were down-regulated. This suggested that crotonylation modification maintains high levels in the patients with chronic renal failure. Gene ontology(GO) enrichment analysis showed that the crotonylated proteins were significantly enriched in the platelet alpha granule lumen, platelet degradulation, and cell adhesion molecule binding. In addition, Kyoto Encyclopedia of Genes and Genomes (KEGG)-based functional enrichment analysis in the Kyoto encyclopedia showed that crotonylated protein was enriched in CD36, which is closely linked to renal failure. CONCLUSIONS: This is the first report of the global crotonylation proteome in chronic renal failure patients. Crotonylation of histone and non-histone may play important roles in delaying the continuous deterioration of renal function in patients with chronic renal failure. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12882-021-02445-4.
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spelling pubmed-84390852021-09-14 Comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure Huang, Jiahuang Tang, Donge Zheng, Fengping Xu, Huixuan Dai, Yong BMC Nephrol Research BACKGROUND: Post-translational modifications (PTMs) are at the heart of many cellular signaling events, which changes the function of protein. Crotonylation, one of the most important and common PTMs, plays a crucial role in the regulation of various biological processes. However, no study has evaluated the role of lysine crotonylation modification in chronic renal failure (CRF) patients. METHODS: Here, we comparatively evaluated the crotonylation proteome of normal controls and chronic renal failure patients using liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with highly sensitive immune-affinity purification. RESULTS: A total of 1109 lysine modification sites were identified, of which 772 sites were up-regulated and 69 sites were down-regulated. This suggested that crotonylation modification maintains high levels in the patients with chronic renal failure. Gene ontology(GO) enrichment analysis showed that the crotonylated proteins were significantly enriched in the platelet alpha granule lumen, platelet degradulation, and cell adhesion molecule binding. In addition, Kyoto Encyclopedia of Genes and Genomes (KEGG)-based functional enrichment analysis in the Kyoto encyclopedia showed that crotonylated protein was enriched in CD36, which is closely linked to renal failure. CONCLUSIONS: This is the first report of the global crotonylation proteome in chronic renal failure patients. Crotonylation of histone and non-histone may play important roles in delaying the continuous deterioration of renal function in patients with chronic renal failure. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12882-021-02445-4. BioMed Central 2021-09-13 /pmc/articles/PMC8439085/ /pubmed/34517817 http://dx.doi.org/10.1186/s12882-021-02445-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Huang, Jiahuang
Tang, Donge
Zheng, Fengping
Xu, Huixuan
Dai, Yong
Comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure
title Comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure
title_full Comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure
title_fullStr Comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure
title_full_unstemmed Comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure
title_short Comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure
title_sort comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8439085/
https://www.ncbi.nlm.nih.gov/pubmed/34517817
http://dx.doi.org/10.1186/s12882-021-02445-4
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