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Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells
Amantadine hydrochloride (HCl) is commonly prescribed for treating influenza A virus infection and Parkinson’s disease. Recently, several studies have indicated that the use of amantadine HCl is associated with corneal edema; however, the cytotoxic effect of amantadine HCl has not been investigated....
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445916/ https://www.ncbi.nlm.nih.gov/pubmed/34531501 http://dx.doi.org/10.1038/s41598-021-98005-9 |
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author | Lee, Po-Yen Lai, Yu-Hung Liu, Po-Len Liu, Ching-Chih Su, Chia-Cheng Chiu, Fang-Yen Cheng, Wei-Chung Hsu, Shiuh-Liang Cheng, Kai-Chun Chiu, Li-Yi Kao, Tzu-En Lin, Chia-Ching Chang, Yo-Chen Wang, Shu-Chi Li, Chia-Yang |
author_facet | Lee, Po-Yen Lai, Yu-Hung Liu, Po-Len Liu, Ching-Chih Su, Chia-Cheng Chiu, Fang-Yen Cheng, Wei-Chung Hsu, Shiuh-Liang Cheng, Kai-Chun Chiu, Li-Yi Kao, Tzu-En Lin, Chia-Ching Chang, Yo-Chen Wang, Shu-Chi Li, Chia-Yang |
author_sort | Lee, Po-Yen |
collection | PubMed |
description | Amantadine hydrochloride (HCl) is commonly prescribed for treating influenza A virus infection and Parkinson’s disease. Recently, several studies have indicated that the use of amantadine HCl is associated with corneal edema; however, the cytotoxic effect of amantadine HCl has not been investigated. In the present study, the effects of amantadine HCl on cell growth, proliferation, and apoptosis in bovine cornea endothelial cells, and in vitro endothelial permeability were examined. Results showed that lower doses of amantadine HCl do not affect cell growth (≤ 20 μΜ), whereas higher doses of amantadine HCl inhibits cell growth (≥ 50 μΜ), induces apoptosis (2000 μΜ), increases sub-G1 phase growth arrest (2000 μΜ), causes DNA damage (≥ 1000 μΜ), and induces endothelial hyperpermeability (≥ 1000 μΜ) in bovine cornea endothelial cells; additionally, we also found that amantadine HCl attenuates the proliferation (≥ 200 μΜ) and arrests cell cycle at G1 phase (≥ 200 μΜ) in bovine cornea endothelial cells. In the present study, we measured the cytotoxic doses of amantadine HCl on cornea endothelial cells, which might be applied in evaluating the association of corneal edema. |
format | Online Article Text |
id | pubmed-8445916 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84459162021-09-20 Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells Lee, Po-Yen Lai, Yu-Hung Liu, Po-Len Liu, Ching-Chih Su, Chia-Cheng Chiu, Fang-Yen Cheng, Wei-Chung Hsu, Shiuh-Liang Cheng, Kai-Chun Chiu, Li-Yi Kao, Tzu-En Lin, Chia-Ching Chang, Yo-Chen Wang, Shu-Chi Li, Chia-Yang Sci Rep Article Amantadine hydrochloride (HCl) is commonly prescribed for treating influenza A virus infection and Parkinson’s disease. Recently, several studies have indicated that the use of amantadine HCl is associated with corneal edema; however, the cytotoxic effect of amantadine HCl has not been investigated. In the present study, the effects of amantadine HCl on cell growth, proliferation, and apoptosis in bovine cornea endothelial cells, and in vitro endothelial permeability were examined. Results showed that lower doses of amantadine HCl do not affect cell growth (≤ 20 μΜ), whereas higher doses of amantadine HCl inhibits cell growth (≥ 50 μΜ), induces apoptosis (2000 μΜ), increases sub-G1 phase growth arrest (2000 μΜ), causes DNA damage (≥ 1000 μΜ), and induces endothelial hyperpermeability (≥ 1000 μΜ) in bovine cornea endothelial cells; additionally, we also found that amantadine HCl attenuates the proliferation (≥ 200 μΜ) and arrests cell cycle at G1 phase (≥ 200 μΜ) in bovine cornea endothelial cells. In the present study, we measured the cytotoxic doses of amantadine HCl on cornea endothelial cells, which might be applied in evaluating the association of corneal edema. Nature Publishing Group UK 2021-09-16 /pmc/articles/PMC8445916/ /pubmed/34531501 http://dx.doi.org/10.1038/s41598-021-98005-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Lee, Po-Yen Lai, Yu-Hung Liu, Po-Len Liu, Ching-Chih Su, Chia-Cheng Chiu, Fang-Yen Cheng, Wei-Chung Hsu, Shiuh-Liang Cheng, Kai-Chun Chiu, Li-Yi Kao, Tzu-En Lin, Chia-Ching Chang, Yo-Chen Wang, Shu-Chi Li, Chia-Yang Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells |
title | Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells |
title_full | Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells |
title_fullStr | Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells |
title_full_unstemmed | Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells |
title_short | Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells |
title_sort | toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445916/ https://www.ncbi.nlm.nih.gov/pubmed/34531501 http://dx.doi.org/10.1038/s41598-021-98005-9 |
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