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Genetic architecture underlying the expression of eight α-amylase trypsin inhibitors

KEY MESSAGE: Wheat cultivars largely differ in the content and composition of ATI proteins, but heritability was quite low for six out of eight ATIs. The genetic architecture of ATI proteins is built up of few major and numerous small effect QTL. ABSTRACT: Amylase trypsin inhibitors (ATIs) are impor...

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Autores principales: EL Hassouni , Khaoula, Sielaff, Malte, Curella, Valentina, Neerukonda, Manjusha, Leiser, Willmar, Würschum, Tobias, Schuppan, Detlef, Tenzer, Stefan, Longin, C. Friedrich H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440294/
https://www.ncbi.nlm.nih.gov/pubmed/34245321
http://dx.doi.org/10.1007/s00122-021-03906-y
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author EL Hassouni , Khaoula
Sielaff, Malte
Curella, Valentina
Neerukonda, Manjusha
Leiser, Willmar
Würschum, Tobias
Schuppan, Detlef
Tenzer, Stefan
Longin, C. Friedrich H.
author_facet EL Hassouni , Khaoula
Sielaff, Malte
Curella, Valentina
Neerukonda, Manjusha
Leiser, Willmar
Würschum, Tobias
Schuppan, Detlef
Tenzer, Stefan
Longin, C. Friedrich H.
author_sort EL Hassouni , Khaoula
collection PubMed
description KEY MESSAGE: Wheat cultivars largely differ in the content and composition of ATI proteins, but heritability was quite low for six out of eight ATIs. The genetic architecture of ATI proteins is built up of few major and numerous small effect QTL. ABSTRACT: Amylase trypsin inhibitors (ATIs) are important allergens in baker’s asthma and suspected triggers of non-celiac wheat sensitivity (NCWS) inducing intestinal and extra-intestinal inflammation. As studies on the expression and genetic architecture of ATI proteins in wheat are lacking, we evaluated 149 European old and modern bread wheat cultivars grown at three different field locations for their content of eight ATI proteins. Large differences in the content and composition of ATIs in the different cultivars were identified ranging from 3.76 pmol for ATI CM2 to 80.4 pmol for ATI 0.19, with up to 2.5-fold variation in CM-type and up to sixfold variation in mono/dimeric ATIs. Generally, heritability estimates were low except for ATI 0.28 and ATI CM2. ATI protein content showed a low correlation with quality traits commonly analyzed in wheat breeding. Similarly, no trends were found regarding ATI content in wheat cultivars originating from numerous countries and decades of breeding history. Genome-wide association mapping revealed a complex genetic architecture built of many small, few medium and two major quantitative trait loci (QTL). The major QTL were located on chromosomes 3B for ATI 0.19-like and 6B for ATI 0.28, explaining 70.6 and 68.7% of the genotypic variance, respectively. Within close physical proximity to the medium and major QTL, we identified eight potential candidate genes on the wheat reference genome encoding structurally related lipid transfer proteins. Consequently, selection and breeding of wheat cultivars with low ATI protein amounts appear difficult requiring other strategies to reduce ATI content in wheat products. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00122-021-03906-y.
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spelling pubmed-84402942021-10-01 Genetic architecture underlying the expression of eight α-amylase trypsin inhibitors EL Hassouni , Khaoula Sielaff, Malte Curella, Valentina Neerukonda, Manjusha Leiser, Willmar Würschum, Tobias Schuppan, Detlef Tenzer, Stefan Longin, C. Friedrich H. Theor Appl Genet Original Article KEY MESSAGE: Wheat cultivars largely differ in the content and composition of ATI proteins, but heritability was quite low for six out of eight ATIs. The genetic architecture of ATI proteins is built up of few major and numerous small effect QTL. ABSTRACT: Amylase trypsin inhibitors (ATIs) are important allergens in baker’s asthma and suspected triggers of non-celiac wheat sensitivity (NCWS) inducing intestinal and extra-intestinal inflammation. As studies on the expression and genetic architecture of ATI proteins in wheat are lacking, we evaluated 149 European old and modern bread wheat cultivars grown at three different field locations for their content of eight ATI proteins. Large differences in the content and composition of ATIs in the different cultivars were identified ranging from 3.76 pmol for ATI CM2 to 80.4 pmol for ATI 0.19, with up to 2.5-fold variation in CM-type and up to sixfold variation in mono/dimeric ATIs. Generally, heritability estimates were low except for ATI 0.28 and ATI CM2. ATI protein content showed a low correlation with quality traits commonly analyzed in wheat breeding. Similarly, no trends were found regarding ATI content in wheat cultivars originating from numerous countries and decades of breeding history. Genome-wide association mapping revealed a complex genetic architecture built of many small, few medium and two major quantitative trait loci (QTL). The major QTL were located on chromosomes 3B for ATI 0.19-like and 6B for ATI 0.28, explaining 70.6 and 68.7% of the genotypic variance, respectively. Within close physical proximity to the medium and major QTL, we identified eight potential candidate genes on the wheat reference genome encoding structurally related lipid transfer proteins. Consequently, selection and breeding of wheat cultivars with low ATI protein amounts appear difficult requiring other strategies to reduce ATI content in wheat products. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00122-021-03906-y. Springer Berlin Heidelberg 2021-07-10 2021 /pmc/articles/PMC8440294/ /pubmed/34245321 http://dx.doi.org/10.1007/s00122-021-03906-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
EL Hassouni , Khaoula
Sielaff, Malte
Curella, Valentina
Neerukonda, Manjusha
Leiser, Willmar
Würschum, Tobias
Schuppan, Detlef
Tenzer, Stefan
Longin, C. Friedrich H.
Genetic architecture underlying the expression of eight α-amylase trypsin inhibitors
title Genetic architecture underlying the expression of eight α-amylase trypsin inhibitors
title_full Genetic architecture underlying the expression of eight α-amylase trypsin inhibitors
title_fullStr Genetic architecture underlying the expression of eight α-amylase trypsin inhibitors
title_full_unstemmed Genetic architecture underlying the expression of eight α-amylase trypsin inhibitors
title_short Genetic architecture underlying the expression of eight α-amylase trypsin inhibitors
title_sort genetic architecture underlying the expression of eight α-amylase trypsin inhibitors
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440294/
https://www.ncbi.nlm.nih.gov/pubmed/34245321
http://dx.doi.org/10.1007/s00122-021-03906-y
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