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Integrative analysis of ferroptosis-related genes in ulcerative colitis

OBJECTIVE: The aim of this study was to identify and validate ferroptosis-related markers in ulcerative colitis (UC) to explore new directions for UC diagnosis and treatment. METHODS: We screened UC chips and ferroptosis-related genes from the Gene Expression Omnibus (GEO), FerrDb, and GeneCards dat...

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Autores principales: Cui, De-jun, Chen, Chen, Yuan, Wen-qiang, Yang, Yun-han, Han, Lu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8442491/
https://www.ncbi.nlm.nih.gov/pubmed/34510961
http://dx.doi.org/10.1177/03000605211042975
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author Cui, De-jun
Chen, Chen
Yuan, Wen-qiang
Yang, Yun-han
Han, Lu
author_facet Cui, De-jun
Chen, Chen
Yuan, Wen-qiang
Yang, Yun-han
Han, Lu
author_sort Cui, De-jun
collection PubMed
description OBJECTIVE: The aim of this study was to identify and validate ferroptosis-related markers in ulcerative colitis (UC) to explore new directions for UC diagnosis and treatment. METHODS: We screened UC chips and ferroptosis-related genes from the Gene Expression Omnibus (GEO), FerrDb, and GeneCards databases. The differentially expressed genes (DEGs) and ferroptosis-related DEGs between the UC group and normal controls were analyzed using bioinformatics methods. Enrichment analysis, protein–protein interaction analysis, and hub genes were screened. Peripheral blood chip and animal experiments were used to validate the ferroptosis-related hub genes. Finally, hub gene–transcription factor, hub gene–microRNA (miRNA), and hub gene–drug interaction networks were constructed. RESULTS: Overall, 26 ferroptosis-related DEGs were identified that were significantly enriched in energy pathways and metabolism. We identified ten ferroptosis-related hub genes from the protein–protein interaction network: IL6, PTGS2, HIF1A, CD44, MUC1, CAV1, NOS2, CXCL2, SCD, and ACSL4. In the peripheral blood chip GSE94648, CD44 and MUC1 were upregulated, which was consistent with the expression trend in GSE75214. Animal experiments showed that CD44 expression was significantly increased in the colon. CONCLUSIONS: Our findings indicate that CD44 and MUC1 may be ferroptosis-related markers in UC.
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spelling pubmed-84424912021-09-16 Integrative analysis of ferroptosis-related genes in ulcerative colitis Cui, De-jun Chen, Chen Yuan, Wen-qiang Yang, Yun-han Han, Lu J Int Med Res Pre-Clinical Research Report OBJECTIVE: The aim of this study was to identify and validate ferroptosis-related markers in ulcerative colitis (UC) to explore new directions for UC diagnosis and treatment. METHODS: We screened UC chips and ferroptosis-related genes from the Gene Expression Omnibus (GEO), FerrDb, and GeneCards databases. The differentially expressed genes (DEGs) and ferroptosis-related DEGs between the UC group and normal controls were analyzed using bioinformatics methods. Enrichment analysis, protein–protein interaction analysis, and hub genes were screened. Peripheral blood chip and animal experiments were used to validate the ferroptosis-related hub genes. Finally, hub gene–transcription factor, hub gene–microRNA (miRNA), and hub gene–drug interaction networks were constructed. RESULTS: Overall, 26 ferroptosis-related DEGs were identified that were significantly enriched in energy pathways and metabolism. We identified ten ferroptosis-related hub genes from the protein–protein interaction network: IL6, PTGS2, HIF1A, CD44, MUC1, CAV1, NOS2, CXCL2, SCD, and ACSL4. In the peripheral blood chip GSE94648, CD44 and MUC1 were upregulated, which was consistent with the expression trend in GSE75214. Animal experiments showed that CD44 expression was significantly increased in the colon. CONCLUSIONS: Our findings indicate that CD44 and MUC1 may be ferroptosis-related markers in UC. SAGE Publications 2021-09-12 /pmc/articles/PMC8442491/ /pubmed/34510961 http://dx.doi.org/10.1177/03000605211042975 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Pre-Clinical Research Report
Cui, De-jun
Chen, Chen
Yuan, Wen-qiang
Yang, Yun-han
Han, Lu
Integrative analysis of ferroptosis-related genes in ulcerative colitis
title Integrative analysis of ferroptosis-related genes in ulcerative colitis
title_full Integrative analysis of ferroptosis-related genes in ulcerative colitis
title_fullStr Integrative analysis of ferroptosis-related genes in ulcerative colitis
title_full_unstemmed Integrative analysis of ferroptosis-related genes in ulcerative colitis
title_short Integrative analysis of ferroptosis-related genes in ulcerative colitis
title_sort integrative analysis of ferroptosis-related genes in ulcerative colitis
topic Pre-Clinical Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8442491/
https://www.ncbi.nlm.nih.gov/pubmed/34510961
http://dx.doi.org/10.1177/03000605211042975
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