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maxRatio improves the detection of samples with abnormal amplification profiles on QIAgen’s artus HIV-1 qPCR assay

Background: Accurate viral load (VL) determination is paramount to determine the efficacy of anti-HIV-1 therapy. The conventional method used, fit-point (FP), assumes an equal efficiency in the polymerase chain reaction (PCR) among samples that might not hold for low-input templates. An alternative...

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Detalles Bibliográficos
Autores principales: Marongiu, Luigi, Shain, Eric B., Martinelli, Marianna, Pagliari, Matteo, Allgayer, Heike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8442116/
https://www.ncbi.nlm.nih.gov/pubmed/34557295
http://dx.doi.org/10.12688/f1000research.25738.3