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Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii
Trichosporon asahii is a pathogenic fungus that causes severe, deep-seated fungal infections in neutropenic patients. Elucidating the infection mechanisms of T. asahii based on genetic studies requires a specific gene-targeting system. Here, we established an efficient gene-targeting system in a hig...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440527/ https://www.ncbi.nlm.nih.gov/pubmed/34521867 http://dx.doi.org/10.1038/s41598-021-97287-3 |
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author | Matsumoto, Yasuhiko Nagamachi, Tae Yoshikawa, Asami Yamazaki, Hideki Yamasaki, Yusuke Yamada, Tsuyoshi Sugita, Takashi |
author_facet | Matsumoto, Yasuhiko Nagamachi, Tae Yoshikawa, Asami Yamazaki, Hideki Yamasaki, Yusuke Yamada, Tsuyoshi Sugita, Takashi |
author_sort | Matsumoto, Yasuhiko |
collection | PubMed |
description | Trichosporon asahii is a pathogenic fungus that causes severe, deep-seated fungal infections in neutropenic patients. Elucidating the infection mechanisms of T. asahii based on genetic studies requires a specific gene-targeting system. Here, we established an efficient gene-targeting system in a highly pathogenic T. asahii strain identified using the silkworm infection model. By comparing the pathogenicity of T. asahii clinical isolates in a silkworm infection model, T. asahii MPU129 was identified as a highly pathogenic strain. Using an Agrobacterium tumefaciens-mediated gene transfer system, we obtained a T. asahii MPU129 mutant lacking the ku70 gene, which encodes the Ku70 protein involved in the non-homologous end-joining repair of DNA double-strand breaks. The ku70 gene-deficient mutant showed higher gene-targeting efficiency than the wild-type strain for constructing a mutant lacking the cnb1 gene, which encodes the beta-subunit of calcineurin. The cnb1 gene-deficient mutant showed reduced pathogenicity against silkworms compared with the parental strain. These results suggest that an efficient gene-targeting system in a highly pathogenic T. asahii strain is a useful tool for elucidating the molecular mechanisms of T. asahii infection. |
format | Online Article Text |
id | pubmed-8440527 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84405272021-09-15 Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii Matsumoto, Yasuhiko Nagamachi, Tae Yoshikawa, Asami Yamazaki, Hideki Yamasaki, Yusuke Yamada, Tsuyoshi Sugita, Takashi Sci Rep Article Trichosporon asahii is a pathogenic fungus that causes severe, deep-seated fungal infections in neutropenic patients. Elucidating the infection mechanisms of T. asahii based on genetic studies requires a specific gene-targeting system. Here, we established an efficient gene-targeting system in a highly pathogenic T. asahii strain identified using the silkworm infection model. By comparing the pathogenicity of T. asahii clinical isolates in a silkworm infection model, T. asahii MPU129 was identified as a highly pathogenic strain. Using an Agrobacterium tumefaciens-mediated gene transfer system, we obtained a T. asahii MPU129 mutant lacking the ku70 gene, which encodes the Ku70 protein involved in the non-homologous end-joining repair of DNA double-strand breaks. The ku70 gene-deficient mutant showed higher gene-targeting efficiency than the wild-type strain for constructing a mutant lacking the cnb1 gene, which encodes the beta-subunit of calcineurin. The cnb1 gene-deficient mutant showed reduced pathogenicity against silkworms compared with the parental strain. These results suggest that an efficient gene-targeting system in a highly pathogenic T. asahii strain is a useful tool for elucidating the molecular mechanisms of T. asahii infection. Nature Publishing Group UK 2021-09-14 /pmc/articles/PMC8440527/ /pubmed/34521867 http://dx.doi.org/10.1038/s41598-021-97287-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Matsumoto, Yasuhiko Nagamachi, Tae Yoshikawa, Asami Yamazaki, Hideki Yamasaki, Yusuke Yamada, Tsuyoshi Sugita, Takashi Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii |
title | Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii |
title_full | Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii |
title_fullStr | Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii |
title_full_unstemmed | Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii |
title_short | Development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen Trichosporon asahii |
title_sort | development of an efficient gene-targeting system for elucidating infection mechanisms of the fungal pathogen trichosporon asahii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440527/ https://www.ncbi.nlm.nih.gov/pubmed/34521867 http://dx.doi.org/10.1038/s41598-021-97287-3 |
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