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Rheotaxis-based sperm separation using a biomimicry microfluidic device

Sperm selection is crucial to assisted reproduction, influencing the success rate of the treatment cycle and offspring health. However, in the current clinical sperm selection practices, bypassing almost all the natural selection barriers is a major concern. Here, we present a biomimicry microfluidi...

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Autores principales: Sarbandi, Iman Ramazani, Lesani, Ali, Moghimi Zand, Mahdi, Nosrati, Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443654/
https://www.ncbi.nlm.nih.gov/pubmed/34526568
http://dx.doi.org/10.1038/s41598-021-97602-y
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author Sarbandi, Iman Ramazani
Lesani, Ali
Moghimi Zand, Mahdi
Nosrati, Reza
author_facet Sarbandi, Iman Ramazani
Lesani, Ali
Moghimi Zand, Mahdi
Nosrati, Reza
author_sort Sarbandi, Iman Ramazani
collection PubMed
description Sperm selection is crucial to assisted reproduction, influencing the success rate of the treatment cycle and offspring health. However, in the current clinical sperm selection practices, bypassing almost all the natural selection barriers is a major concern. Here, we present a biomimicry microfluidic method, inspired by the anatomy of the female reproductive tract, that separates motile sperm based on their rheotaxis behavior to swim against the flow into low shear rate regions. The device includes micropocket geometries that recall the oval-shaped microstructures of the female fallopian tube to create shear protected zones for sperm separation. Clinical tests with human samples indicate that the device is capable of isolating viable and highly motile sperm based on their rheotaxis responses, resulting in a separation efficiency of 100%. The device presents an automated alternative for the current sperm selection practices in assisted reproduction.
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spelling pubmed-84436542021-09-20 Rheotaxis-based sperm separation using a biomimicry microfluidic device Sarbandi, Iman Ramazani Lesani, Ali Moghimi Zand, Mahdi Nosrati, Reza Sci Rep Article Sperm selection is crucial to assisted reproduction, influencing the success rate of the treatment cycle and offspring health. However, in the current clinical sperm selection practices, bypassing almost all the natural selection barriers is a major concern. Here, we present a biomimicry microfluidic method, inspired by the anatomy of the female reproductive tract, that separates motile sperm based on their rheotaxis behavior to swim against the flow into low shear rate regions. The device includes micropocket geometries that recall the oval-shaped microstructures of the female fallopian tube to create shear protected zones for sperm separation. Clinical tests with human samples indicate that the device is capable of isolating viable and highly motile sperm based on their rheotaxis responses, resulting in a separation efficiency of 100%. The device presents an automated alternative for the current sperm selection practices in assisted reproduction. Nature Publishing Group UK 2021-09-15 /pmc/articles/PMC8443654/ /pubmed/34526568 http://dx.doi.org/10.1038/s41598-021-97602-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Sarbandi, Iman Ramazani
Lesani, Ali
Moghimi Zand, Mahdi
Nosrati, Reza
Rheotaxis-based sperm separation using a biomimicry microfluidic device
title Rheotaxis-based sperm separation using a biomimicry microfluidic device
title_full Rheotaxis-based sperm separation using a biomimicry microfluidic device
title_fullStr Rheotaxis-based sperm separation using a biomimicry microfluidic device
title_full_unstemmed Rheotaxis-based sperm separation using a biomimicry microfluidic device
title_short Rheotaxis-based sperm separation using a biomimicry microfluidic device
title_sort rheotaxis-based sperm separation using a biomimicry microfluidic device
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443654/
https://www.ncbi.nlm.nih.gov/pubmed/34526568
http://dx.doi.org/10.1038/s41598-021-97602-y
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