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miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2

microRNA-342-3p plays an important role in tumor occurrence and development. However, the expression pattern and roles of microRNA-342-3p in nonsmall cell lung cancer remain poorly understood. In the current study, we explored the roles and underlying mechanisms of microRNA-342-3p in nonsmall cell l...

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Autores principales: Chen, Zhongjie, Ying, Junjie, Shang, Wenjun, Ding, Dongxiao, Guo, Min, Wang, Haifeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445541/
https://www.ncbi.nlm.nih.gov/pubmed/34520298
http://dx.doi.org/10.1177/15330338211041193
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author Chen, Zhongjie
Ying, Junjie
Shang, Wenjun
Ding, Dongxiao
Guo, Min
Wang, Haifeng
author_facet Chen, Zhongjie
Ying, Junjie
Shang, Wenjun
Ding, Dongxiao
Guo, Min
Wang, Haifeng
author_sort Chen, Zhongjie
collection PubMed
description microRNA-342-3p plays an important role in tumor occurrence and development. However, the expression pattern and roles of microRNA-342-3p in nonsmall cell lung cancer remain poorly understood. In the current study, we explored the roles and underlying mechanisms of microRNA-342-3p in nonsmall cell lung cancer via gain- and loss-of-function analyses. We used quantitative reverse-transcription-polymerase chain reaction and western blotting assays to measure the expression levels of microRNA-342-3p in nonsmall-cell lung cancer and B-cell lymphoma-2. Furthermore, we used small interfering RNA and RNA mimics to analyze the functions and underlying mechanisms of microRNA-342-3p in nonsmall cell lung cancer cells. A luciferase reporter assay was performed to evaluate the direct binding site of the 5′-untranslated region of B-cell lymphoma-2 targeted by microRNA-342-3p. We found that the expression of microRNA-342-3p was significantly lower in nonsmall cell lung cancer cells and tissues than in normal cells and tissues. The upregulation of microRNA-342-3p suppressed cell proliferation while promoting apoptosis in H1975, H460, and H226 cells. The overexpression of microRNA-342-3p in nonsmall cell lung cancer cells led to the downregulation of mRNA and protein levels in B-cell lymphoma-2 cells. Thus, B-cell lymphoma-2 was identified as a direct target of microRNA-342-3p. These findings indicate that microRNA-342-3p inhibits the growth of nonsmall cell lung cancer by repressing the expression of B-cell lymphoma-2, which suggests that microRNA-342-3p could be a potential target for the treatment of nonsmall cell lung cancer.
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spelling pubmed-84455412021-09-17 miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2 Chen, Zhongjie Ying, Junjie Shang, Wenjun Ding, Dongxiao Guo, Min Wang, Haifeng Technol Cancer Res Treat Original Article microRNA-342-3p plays an important role in tumor occurrence and development. However, the expression pattern and roles of microRNA-342-3p in nonsmall cell lung cancer remain poorly understood. In the current study, we explored the roles and underlying mechanisms of microRNA-342-3p in nonsmall cell lung cancer via gain- and loss-of-function analyses. We used quantitative reverse-transcription-polymerase chain reaction and western blotting assays to measure the expression levels of microRNA-342-3p in nonsmall-cell lung cancer and B-cell lymphoma-2. Furthermore, we used small interfering RNA and RNA mimics to analyze the functions and underlying mechanisms of microRNA-342-3p in nonsmall cell lung cancer cells. A luciferase reporter assay was performed to evaluate the direct binding site of the 5′-untranslated region of B-cell lymphoma-2 targeted by microRNA-342-3p. We found that the expression of microRNA-342-3p was significantly lower in nonsmall cell lung cancer cells and tissues than in normal cells and tissues. The upregulation of microRNA-342-3p suppressed cell proliferation while promoting apoptosis in H1975, H460, and H226 cells. The overexpression of microRNA-342-3p in nonsmall cell lung cancer cells led to the downregulation of mRNA and protein levels in B-cell lymphoma-2 cells. Thus, B-cell lymphoma-2 was identified as a direct target of microRNA-342-3p. These findings indicate that microRNA-342-3p inhibits the growth of nonsmall cell lung cancer by repressing the expression of B-cell lymphoma-2, which suggests that microRNA-342-3p could be a potential target for the treatment of nonsmall cell lung cancer. SAGE Publications 2021-09-14 /pmc/articles/PMC8445541/ /pubmed/34520298 http://dx.doi.org/10.1177/15330338211041193 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Chen, Zhongjie
Ying, Junjie
Shang, Wenjun
Ding, Dongxiao
Guo, Min
Wang, Haifeng
miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2
title miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2
title_full miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2
title_fullStr miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2
title_full_unstemmed miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2
title_short miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2
title_sort mir-342-3p regulates the proliferation and apoptosis of nsclc cells by targeting bcl-2
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445541/
https://www.ncbi.nlm.nih.gov/pubmed/34520298
http://dx.doi.org/10.1177/15330338211041193
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