Highly efficient CRISPR/Cas9 system in Plasmodium falciparum using Cas9-expressing parasites and a linear donor template

The CRISPR/Cas9 system is a powerful genetic engineering technology for Plasmodium falciparum. We here report further improvement of the CRISPR/Cas9 system by combining the Cas9-expressing parasite with a liner donor template DNA. The Cas9-expressing parasite was generated by inserting the cas9 gene...

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Detalles Bibliográficos
Autores principales: Nishi, Tsubasa, Shinzawa, Naoaki, Yuda, Masao, Iwanaga, Shiroh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445982/
https://www.ncbi.nlm.nih.gov/pubmed/34531479
http://dx.doi.org/10.1038/s41598-021-97984-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445982/
https://www.ncbi.nlm.nih.gov/pubmed/34531479
http://dx.doi.org/10.1038/s41598-021-97984-z

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