A gain series method for accurate EMCCD calibration

Calibration of the gain and digital conversion factor of an EMCCD is necessary for accurate photon counting. We present a new method to quickly calibrate multiple gain settings of an EMCCD camera. Acquiring gain-series calibration data and analyzing the resulting images with the EMCCD noise model mo...

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Detalles Bibliográficos
Autores principales: Ryan, Duncan P., Dunlap, Megan K., Gelfand, Martin P., Werner, James H., Van Orden, Alan K., Goodwin, Peter M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443689/
https://www.ncbi.nlm.nih.gov/pubmed/34526588
http://dx.doi.org/10.1038/s41598-021-97759-6
Descripción
Sumario:Calibration of the gain and digital conversion factor of an EMCCD is necessary for accurate photon counting. We present a new method to quickly calibrate multiple gain settings of an EMCCD camera. Acquiring gain-series calibration data and analyzing the resulting images with the EMCCD noise model more accurately estimates the gain response of the camera. Furthermore, we develop a method to compare the results from different calibration approaches. Gain-series calibration outperforms all other methods in this self-consistency test.

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