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Advances in Understanding Leishmania Pathobiology: What Does RNA-Seq Tell Us?

Leishmaniasis is a vector-borne disease caused by a protozoa parasite from over 20 Leishmania species. The clinical manifestations and the outcome of the disease vary greatly. Global RNA sequencing (RNA-Seq) analyses emerged as a powerful technique to profile the changes in the transcriptome that oc...

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Autores principales: Salloum, Tamara, Tokajian, Sima, Hirt, Robert P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440825/
https://www.ncbi.nlm.nih.gov/pubmed/34540827
http://dx.doi.org/10.3389/fcell.2021.702240
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author Salloum, Tamara
Tokajian, Sima
Hirt, Robert P.
author_facet Salloum, Tamara
Tokajian, Sima
Hirt, Robert P.
author_sort Salloum, Tamara
collection PubMed
description Leishmaniasis is a vector-borne disease caused by a protozoa parasite from over 20 Leishmania species. The clinical manifestations and the outcome of the disease vary greatly. Global RNA sequencing (RNA-Seq) analyses emerged as a powerful technique to profile the changes in the transcriptome that occur in the Leishmania parasites and their infected host cells as the parasites progresses through their life cycle. Following the bite of a sandfly vector, Leishmania are transmitted to a mammalian host where neutrophils and macrophages are key cells mediating the interactions with the parasites and result in either the elimination the infection or contributing to its proliferation. This review focuses on RNA-Seq based transcriptomics analyses and summarizes the main findings derived from this technology. In doing so, we will highlight caveats in our understanding of the parasite’s pathobiology and suggest novel directions for research, including integrating more recent data highlighting the role of the bacterial members of the sandfly gut microbiota and the mammalian host skin microbiota in their potential role in influencing the quantitative and qualitative aspects of leishmaniasis pathology.
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spelling pubmed-84408252021-09-16 Advances in Understanding Leishmania Pathobiology: What Does RNA-Seq Tell Us? Salloum, Tamara Tokajian, Sima Hirt, Robert P. Front Cell Dev Biol Cell and Developmental Biology Leishmaniasis is a vector-borne disease caused by a protozoa parasite from over 20 Leishmania species. The clinical manifestations and the outcome of the disease vary greatly. Global RNA sequencing (RNA-Seq) analyses emerged as a powerful technique to profile the changes in the transcriptome that occur in the Leishmania parasites and their infected host cells as the parasites progresses through their life cycle. Following the bite of a sandfly vector, Leishmania are transmitted to a mammalian host where neutrophils and macrophages are key cells mediating the interactions with the parasites and result in either the elimination the infection or contributing to its proliferation. This review focuses on RNA-Seq based transcriptomics analyses and summarizes the main findings derived from this technology. In doing so, we will highlight caveats in our understanding of the parasite’s pathobiology and suggest novel directions for research, including integrating more recent data highlighting the role of the bacterial members of the sandfly gut microbiota and the mammalian host skin microbiota in their potential role in influencing the quantitative and qualitative aspects of leishmaniasis pathology. Frontiers Media S.A. 2021-09-01 /pmc/articles/PMC8440825/ /pubmed/34540827 http://dx.doi.org/10.3389/fcell.2021.702240 Text en Copyright © 2021 Salloum, Tokajian and Hirt. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Salloum, Tamara
Tokajian, Sima
Hirt, Robert P.
Advances in Understanding Leishmania Pathobiology: What Does RNA-Seq Tell Us?
title Advances in Understanding Leishmania Pathobiology: What Does RNA-Seq Tell Us?
title_full Advances in Understanding Leishmania Pathobiology: What Does RNA-Seq Tell Us?
title_fullStr Advances in Understanding Leishmania Pathobiology: What Does RNA-Seq Tell Us?
title_full_unstemmed Advances in Understanding Leishmania Pathobiology: What Does RNA-Seq Tell Us?
title_short Advances in Understanding Leishmania Pathobiology: What Does RNA-Seq Tell Us?
title_sort advances in understanding leishmania pathobiology: what does rna-seq tell us?
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440825/
https://www.ncbi.nlm.nih.gov/pubmed/34540827
http://dx.doi.org/10.3389/fcell.2021.702240
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