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Development of the first axillary in vitro shoot multiplication protocol for coconut palms
The coconut palm or “tree of life” is one of nature’s most useful plants and the demand for its fruit is increasing. However, coconut production is threatened by ageing plantations, pests and diseases. Currently, the palm is exclusively propagated via seeds, limiting the amount of planting material....
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443624/ https://www.ncbi.nlm.nih.gov/pubmed/34526563 http://dx.doi.org/10.1038/s41598-021-97718-1 |
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author | Wilms, Hannes De Bièvre, Dries Longin, Kevin Swennen, Rony Rhee, Juhee Panis, Bart |
author_facet | Wilms, Hannes De Bièvre, Dries Longin, Kevin Swennen, Rony Rhee, Juhee Panis, Bart |
author_sort | Wilms, Hannes |
collection | PubMed |
description | The coconut palm or “tree of life” is one of nature’s most useful plants and the demand for its fruit is increasing. However, coconut production is threatened by ageing plantations, pests and diseases. Currently, the palm is exclusively propagated via seeds, limiting the amount of planting material. A novel micropropagation method is presented, based on axillary shoot formation. Apical meristems of in vitro coconut seedlings are cultured onto Y3 medium containing 1 µM TDZ. This induces the apical meristem to proliferate through axillary shoots in ~ 27% of the initiated explants. These axillary shoots are seen as white clumps of proliferating tissue and can be multiplied at a large scale or regenerated into rooted in vitro plantlets. This innovative micropropagation method will enable the production of disease-free, high quality in vitro plantlets, which will solve the worldwide scarcity of coconut planting material. |
format | Online Article Text |
id | pubmed-8443624 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84436242021-09-20 Development of the first axillary in vitro shoot multiplication protocol for coconut palms Wilms, Hannes De Bièvre, Dries Longin, Kevin Swennen, Rony Rhee, Juhee Panis, Bart Sci Rep Article The coconut palm or “tree of life” is one of nature’s most useful plants and the demand for its fruit is increasing. However, coconut production is threatened by ageing plantations, pests and diseases. Currently, the palm is exclusively propagated via seeds, limiting the amount of planting material. A novel micropropagation method is presented, based on axillary shoot formation. Apical meristems of in vitro coconut seedlings are cultured onto Y3 medium containing 1 µM TDZ. This induces the apical meristem to proliferate through axillary shoots in ~ 27% of the initiated explants. These axillary shoots are seen as white clumps of proliferating tissue and can be multiplied at a large scale or regenerated into rooted in vitro plantlets. This innovative micropropagation method will enable the production of disease-free, high quality in vitro plantlets, which will solve the worldwide scarcity of coconut planting material. Nature Publishing Group UK 2021-09-15 /pmc/articles/PMC8443624/ /pubmed/34526563 http://dx.doi.org/10.1038/s41598-021-97718-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Wilms, Hannes De Bièvre, Dries Longin, Kevin Swennen, Rony Rhee, Juhee Panis, Bart Development of the first axillary in vitro shoot multiplication protocol for coconut palms |
title | Development of the first axillary in vitro shoot multiplication protocol for coconut palms |
title_full | Development of the first axillary in vitro shoot multiplication protocol for coconut palms |
title_fullStr | Development of the first axillary in vitro shoot multiplication protocol for coconut palms |
title_full_unstemmed | Development of the first axillary in vitro shoot multiplication protocol for coconut palms |
title_short | Development of the first axillary in vitro shoot multiplication protocol for coconut palms |
title_sort | development of the first axillary in vitro shoot multiplication protocol for coconut palms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443624/ https://www.ncbi.nlm.nih.gov/pubmed/34526563 http://dx.doi.org/10.1038/s41598-021-97718-1 |
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