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Metabolic effect of drought stress on the leaves of young oil palm (Elaeis guineensis) plants using UHPLC–MS and multivariate analysis

The expansion of the oil palm in marginal areas can face challenges, such as water deficit, leading to an impact on palm oil production. A better understanding of the biological consequences of abiotic stresses on this crop can result from joint metabolic profiling and multivariate analysis. Metabol...

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Autores principales: Neto, Jorge Candido Rodrigues, Vieira, Letícia Rios, de Aquino Ribeiro, José Antônio, de Sousa, Carlos Antônio Ferreira, Júnior, Manoel Teixeira Souza, Abdelnur, Patrícia Verardi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440612/
https://www.ncbi.nlm.nih.gov/pubmed/34521943
http://dx.doi.org/10.1038/s41598-021-97835-x
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author Neto, Jorge Candido Rodrigues
Vieira, Letícia Rios
de Aquino Ribeiro, José Antônio
de Sousa, Carlos Antônio Ferreira
Júnior, Manoel Teixeira Souza
Abdelnur, Patrícia Verardi
author_facet Neto, Jorge Candido Rodrigues
Vieira, Letícia Rios
de Aquino Ribeiro, José Antônio
de Sousa, Carlos Antônio Ferreira
Júnior, Manoel Teixeira Souza
Abdelnur, Patrícia Verardi
author_sort Neto, Jorge Candido Rodrigues
collection PubMed
description The expansion of the oil palm in marginal areas can face challenges, such as water deficit, leading to an impact on palm oil production. A better understanding of the biological consequences of abiotic stresses on this crop can result from joint metabolic profiling and multivariate analysis. Metabolic profiling of leaves was performed from control and stressed plants (7 and 14 days of stress). Samples were extracted and analyzed on a UHPLC-ESI-Q-TOF-HRMS system. Acquired data were processed using XCMS Online and MetaboAnalyst for multivariate and pathway activity analysis. Metabolism was affected by drought stress through clear segregation between control and stressed groups. More importantly, metabolism changed through time, gradually from 7 to 14 days. The pathways most affected by drought stress were: starch and sucrose metabolism, glyoxylate and dicarboxylate metabolism, alanine, aspartate and glutamate metabolism, arginine and proline metabolism, and glycine, serine and threonine metabolism. The analysis of the metabolic profile were efficient to correlate and differentiate groups of oil palm plants submitted to different levels of drought stress. Putative compounds and their affected pathways can be used in future multiomics analysis.
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spelling pubmed-84406122021-09-15 Metabolic effect of drought stress on the leaves of young oil palm (Elaeis guineensis) plants using UHPLC–MS and multivariate analysis Neto, Jorge Candido Rodrigues Vieira, Letícia Rios de Aquino Ribeiro, José Antônio de Sousa, Carlos Antônio Ferreira Júnior, Manoel Teixeira Souza Abdelnur, Patrícia Verardi Sci Rep Article The expansion of the oil palm in marginal areas can face challenges, such as water deficit, leading to an impact on palm oil production. A better understanding of the biological consequences of abiotic stresses on this crop can result from joint metabolic profiling and multivariate analysis. Metabolic profiling of leaves was performed from control and stressed plants (7 and 14 days of stress). Samples were extracted and analyzed on a UHPLC-ESI-Q-TOF-HRMS system. Acquired data were processed using XCMS Online and MetaboAnalyst for multivariate and pathway activity analysis. Metabolism was affected by drought stress through clear segregation between control and stressed groups. More importantly, metabolism changed through time, gradually from 7 to 14 days. The pathways most affected by drought stress were: starch and sucrose metabolism, glyoxylate and dicarboxylate metabolism, alanine, aspartate and glutamate metabolism, arginine and proline metabolism, and glycine, serine and threonine metabolism. The analysis of the metabolic profile were efficient to correlate and differentiate groups of oil palm plants submitted to different levels of drought stress. Putative compounds and their affected pathways can be used in future multiomics analysis. Nature Publishing Group UK 2021-09-14 /pmc/articles/PMC8440612/ /pubmed/34521943 http://dx.doi.org/10.1038/s41598-021-97835-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Neto, Jorge Candido Rodrigues
Vieira, Letícia Rios
de Aquino Ribeiro, José Antônio
de Sousa, Carlos Antônio Ferreira
Júnior, Manoel Teixeira Souza
Abdelnur, Patrícia Verardi
Metabolic effect of drought stress on the leaves of young oil palm (Elaeis guineensis) plants using UHPLC–MS and multivariate analysis
title Metabolic effect of drought stress on the leaves of young oil palm (Elaeis guineensis) plants using UHPLC–MS and multivariate analysis
title_full Metabolic effect of drought stress on the leaves of young oil palm (Elaeis guineensis) plants using UHPLC–MS and multivariate analysis
title_fullStr Metabolic effect of drought stress on the leaves of young oil palm (Elaeis guineensis) plants using UHPLC–MS and multivariate analysis
title_full_unstemmed Metabolic effect of drought stress on the leaves of young oil palm (Elaeis guineensis) plants using UHPLC–MS and multivariate analysis
title_short Metabolic effect of drought stress on the leaves of young oil palm (Elaeis guineensis) plants using UHPLC–MS and multivariate analysis
title_sort metabolic effect of drought stress on the leaves of young oil palm (elaeis guineensis) plants using uhplc–ms and multivariate analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440612/
https://www.ncbi.nlm.nih.gov/pubmed/34521943
http://dx.doi.org/10.1038/s41598-021-97835-x
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