Cargando…

Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii

In the multidrug resistant (MDR) pathogen Acinetobacter baumannii the global repressor H-NS was shown to modulate the expression of genes involved in pathogenesis and stress response. In addition, H-NS inactivation results in an increased resistance to colistin, and in a hypermotile phenotype an alt...

Descripción completa

Detalles Bibliográficos
Autores principales: Rodgers, Deja, Le, Casin, Pimentel, Camila, Tuttobene, Marisel R., Subils, Tomás, Escalante, Jenny, Nishimura, Brent, Vescovi, Eleonora García, Sieira, Rodrigo, Bonomo, Robert A., Tolmasky, Marcelo E., Ramirez, Maria Soledad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446060/
https://www.ncbi.nlm.nih.gov/pubmed/34531538
http://dx.doi.org/10.1038/s41598-021-98101-w
_version_ 1784568795872886784
author Rodgers, Deja
Le, Casin
Pimentel, Camila
Tuttobene, Marisel R.
Subils, Tomás
Escalante, Jenny
Nishimura, Brent
Vescovi, Eleonora García
Sieira, Rodrigo
Bonomo, Robert A.
Tolmasky, Marcelo E.
Ramirez, Maria Soledad
author_facet Rodgers, Deja
Le, Casin
Pimentel, Camila
Tuttobene, Marisel R.
Subils, Tomás
Escalante, Jenny
Nishimura, Brent
Vescovi, Eleonora García
Sieira, Rodrigo
Bonomo, Robert A.
Tolmasky, Marcelo E.
Ramirez, Maria Soledad
author_sort Rodgers, Deja
collection PubMed
description In the multidrug resistant (MDR) pathogen Acinetobacter baumannii the global repressor H-NS was shown to modulate the expression of genes involved in pathogenesis and stress response. In addition, H-NS inactivation results in an increased resistance to colistin, and in a hypermotile phenotype an altered stress response. To further contribute to the knowledge of this key transcriptional regulator in A. baumannii behavior, we studied the role of H-NS in antimicrobial resistance. Using two well characterized A. baumannii model strains with distinctive resistance profile and pathogenicity traits (AB5075 and A118), complementary transcriptomic and phenotypic approaches were used to study the role of H-NS in antimicrobial resistance, biofilm and quorum sensing gene expression. An increased expression of genes associated with β-lactam resistance, aminoglycosides, quinolones, chloramphenicol, trimethoprim and sulfonamides resistance in the Δhns mutant background was observed. Genes codifying for efflux pumps were also up-regulated, with the exception of adeFGH. The wild-type transcriptional level was restored in the complemented strain. In addition, the expression of biofilm related genes and biofilm production was lowered when the transcriptional repressor was absent. The quorum network genes aidA, abaI, kar and fadD were up-regulated in Δhns mutant strains. Overall, our results showed the complexity and scope of the regulatory network control by H-NS (genes involved in antibiotic resistance and persistence). These observations brings us one step closer to understanding the regulatory role of hns to combat A. baumannii infections.
format Online
Article
Text
id pubmed-8446060
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-84460602021-09-21 Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii Rodgers, Deja Le, Casin Pimentel, Camila Tuttobene, Marisel R. Subils, Tomás Escalante, Jenny Nishimura, Brent Vescovi, Eleonora García Sieira, Rodrigo Bonomo, Robert A. Tolmasky, Marcelo E. Ramirez, Maria Soledad Sci Rep Article In the multidrug resistant (MDR) pathogen Acinetobacter baumannii the global repressor H-NS was shown to modulate the expression of genes involved in pathogenesis and stress response. In addition, H-NS inactivation results in an increased resistance to colistin, and in a hypermotile phenotype an altered stress response. To further contribute to the knowledge of this key transcriptional regulator in A. baumannii behavior, we studied the role of H-NS in antimicrobial resistance. Using two well characterized A. baumannii model strains with distinctive resistance profile and pathogenicity traits (AB5075 and A118), complementary transcriptomic and phenotypic approaches were used to study the role of H-NS in antimicrobial resistance, biofilm and quorum sensing gene expression. An increased expression of genes associated with β-lactam resistance, aminoglycosides, quinolones, chloramphenicol, trimethoprim and sulfonamides resistance in the Δhns mutant background was observed. Genes codifying for efflux pumps were also up-regulated, with the exception of adeFGH. The wild-type transcriptional level was restored in the complemented strain. In addition, the expression of biofilm related genes and biofilm production was lowered when the transcriptional repressor was absent. The quorum network genes aidA, abaI, kar and fadD were up-regulated in Δhns mutant strains. Overall, our results showed the complexity and scope of the regulatory network control by H-NS (genes involved in antibiotic resistance and persistence). These observations brings us one step closer to understanding the regulatory role of hns to combat A. baumannii infections. Nature Publishing Group UK 2021-09-16 /pmc/articles/PMC8446060/ /pubmed/34531538 http://dx.doi.org/10.1038/s41598-021-98101-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Rodgers, Deja
Le, Casin
Pimentel, Camila
Tuttobene, Marisel R.
Subils, Tomás
Escalante, Jenny
Nishimura, Brent
Vescovi, Eleonora García
Sieira, Rodrigo
Bonomo, Robert A.
Tolmasky, Marcelo E.
Ramirez, Maria Soledad
Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii
title Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii
title_full Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii
title_fullStr Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii
title_full_unstemmed Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii
title_short Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii
title_sort histone-like nucleoid-structuring protein (h-ns) regulatory role in antibiotic resistance in acinetobacter baumannii
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446060/
https://www.ncbi.nlm.nih.gov/pubmed/34531538
http://dx.doi.org/10.1038/s41598-021-98101-w
work_keys_str_mv AT rodgersdeja histonelikenucleoidstructuringproteinhnsregulatoryroleinantibioticresistanceinacinetobacterbaumannii
AT lecasin histonelikenucleoidstructuringproteinhnsregulatoryroleinantibioticresistanceinacinetobacterbaumannii
AT pimentelcamila histonelikenucleoidstructuringproteinhnsregulatoryroleinantibioticresistanceinacinetobacterbaumannii
AT tuttobenemariselr histonelikenucleoidstructuringproteinhnsregulatoryroleinantibioticresistanceinacinetobacterbaumannii
AT subilstomas histonelikenucleoidstructuringproteinhnsregulatoryroleinantibioticresistanceinacinetobacterbaumannii
AT escalantejenny histonelikenucleoidstructuringproteinhnsregulatoryroleinantibioticresistanceinacinetobacterbaumannii
AT nishimurabrent histonelikenucleoidstructuringproteinhnsregulatoryroleinantibioticresistanceinacinetobacterbaumannii
AT vescovieleonoragarcia histonelikenucleoidstructuringproteinhnsregulatoryroleinantibioticresistanceinacinetobacterbaumannii
AT sieirarodrigo histonelikenucleoidstructuringproteinhnsregulatoryroleinantibioticresistanceinacinetobacterbaumannii
AT bonomoroberta histonelikenucleoidstructuringproteinhnsregulatoryroleinantibioticresistanceinacinetobacterbaumannii
AT tolmaskymarceloe histonelikenucleoidstructuringproteinhnsregulatoryroleinantibioticresistanceinacinetobacterbaumannii
AT ramirezmariasoledad histonelikenucleoidstructuringproteinhnsregulatoryroleinantibioticresistanceinacinetobacterbaumannii