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Development of non-transgenic glyphosate tolerant wheat by TILLING
Glyphosate (N-phosphonomethyl-glycine) is the world’s most widely used broad spectrum, post-emergence herbicide. It inhibits the chloroplast-targeted enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19), a component of the plant and microorganism-specific shikimate pathway and a k...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443057/ https://www.ncbi.nlm.nih.gov/pubmed/34525118 http://dx.doi.org/10.1371/journal.pone.0245802 |
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author | Moehs, Charles P. Austill, William J. Facciotti, Daniel Holm, Aaron Loeffler, Dayna Lu, Zhongjin Mullenberg, Jessica C. Slade, Ann J. Steine, Michael N. van Boxtel, Jos McGuire, Cate |
author_facet | Moehs, Charles P. Austill, William J. Facciotti, Daniel Holm, Aaron Loeffler, Dayna Lu, Zhongjin Mullenberg, Jessica C. Slade, Ann J. Steine, Michael N. van Boxtel, Jos McGuire, Cate |
author_sort | Moehs, Charles P. |
collection | PubMed |
description | Glyphosate (N-phosphonomethyl-glycine) is the world’s most widely used broad spectrum, post-emergence herbicide. It inhibits the chloroplast-targeted enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19), a component of the plant and microorganism-specific shikimate pathway and a key catalyst in the production of aromatic amino acids. Variants of EPSPS that are not inhibited by glyphosate due to particular amino acid alterations in the active site of the enzyme are known. Some of these variants have been identified in weed species that have developed resistance to glyphosate because of the strong selective pressure of continuous, heavy glyphosate use. We have used TILLING (Targeting Induced Local Lesions in Genomes), a non-transgenic, target-selected, reverse genetics, mutation breeding technique, and conventional genetic crosses, to identify and combine, through two rounds of mutagenesis, wheat lines having both T(102)I and P(106)S (so-called TIPS enzyme) mutations in both the A and the D sub-genome homoeologous copies of the wheat EPSPS gene. The combined effects of the T(102)I and P(106)S mutations are known from previous work in multiple species to minimize the binding of the herbicide while maintaining the affinity of the catalytic site for its native substrates. These novel wheat lines exhibit substantial tolerance to commercially relevant levels of glyphosate. |
format | Online Article Text |
id | pubmed-8443057 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-84430572021-09-16 Development of non-transgenic glyphosate tolerant wheat by TILLING Moehs, Charles P. Austill, William J. Facciotti, Daniel Holm, Aaron Loeffler, Dayna Lu, Zhongjin Mullenberg, Jessica C. Slade, Ann J. Steine, Michael N. van Boxtel, Jos McGuire, Cate PLoS One Research Article Glyphosate (N-phosphonomethyl-glycine) is the world’s most widely used broad spectrum, post-emergence herbicide. It inhibits the chloroplast-targeted enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS; EC 2.5.1.19), a component of the plant and microorganism-specific shikimate pathway and a key catalyst in the production of aromatic amino acids. Variants of EPSPS that are not inhibited by glyphosate due to particular amino acid alterations in the active site of the enzyme are known. Some of these variants have been identified in weed species that have developed resistance to glyphosate because of the strong selective pressure of continuous, heavy glyphosate use. We have used TILLING (Targeting Induced Local Lesions in Genomes), a non-transgenic, target-selected, reverse genetics, mutation breeding technique, and conventional genetic crosses, to identify and combine, through two rounds of mutagenesis, wheat lines having both T(102)I and P(106)S (so-called TIPS enzyme) mutations in both the A and the D sub-genome homoeologous copies of the wheat EPSPS gene. The combined effects of the T(102)I and P(106)S mutations are known from previous work in multiple species to minimize the binding of the herbicide while maintaining the affinity of the catalytic site for its native substrates. These novel wheat lines exhibit substantial tolerance to commercially relevant levels of glyphosate. Public Library of Science 2021-09-15 /pmc/articles/PMC8443057/ /pubmed/34525118 http://dx.doi.org/10.1371/journal.pone.0245802 Text en © 2021 Moehs et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Moehs, Charles P. Austill, William J. Facciotti, Daniel Holm, Aaron Loeffler, Dayna Lu, Zhongjin Mullenberg, Jessica C. Slade, Ann J. Steine, Michael N. van Boxtel, Jos McGuire, Cate Development of non-transgenic glyphosate tolerant wheat by TILLING |
title | Development of non-transgenic glyphosate tolerant wheat by TILLING |
title_full | Development of non-transgenic glyphosate tolerant wheat by TILLING |
title_fullStr | Development of non-transgenic glyphosate tolerant wheat by TILLING |
title_full_unstemmed | Development of non-transgenic glyphosate tolerant wheat by TILLING |
title_short | Development of non-transgenic glyphosate tolerant wheat by TILLING |
title_sort | development of non-transgenic glyphosate tolerant wheat by tilling |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443057/ https://www.ncbi.nlm.nih.gov/pubmed/34525118 http://dx.doi.org/10.1371/journal.pone.0245802 |
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