Cargando…
A novel ATPase gene, Ab-atps, plays an important role in the interaction of rice and white tip nematode, Aphelenchoides besseyi
Plant kinases containing the LysM domain play important roles in pathogen recognition and self-defense reactions. And it could recognize microbe-associated molecules including chitin and other polypeptides. The white tip nematode Aphelenchoides besseyi is a migratory parasitic nematode that infects...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446066/ https://www.ncbi.nlm.nih.gov/pubmed/34531469 http://dx.doi.org/10.1038/s41598-021-97981-2 |
_version_ | 1784568797398564864 |
---|---|
author | Wang, Hong-Le Xu, Chun-Ling Chen, Chun Ding, Shan-Wen Li, Jun-Yi Yang, Si-Hua Xie, Hui |
author_facet | Wang, Hong-Le Xu, Chun-Ling Chen, Chun Ding, Shan-Wen Li, Jun-Yi Yang, Si-Hua Xie, Hui |
author_sort | Wang, Hong-Le |
collection | PubMed |
description | Plant kinases containing the LysM domain play important roles in pathogen recognition and self-defense reactions. And it could recognize microbe-associated molecules including chitin and other polypeptides. The white tip nematode Aphelenchoides besseyi is a migratory parasitic nematode that infects plant shoots. It is distributed over almost all rice-producing areas and causes up to 50% economic losses. The rice OsRLK3 gene was a defense-related LysM kinase gene of rice. This study showed that the rice LysM kinase OsRLK3 could be induced by flg22, jasmonic acid, salicylic acid, and chitin. An interaction gene, Ab-atps from A. besseyi, was identified by screening the interaction between the rice gene OsRLK3 and an A. besseyi cDNA library using yeast two-hybrid screening. Ab-atps is a novel ATP synthase gene with a full length of 1341 bp, coding for 183 amino acids. The mRNA of Ab-atps was located in the esophagus and reproductive system of A. besseyi. The expression of Ab-atps was assessed at different developmental stages of the nematode and found to be the highest in the juvenile, followed by the egg, female, and male. Reproduction was significantly decreased in nematodes treated with Ab-atps double-stranded RNA (dsRNA) (p < 0.05). Transient expression experiments showed that Ab-ATPS-GFP was distributed in the nucleus, cytoplasm, and cell membrane, and Ab-ATPS-GFP triggered plant cell death. OsRLK3 was expressed significantly higher at 0.5 day and 1 day (p < 0.05) in rice plants inoculated with nematodes treated with Ab-atps dsRNA and gfp dsRNA for 0.5–7 days, respectively. Further, OsRLK3 expression under Ab-atps dsRNA treatment was significantly lower than with gfp dsRNA treatment at 0.5 day (p < 0.05) and significantly higher than with gfp dsRNA treatment at 1 day (p < 0.05). These results suggest that rice OsRLK3 could interact with A. besseyi Ab-atps, which plays an important role in growth, reproduction, and infection of the nematode. Our findings provide a theoretical basis to further understand the parasitic strategy of A. besseyi and its interaction mechanism with host plants, suggesting new ideas and targets for controlling A. besseyi. |
format | Online Article Text |
id | pubmed-8446066 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-84460662021-09-21 A novel ATPase gene, Ab-atps, plays an important role in the interaction of rice and white tip nematode, Aphelenchoides besseyi Wang, Hong-Le Xu, Chun-Ling Chen, Chun Ding, Shan-Wen Li, Jun-Yi Yang, Si-Hua Xie, Hui Sci Rep Article Plant kinases containing the LysM domain play important roles in pathogen recognition and self-defense reactions. And it could recognize microbe-associated molecules including chitin and other polypeptides. The white tip nematode Aphelenchoides besseyi is a migratory parasitic nematode that infects plant shoots. It is distributed over almost all rice-producing areas and causes up to 50% economic losses. The rice OsRLK3 gene was a defense-related LysM kinase gene of rice. This study showed that the rice LysM kinase OsRLK3 could be induced by flg22, jasmonic acid, salicylic acid, and chitin. An interaction gene, Ab-atps from A. besseyi, was identified by screening the interaction between the rice gene OsRLK3 and an A. besseyi cDNA library using yeast two-hybrid screening. Ab-atps is a novel ATP synthase gene with a full length of 1341 bp, coding for 183 amino acids. The mRNA of Ab-atps was located in the esophagus and reproductive system of A. besseyi. The expression of Ab-atps was assessed at different developmental stages of the nematode and found to be the highest in the juvenile, followed by the egg, female, and male. Reproduction was significantly decreased in nematodes treated with Ab-atps double-stranded RNA (dsRNA) (p < 0.05). Transient expression experiments showed that Ab-ATPS-GFP was distributed in the nucleus, cytoplasm, and cell membrane, and Ab-ATPS-GFP triggered plant cell death. OsRLK3 was expressed significantly higher at 0.5 day and 1 day (p < 0.05) in rice plants inoculated with nematodes treated with Ab-atps dsRNA and gfp dsRNA for 0.5–7 days, respectively. Further, OsRLK3 expression under Ab-atps dsRNA treatment was significantly lower than with gfp dsRNA treatment at 0.5 day (p < 0.05) and significantly higher than with gfp dsRNA treatment at 1 day (p < 0.05). These results suggest that rice OsRLK3 could interact with A. besseyi Ab-atps, which plays an important role in growth, reproduction, and infection of the nematode. Our findings provide a theoretical basis to further understand the parasitic strategy of A. besseyi and its interaction mechanism with host plants, suggesting new ideas and targets for controlling A. besseyi. Nature Publishing Group UK 2021-09-16 /pmc/articles/PMC8446066/ /pubmed/34531469 http://dx.doi.org/10.1038/s41598-021-97981-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Wang, Hong-Le Xu, Chun-Ling Chen, Chun Ding, Shan-Wen Li, Jun-Yi Yang, Si-Hua Xie, Hui A novel ATPase gene, Ab-atps, plays an important role in the interaction of rice and white tip nematode, Aphelenchoides besseyi |
title | A novel ATPase gene, Ab-atps, plays an important role in the interaction of rice and white tip nematode, Aphelenchoides besseyi |
title_full | A novel ATPase gene, Ab-atps, plays an important role in the interaction of rice and white tip nematode, Aphelenchoides besseyi |
title_fullStr | A novel ATPase gene, Ab-atps, plays an important role in the interaction of rice and white tip nematode, Aphelenchoides besseyi |
title_full_unstemmed | A novel ATPase gene, Ab-atps, plays an important role in the interaction of rice and white tip nematode, Aphelenchoides besseyi |
title_short | A novel ATPase gene, Ab-atps, plays an important role in the interaction of rice and white tip nematode, Aphelenchoides besseyi |
title_sort | novel atpase gene, ab-atps, plays an important role in the interaction of rice and white tip nematode, aphelenchoides besseyi |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446066/ https://www.ncbi.nlm.nih.gov/pubmed/34531469 http://dx.doi.org/10.1038/s41598-021-97981-2 |
work_keys_str_mv | AT wanghongle anovelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi AT xuchunling anovelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi AT chenchun anovelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi AT dingshanwen anovelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi AT lijunyi anovelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi AT yangsihua anovelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi AT xiehui anovelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi AT wanghongle novelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi AT xuchunling novelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi AT chenchun novelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi AT dingshanwen novelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi AT lijunyi novelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi AT yangsihua novelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi AT xiehui novelatpasegeneabatpsplaysanimportantroleintheinteractionofriceandwhitetipnematodeaphelenchoidesbesseyi |