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miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2
microRNA-342-3p plays an important role in tumor occurrence and development. However, the expression pattern and roles of microRNA-342-3p in nonsmall cell lung cancer remain poorly understood. In the current study, we explored the roles and underlying mechanisms of microRNA-342-3p in nonsmall cell l...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445541/ https://www.ncbi.nlm.nih.gov/pubmed/34520298 http://dx.doi.org/10.1177/15330338211041193 |
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author | Chen, Zhongjie Ying, Junjie Shang, Wenjun Ding, Dongxiao Guo, Min Wang, Haifeng |
author_facet | Chen, Zhongjie Ying, Junjie Shang, Wenjun Ding, Dongxiao Guo, Min Wang, Haifeng |
author_sort | Chen, Zhongjie |
collection | PubMed |
description | microRNA-342-3p plays an important role in tumor occurrence and development. However, the expression pattern and roles of microRNA-342-3p in nonsmall cell lung cancer remain poorly understood. In the current study, we explored the roles and underlying mechanisms of microRNA-342-3p in nonsmall cell lung cancer via gain- and loss-of-function analyses. We used quantitative reverse-transcription-polymerase chain reaction and western blotting assays to measure the expression levels of microRNA-342-3p in nonsmall-cell lung cancer and B-cell lymphoma-2. Furthermore, we used small interfering RNA and RNA mimics to analyze the functions and underlying mechanisms of microRNA-342-3p in nonsmall cell lung cancer cells. A luciferase reporter assay was performed to evaluate the direct binding site of the 5′-untranslated region of B-cell lymphoma-2 targeted by microRNA-342-3p. We found that the expression of microRNA-342-3p was significantly lower in nonsmall cell lung cancer cells and tissues than in normal cells and tissues. The upregulation of microRNA-342-3p suppressed cell proliferation while promoting apoptosis in H1975, H460, and H226 cells. The overexpression of microRNA-342-3p in nonsmall cell lung cancer cells led to the downregulation of mRNA and protein levels in B-cell lymphoma-2 cells. Thus, B-cell lymphoma-2 was identified as a direct target of microRNA-342-3p. These findings indicate that microRNA-342-3p inhibits the growth of nonsmall cell lung cancer by repressing the expression of B-cell lymphoma-2, which suggests that microRNA-342-3p could be a potential target for the treatment of nonsmall cell lung cancer. |
format | Online Article Text |
id | pubmed-8445541 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-84455412021-09-17 miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2 Chen, Zhongjie Ying, Junjie Shang, Wenjun Ding, Dongxiao Guo, Min Wang, Haifeng Technol Cancer Res Treat Original Article microRNA-342-3p plays an important role in tumor occurrence and development. However, the expression pattern and roles of microRNA-342-3p in nonsmall cell lung cancer remain poorly understood. In the current study, we explored the roles and underlying mechanisms of microRNA-342-3p in nonsmall cell lung cancer via gain- and loss-of-function analyses. We used quantitative reverse-transcription-polymerase chain reaction and western blotting assays to measure the expression levels of microRNA-342-3p in nonsmall-cell lung cancer and B-cell lymphoma-2. Furthermore, we used small interfering RNA and RNA mimics to analyze the functions and underlying mechanisms of microRNA-342-3p in nonsmall cell lung cancer cells. A luciferase reporter assay was performed to evaluate the direct binding site of the 5′-untranslated region of B-cell lymphoma-2 targeted by microRNA-342-3p. We found that the expression of microRNA-342-3p was significantly lower in nonsmall cell lung cancer cells and tissues than in normal cells and tissues. The upregulation of microRNA-342-3p suppressed cell proliferation while promoting apoptosis in H1975, H460, and H226 cells. The overexpression of microRNA-342-3p in nonsmall cell lung cancer cells led to the downregulation of mRNA and protein levels in B-cell lymphoma-2 cells. Thus, B-cell lymphoma-2 was identified as a direct target of microRNA-342-3p. These findings indicate that microRNA-342-3p inhibits the growth of nonsmall cell lung cancer by repressing the expression of B-cell lymphoma-2, which suggests that microRNA-342-3p could be a potential target for the treatment of nonsmall cell lung cancer. SAGE Publications 2021-09-14 /pmc/articles/PMC8445541/ /pubmed/34520298 http://dx.doi.org/10.1177/15330338211041193 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Article Chen, Zhongjie Ying, Junjie Shang, Wenjun Ding, Dongxiao Guo, Min Wang, Haifeng miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2 |
title | miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2 |
title_full | miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2 |
title_fullStr | miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2 |
title_full_unstemmed | miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2 |
title_short | miR-342-3p Regulates the Proliferation and Apoptosis of NSCLC Cells by Targeting BCL-2 |
title_sort | mir-342-3p regulates the proliferation and apoptosis of nsclc cells by targeting bcl-2 |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445541/ https://www.ncbi.nlm.nih.gov/pubmed/34520298 http://dx.doi.org/10.1177/15330338211041193 |
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