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Association between Oral Pathology, Carotid Stenosis, and Oral Bacterial DNA in Cerebral Thrombi of Patients with Stroke

METHODS: Thrombus aspirates and control arterial blood were taken from 71 patients (70.4% male; mean age, 67.4 years) with acute ischemic stroke. Tooth pathology was registered using CT scans. Carotid stenosis was estimated with CTA and ultrasonography. The presence of bacterial DNA from aspirated t...

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Autores principales: Patrakka, Olli, Mehtonen, Helena, Tuomisto, Sari, Pienimäki, Juha-Pekka, Ollikainen, Jyrki, Huhtala, Heini, Pessi, Tanja, Oksala, Niku, Lehtimäki, Terho, Järnstedt, Jorma, Martiskainen, Mika, Karhunen, Pekka J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440111/
https://www.ncbi.nlm.nih.gov/pubmed/34531969
http://dx.doi.org/10.1155/2021/5402764
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author Patrakka, Olli
Mehtonen, Helena
Tuomisto, Sari
Pienimäki, Juha-Pekka
Ollikainen, Jyrki
Huhtala, Heini
Pessi, Tanja
Oksala, Niku
Lehtimäki, Terho
Järnstedt, Jorma
Martiskainen, Mika
Karhunen, Pekka J.
author_facet Patrakka, Olli
Mehtonen, Helena
Tuomisto, Sari
Pienimäki, Juha-Pekka
Ollikainen, Jyrki
Huhtala, Heini
Pessi, Tanja
Oksala, Niku
Lehtimäki, Terho
Järnstedt, Jorma
Martiskainen, Mika
Karhunen, Pekka J.
author_sort Patrakka, Olli
collection PubMed
description METHODS: Thrombus aspirates and control arterial blood were taken from 71 patients (70.4% male; mean age, 67.4 years) with acute ischemic stroke. Tooth pathology was registered using CT scans. Carotid stenosis was estimated with CTA and ultrasonography. The presence of bacterial DNA from aspirated thrombi was determined using quantitative PCR. We also analyzed the presence of these bacterial DNAs in carotid endarterectomies from patients with peripheral arterial disease. RESULTS: Bacterial DNA was found in 59 (83.1%) of the thrombus aspirates (median, 8.6-fold). Oral streptococcal DNA was found in 56 (78.9%) of the thrombus aspirates (median, 5.1-fold). DNA from A. actinomycetemcomitans and P. gingivalis was not found. Most patients suffered from poor oral health and had in median 19.0 teeth left. Paradoxically, patients with better oral health had more oral streptococcal DNA in their thrombus than the group with the worst pathology (p = 0.028). There was a trend (OR 7.122; p = 0.083) in the association of ≥50% carotid artery stenosis with more severe dental pathology. Oral streptococcal DNA was detected in 2/6 of carotid endarterectomies. CONCLUSIONS: Stroke patients had poor oral health which tended to associate with their carotid artery stenosis. Although oral streptococcal DNA was found in thrombus aspirates and carotid endarterectomy samples, the amount of oral streptococcal DNA in thrombus aspirates was the lowest among those with the most severe oral pathology. These results suggest that the association between poor oral health and acute ischemic stroke is linked to carotid artery atherosclerosis.
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spelling pubmed-84401112021-09-15 Association between Oral Pathology, Carotid Stenosis, and Oral Bacterial DNA in Cerebral Thrombi of Patients with Stroke Patrakka, Olli Mehtonen, Helena Tuomisto, Sari Pienimäki, Juha-Pekka Ollikainen, Jyrki Huhtala, Heini Pessi, Tanja Oksala, Niku Lehtimäki, Terho Järnstedt, Jorma Martiskainen, Mika Karhunen, Pekka J. Stroke Res Treat Research Article METHODS: Thrombus aspirates and control arterial blood were taken from 71 patients (70.4% male; mean age, 67.4 years) with acute ischemic stroke. Tooth pathology was registered using CT scans. Carotid stenosis was estimated with CTA and ultrasonography. The presence of bacterial DNA from aspirated thrombi was determined using quantitative PCR. We also analyzed the presence of these bacterial DNAs in carotid endarterectomies from patients with peripheral arterial disease. RESULTS: Bacterial DNA was found in 59 (83.1%) of the thrombus aspirates (median, 8.6-fold). Oral streptococcal DNA was found in 56 (78.9%) of the thrombus aspirates (median, 5.1-fold). DNA from A. actinomycetemcomitans and P. gingivalis was not found. Most patients suffered from poor oral health and had in median 19.0 teeth left. Paradoxically, patients with better oral health had more oral streptococcal DNA in their thrombus than the group with the worst pathology (p = 0.028). There was a trend (OR 7.122; p = 0.083) in the association of ≥50% carotid artery stenosis with more severe dental pathology. Oral streptococcal DNA was detected in 2/6 of carotid endarterectomies. CONCLUSIONS: Stroke patients had poor oral health which tended to associate with their carotid artery stenosis. Although oral streptococcal DNA was found in thrombus aspirates and carotid endarterectomy samples, the amount of oral streptococcal DNA in thrombus aspirates was the lowest among those with the most severe oral pathology. These results suggest that the association between poor oral health and acute ischemic stroke is linked to carotid artery atherosclerosis. Hindawi 2021-09-07 /pmc/articles/PMC8440111/ /pubmed/34531969 http://dx.doi.org/10.1155/2021/5402764 Text en Copyright © 2021 Olli Patrakka et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Patrakka, Olli
Mehtonen, Helena
Tuomisto, Sari
Pienimäki, Juha-Pekka
Ollikainen, Jyrki
Huhtala, Heini
Pessi, Tanja
Oksala, Niku
Lehtimäki, Terho
Järnstedt, Jorma
Martiskainen, Mika
Karhunen, Pekka J.
Association between Oral Pathology, Carotid Stenosis, and Oral Bacterial DNA in Cerebral Thrombi of Patients with Stroke
title Association between Oral Pathology, Carotid Stenosis, and Oral Bacterial DNA in Cerebral Thrombi of Patients with Stroke
title_full Association between Oral Pathology, Carotid Stenosis, and Oral Bacterial DNA in Cerebral Thrombi of Patients with Stroke
title_fullStr Association between Oral Pathology, Carotid Stenosis, and Oral Bacterial DNA in Cerebral Thrombi of Patients with Stroke
title_full_unstemmed Association between Oral Pathology, Carotid Stenosis, and Oral Bacterial DNA in Cerebral Thrombi of Patients with Stroke
title_short Association between Oral Pathology, Carotid Stenosis, and Oral Bacterial DNA in Cerebral Thrombi of Patients with Stroke
title_sort association between oral pathology, carotid stenosis, and oral bacterial dna in cerebral thrombi of patients with stroke
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440111/
https://www.ncbi.nlm.nih.gov/pubmed/34531969
http://dx.doi.org/10.1155/2021/5402764
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