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The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei
The biosynthesis of ribosomes is a complex cellular process involving ribosomal RNA, ribosomal proteins and several further trans-acting factors. DExD/H box proteins constitute the largest family of trans-acting protein factors involved in this process. Several members of this protein family have be...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443567/ https://www.ncbi.nlm.nih.gov/pubmed/34526538 http://dx.doi.org/10.1038/s41598-021-97020-0 |
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author | Bakari-Soale, Majeed Ikenga, Nonso Josephat Scheibe, Marion Butter, Falk Jones, Nicola G. Kramer, Susanne Engstler, Markus |
author_facet | Bakari-Soale, Majeed Ikenga, Nonso Josephat Scheibe, Marion Butter, Falk Jones, Nicola G. Kramer, Susanne Engstler, Markus |
author_sort | Bakari-Soale, Majeed |
collection | PubMed |
description | The biosynthesis of ribosomes is a complex cellular process involving ribosomal RNA, ribosomal proteins and several further trans-acting factors. DExD/H box proteins constitute the largest family of trans-acting protein factors involved in this process. Several members of this protein family have been directly implicated in ribosome biogenesis in yeast. In trypanosomes, ribosome biogenesis differs in several features from the process described in yeast. Here, we have identified the DExD/H box helicase Hel66 as being involved in ribosome biogenesis. The protein is unique to Kinetoplastida, localises to the nucleolus and its depletion via RNAi caused a severe growth defect. Loss of the protein resulted in a decrease of global translation and accumulation of rRNA processing intermediates for both the small and large ribosomal subunits. Only a few factors involved in trypanosome rRNA biogenesis have been described so far and our findings contribute to gaining a more comprehensive picture of this essential process. |
format | Online Article Text |
id | pubmed-8443567 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84435672021-09-20 The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei Bakari-Soale, Majeed Ikenga, Nonso Josephat Scheibe, Marion Butter, Falk Jones, Nicola G. Kramer, Susanne Engstler, Markus Sci Rep Article The biosynthesis of ribosomes is a complex cellular process involving ribosomal RNA, ribosomal proteins and several further trans-acting factors. DExD/H box proteins constitute the largest family of trans-acting protein factors involved in this process. Several members of this protein family have been directly implicated in ribosome biogenesis in yeast. In trypanosomes, ribosome biogenesis differs in several features from the process described in yeast. Here, we have identified the DExD/H box helicase Hel66 as being involved in ribosome biogenesis. The protein is unique to Kinetoplastida, localises to the nucleolus and its depletion via RNAi caused a severe growth defect. Loss of the protein resulted in a decrease of global translation and accumulation of rRNA processing intermediates for both the small and large ribosomal subunits. Only a few factors involved in trypanosome rRNA biogenesis have been described so far and our findings contribute to gaining a more comprehensive picture of this essential process. Nature Publishing Group UK 2021-09-15 /pmc/articles/PMC8443567/ /pubmed/34526538 http://dx.doi.org/10.1038/s41598-021-97020-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Bakari-Soale, Majeed Ikenga, Nonso Josephat Scheibe, Marion Butter, Falk Jones, Nicola G. Kramer, Susanne Engstler, Markus The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei |
title | The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei |
title_full | The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei |
title_fullStr | The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei |
title_full_unstemmed | The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei |
title_short | The nucleolar DExD/H protein Hel66 is involved in ribosome biogenesis in Trypanosoma brucei |
title_sort | nucleolar dexd/h protein hel66 is involved in ribosome biogenesis in trypanosoma brucei |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443567/ https://www.ncbi.nlm.nih.gov/pubmed/34526538 http://dx.doi.org/10.1038/s41598-021-97020-0 |
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