Cargando…

Indigenous fungi from corn as a potential plant growth promoter and its role in Fusarium verticillioides suppression on corn

Indigenous fungi can suppress infection by pathogens and produce secondary metabolites that directly or indirectly affect plant growth. This study aimed to test indigenous fungi collected from corn plants as biological control agents and their effects on the viability and vigor of corn seeds. Purpos...

Descripción completa

Detalles Bibliográficos
Autores principales: Mirsam, Hishar, Kalqutny, Septian Hary, Suriani, Aqil, Muhammad, Azrai, Muhammad, Pakki, Syahrir, Muis, Amran, Djaenuddin, Nurasiah, Rauf, Abdul Wahid, Muslimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441163/
https://www.ncbi.nlm.nih.gov/pubmed/34553081
http://dx.doi.org/10.1016/j.heliyon.2021.e07926
_version_ 1783752820584873984
author Mirsam, Hishar
Kalqutny, Septian Hary
Suriani
Aqil, Muhammad
Azrai, Muhammad
Pakki, Syahrir
Muis, Amran
Djaenuddin, Nurasiah
Rauf, Abdul Wahid
Muslimin
author_facet Mirsam, Hishar
Kalqutny, Septian Hary
Suriani
Aqil, Muhammad
Azrai, Muhammad
Pakki, Syahrir
Muis, Amran
Djaenuddin, Nurasiah
Rauf, Abdul Wahid
Muslimin
author_sort Mirsam, Hishar
collection PubMed
description Indigenous fungi can suppress infection by pathogens and produce secondary metabolites that directly or indirectly affect plant growth. This study aimed to test indigenous fungi collected from corn plants as biological control agents and their effects on the viability and vigor of corn seeds. Purposive sampling method was used for sampling where soil samples taken from the rhizosphere zone, corn stem and leaf tissue from three locations namely Maros-South Sulawesi, Bone-South Sulawesi, Sigi-Central Sulawesi, Indonesia. Rhizospheric fungi were isolated from soil collected at the rhizosphere and rhizoplane using a serial dilution technique, while the endophytic fungi isolated from the leaves and stem tissues using surface sterilization method. The isolated fungi were cultured on a potato dextrose agar (PDA) medium. An antagonism test was performed using the dual culture method on PDA media with F. verticillioides as target pathogen. Pathogenicity test and the effect of fungi on corn seed germination was carried out using the blotter test method. Parameters observed were; necrotic symptoms on seedlings, growth potential, germination, growth rate, growth simultaneity, vigor index, germination rate, and time needed for 50% of the total germination. The effect of the isolated indigenous fungi on corn growth was carried out in-planta using seedling trays. The results of the blotter test and in-planta test were further confirmed by a physiological characteristic test. And assessing the fungi's ability to dissolve potassium, phosphate, and produce protease enzymes. A total of 89 fungal isolates were isolated and collected from various parts of the corn plant. Nineteen of the 89 fungal isolates showed inhibitory activity against F. verticillioides by ≥ 50% inhibition. The fungal isolates JRP 5 MRS, JRP 9 MRS, JRP 10 MRS, JRP 7 MRS, and JEDF 1B BN were selected based on the tests and showed a consistently positive effect on seed viability and vigor with a value of ≥90%. The isolates did not cause necrosis in corn, and had the ability to suppress the growth of pathogenic F. verticillioides by ≥ 50%.
format Online
Article
Text
id pubmed-8441163
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-84411632021-09-21 Indigenous fungi from corn as a potential plant growth promoter and its role in Fusarium verticillioides suppression on corn Mirsam, Hishar Kalqutny, Septian Hary Suriani Aqil, Muhammad Azrai, Muhammad Pakki, Syahrir Muis, Amran Djaenuddin, Nurasiah Rauf, Abdul Wahid Muslimin Heliyon Research Article Indigenous fungi can suppress infection by pathogens and produce secondary metabolites that directly or indirectly affect plant growth. This study aimed to test indigenous fungi collected from corn plants as biological control agents and their effects on the viability and vigor of corn seeds. Purposive sampling method was used for sampling where soil samples taken from the rhizosphere zone, corn stem and leaf tissue from three locations namely Maros-South Sulawesi, Bone-South Sulawesi, Sigi-Central Sulawesi, Indonesia. Rhizospheric fungi were isolated from soil collected at the rhizosphere and rhizoplane using a serial dilution technique, while the endophytic fungi isolated from the leaves and stem tissues using surface sterilization method. The isolated fungi were cultured on a potato dextrose agar (PDA) medium. An antagonism test was performed using the dual culture method on PDA media with F. verticillioides as target pathogen. Pathogenicity test and the effect of fungi on corn seed germination was carried out using the blotter test method. Parameters observed were; necrotic symptoms on seedlings, growth potential, germination, growth rate, growth simultaneity, vigor index, germination rate, and time needed for 50% of the total germination. The effect of the isolated indigenous fungi on corn growth was carried out in-planta using seedling trays. The results of the blotter test and in-planta test were further confirmed by a physiological characteristic test. And assessing the fungi's ability to dissolve potassium, phosphate, and produce protease enzymes. A total of 89 fungal isolates were isolated and collected from various parts of the corn plant. Nineteen of the 89 fungal isolates showed inhibitory activity against F. verticillioides by ≥ 50% inhibition. The fungal isolates JRP 5 MRS, JRP 9 MRS, JRP 10 MRS, JRP 7 MRS, and JEDF 1B BN were selected based on the tests and showed a consistently positive effect on seed viability and vigor with a value of ≥90%. The isolates did not cause necrosis in corn, and had the ability to suppress the growth of pathogenic F. verticillioides by ≥ 50%. Elsevier 2021-09-03 /pmc/articles/PMC8441163/ /pubmed/34553081 http://dx.doi.org/10.1016/j.heliyon.2021.e07926 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Mirsam, Hishar
Kalqutny, Septian Hary
Suriani
Aqil, Muhammad
Azrai, Muhammad
Pakki, Syahrir
Muis, Amran
Djaenuddin, Nurasiah
Rauf, Abdul Wahid
Muslimin
Indigenous fungi from corn as a potential plant growth promoter and its role in Fusarium verticillioides suppression on corn
title Indigenous fungi from corn as a potential plant growth promoter and its role in Fusarium verticillioides suppression on corn
title_full Indigenous fungi from corn as a potential plant growth promoter and its role in Fusarium verticillioides suppression on corn
title_fullStr Indigenous fungi from corn as a potential plant growth promoter and its role in Fusarium verticillioides suppression on corn
title_full_unstemmed Indigenous fungi from corn as a potential plant growth promoter and its role in Fusarium verticillioides suppression on corn
title_short Indigenous fungi from corn as a potential plant growth promoter and its role in Fusarium verticillioides suppression on corn
title_sort indigenous fungi from corn as a potential plant growth promoter and its role in fusarium verticillioides suppression on corn
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8441163/
https://www.ncbi.nlm.nih.gov/pubmed/34553081
http://dx.doi.org/10.1016/j.heliyon.2021.e07926
work_keys_str_mv AT mirsamhishar indigenousfungifromcornasapotentialplantgrowthpromoteranditsroleinfusariumverticillioidessuppressiononcorn
AT kalqutnyseptianhary indigenousfungifromcornasapotentialplantgrowthpromoteranditsroleinfusariumverticillioidessuppressiononcorn
AT suriani indigenousfungifromcornasapotentialplantgrowthpromoteranditsroleinfusariumverticillioidessuppressiononcorn
AT aqilmuhammad indigenousfungifromcornasapotentialplantgrowthpromoteranditsroleinfusariumverticillioidessuppressiononcorn
AT azraimuhammad indigenousfungifromcornasapotentialplantgrowthpromoteranditsroleinfusariumverticillioidessuppressiononcorn
AT pakkisyahrir indigenousfungifromcornasapotentialplantgrowthpromoteranditsroleinfusariumverticillioidessuppressiononcorn
AT muisamran indigenousfungifromcornasapotentialplantgrowthpromoteranditsroleinfusariumverticillioidessuppressiononcorn
AT djaenuddinnurasiah indigenousfungifromcornasapotentialplantgrowthpromoteranditsroleinfusariumverticillioidessuppressiononcorn
AT raufabdulwahid indigenousfungifromcornasapotentialplantgrowthpromoteranditsroleinfusariumverticillioidessuppressiononcorn
AT muslimin indigenousfungifromcornasapotentialplantgrowthpromoteranditsroleinfusariumverticillioidessuppressiononcorn