Cargando…

Fluorescent Carbon Dots as Biosensor, Green Reductant, and Biomarker

[Image: see text] Carbon dots, the celebrated green material among the nanocarbon family, are blessed with several interesting features like biocompatibility, solubility, tunable luminescence, and so forth. Herein, carbon dots derived from Mint leaf extract (M-CDs) via a green method are exploited f...

Descripción completa

Detalles Bibliográficos
Autores principales: Raveendran, Varsha, Kizhakayil, Renuka Neeroli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8444300/
https://www.ncbi.nlm.nih.gov/pubmed/34549145
http://dx.doi.org/10.1021/acsomega.1c03481
_version_ 1784568461470466048
author Raveendran, Varsha
Kizhakayil, Renuka Neeroli
author_facet Raveendran, Varsha
Kizhakayil, Renuka Neeroli
author_sort Raveendran, Varsha
collection PubMed
description [Image: see text] Carbon dots, the celebrated green material among the nanocarbon family, are blessed with several interesting features like biocompatibility, solubility, tunable luminescence, and so forth. Herein, carbon dots derived from Mint leaf extract (M-CDs) via a green method are exploited for versatile applications as a biosensor, reductant, and biomarker. M-CDs are applied for fluorimetric sensing of biologically relevant folic acid through quenching response originating from the inner filter effect, with a limit of detection of 280 nM. The carbon dots were highly selective toward folic acid in a collection of 16 biomolecules. The specificity of carbon dots toward folic acid is explained based on the interaction between the two. Along with sensing, herein, we project M-CDs as a green reducing agent by demonstrating the reduction of Fe(III) and noble metal nanoparticle synthesis from their salt solutions. The particles are found to be significantly non-cytotoxic, as evident from the MTT assay performed on primary H8 cells. The application of M-CDs in multicolor imaging is also illustrated using HeLa cells.
format Online
Article
Text
id pubmed-8444300
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-84443002021-09-20 Fluorescent Carbon Dots as Biosensor, Green Reductant, and Biomarker Raveendran, Varsha Kizhakayil, Renuka Neeroli ACS Omega [Image: see text] Carbon dots, the celebrated green material among the nanocarbon family, are blessed with several interesting features like biocompatibility, solubility, tunable luminescence, and so forth. Herein, carbon dots derived from Mint leaf extract (M-CDs) via a green method are exploited for versatile applications as a biosensor, reductant, and biomarker. M-CDs are applied for fluorimetric sensing of biologically relevant folic acid through quenching response originating from the inner filter effect, with a limit of detection of 280 nM. The carbon dots were highly selective toward folic acid in a collection of 16 biomolecules. The specificity of carbon dots toward folic acid is explained based on the interaction between the two. Along with sensing, herein, we project M-CDs as a green reducing agent by demonstrating the reduction of Fe(III) and noble metal nanoparticle synthesis from their salt solutions. The particles are found to be significantly non-cytotoxic, as evident from the MTT assay performed on primary H8 cells. The application of M-CDs in multicolor imaging is also illustrated using HeLa cells. American Chemical Society 2021-08-27 /pmc/articles/PMC8444300/ /pubmed/34549145 http://dx.doi.org/10.1021/acsomega.1c03481 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Raveendran, Varsha
Kizhakayil, Renuka Neeroli
Fluorescent Carbon Dots as Biosensor, Green Reductant, and Biomarker
title Fluorescent Carbon Dots as Biosensor, Green Reductant, and Biomarker
title_full Fluorescent Carbon Dots as Biosensor, Green Reductant, and Biomarker
title_fullStr Fluorescent Carbon Dots as Biosensor, Green Reductant, and Biomarker
title_full_unstemmed Fluorescent Carbon Dots as Biosensor, Green Reductant, and Biomarker
title_short Fluorescent Carbon Dots as Biosensor, Green Reductant, and Biomarker
title_sort fluorescent carbon dots as biosensor, green reductant, and biomarker
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8444300/
https://www.ncbi.nlm.nih.gov/pubmed/34549145
http://dx.doi.org/10.1021/acsomega.1c03481
work_keys_str_mv AT raveendranvarsha fluorescentcarbondotsasbiosensorgreenreductantandbiomarker
AT kizhakayilrenukaneeroli fluorescentcarbondotsasbiosensorgreenreductantandbiomarker