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Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm

Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis in small ruminants, a chronic disease characterized by the development of granulomas in superficial and visceral lymph nodes as well as in several organs. An important characteristic of the infection with this bacte...

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Autores principales: Santos, Laerte Marlon, Rodrigues, Daniela Méria, Kalil, Maurício Alcantara, Azevedo, Vasco, Meyer, Roberto, Umsza-Guez, Marcelo Andres, Machado, Bruna Aparecida, Seyffert, Nubia, Portela, Ricardo Wagner
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440938/
https://www.ncbi.nlm.nih.gov/pubmed/34540932
http://dx.doi.org/10.3389/fvets.2021.700030
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author Santos, Laerte Marlon
Rodrigues, Daniela Méria
Kalil, Maurício Alcantara
Azevedo, Vasco
Meyer, Roberto
Umsza-Guez, Marcelo Andres
Machado, Bruna Aparecida
Seyffert, Nubia
Portela, Ricardo Wagner
author_facet Santos, Laerte Marlon
Rodrigues, Daniela Méria
Kalil, Maurício Alcantara
Azevedo, Vasco
Meyer, Roberto
Umsza-Guez, Marcelo Andres
Machado, Bruna Aparecida
Seyffert, Nubia
Portela, Ricardo Wagner
author_sort Santos, Laerte Marlon
collection PubMed
description Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis in small ruminants, a chronic disease characterized by the development of granulomas in superficial and visceral lymph nodes as well as in several organs. An important characteristic of the infection with this bacterium is the formation of a biofilm and the absence of effective antibiotic therapy against the disease. From this scenario, the objective of this study was to evaluate the susceptibility of C. pseudotuberculosis to conventional antibiotics and to red, green, and brown propolis extracts obtained by the supercritical and ethanolic extraction methods as well as its activity in the bacterial biofilm. The results of the sensitivity test using antibiotics indicated a sensitivity of C. pseudotuberculosis strains to the antimicrobial agents. The ethanolic extract of green propolis and the supercritical red propolis extract showed the best antibacterial activities against planktonic C. pseudotuberculosis. A lower antimicrobial activity of the brown propolis extract was identified. Propolis extracts were effective in interfering with the formation of the C. pseudotuberculosis biofilm but had little activity on the consolidated biofilm. In conclusion, propolis extracts are more effective against C. pseudotuberculosis in the planktonic stage, being able to interfere with the formation of bacterial biofilm. However, the action of propolis extracts in a sessile and structured microbial biofilm is reduced.
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spelling pubmed-84409382021-09-16 Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm Santos, Laerte Marlon Rodrigues, Daniela Méria Kalil, Maurício Alcantara Azevedo, Vasco Meyer, Roberto Umsza-Guez, Marcelo Andres Machado, Bruna Aparecida Seyffert, Nubia Portela, Ricardo Wagner Front Vet Sci Veterinary Science Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis in small ruminants, a chronic disease characterized by the development of granulomas in superficial and visceral lymph nodes as well as in several organs. An important characteristic of the infection with this bacterium is the formation of a biofilm and the absence of effective antibiotic therapy against the disease. From this scenario, the objective of this study was to evaluate the susceptibility of C. pseudotuberculosis to conventional antibiotics and to red, green, and brown propolis extracts obtained by the supercritical and ethanolic extraction methods as well as its activity in the bacterial biofilm. The results of the sensitivity test using antibiotics indicated a sensitivity of C. pseudotuberculosis strains to the antimicrobial agents. The ethanolic extract of green propolis and the supercritical red propolis extract showed the best antibacterial activities against planktonic C. pseudotuberculosis. A lower antimicrobial activity of the brown propolis extract was identified. Propolis extracts were effective in interfering with the formation of the C. pseudotuberculosis biofilm but had little activity on the consolidated biofilm. In conclusion, propolis extracts are more effective against C. pseudotuberculosis in the planktonic stage, being able to interfere with the formation of bacterial biofilm. However, the action of propolis extracts in a sessile and structured microbial biofilm is reduced. Frontiers Media S.A. 2021-09-01 /pmc/articles/PMC8440938/ /pubmed/34540932 http://dx.doi.org/10.3389/fvets.2021.700030 Text en Copyright © 2021 Santos, Rodrigues, Kalil, Azevedo, Meyer, Umsza-Guez, Machado, Seyffert and Portela. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Santos, Laerte Marlon
Rodrigues, Daniela Méria
Kalil, Maurício Alcantara
Azevedo, Vasco
Meyer, Roberto
Umsza-Guez, Marcelo Andres
Machado, Bruna Aparecida
Seyffert, Nubia
Portela, Ricardo Wagner
Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm
title Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm
title_full Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm
title_fullStr Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm
title_full_unstemmed Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm
title_short Activity of Ethanolic and Supercritical Propolis Extracts in Corynebacterium pseudotuberculosis and Its Associated Biofilm
title_sort activity of ethanolic and supercritical propolis extracts in corynebacterium pseudotuberculosis and its associated biofilm
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8440938/
https://www.ncbi.nlm.nih.gov/pubmed/34540932
http://dx.doi.org/10.3389/fvets.2021.700030
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