Cargando…
Transplantation of a vascularized pedicle of hemisected spinal cord to establish spinal cord continuity after removal of a segment of the thoracic spinal cord: A proof‐of‐principle study in dogs
INTRODUCTION: Glial scar formation impedes nerve regeneration/reinnervation after spinal cord injury (SCI); therefore, removal of scar tissue is essential for SCI treatment. AIMS: To investigate whether removing a spinal cord and transplanting a vascularized pedicle of hemisected spinal cord from th...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446222/ https://www.ncbi.nlm.nih.gov/pubmed/34184402 http://dx.doi.org/10.1111/cns.13696 |
_version_ | 1784568827565047808 |
---|---|
author | Ren, Shuai Zhang, Weihua Liu, HongMiao Wang, Xin Guan, Xiangchen Zhang, Mingzhe Zhang, Jian Wu, Qiong Xue, Yan Wang, Dan Liu, Yong Liu, Jianyu Ren, Xiaoping |
author_facet | Ren, Shuai Zhang, Weihua Liu, HongMiao Wang, Xin Guan, Xiangchen Zhang, Mingzhe Zhang, Jian Wu, Qiong Xue, Yan Wang, Dan Liu, Yong Liu, Jianyu Ren, Xiaoping |
author_sort | Ren, Shuai |
collection | PubMed |
description | INTRODUCTION: Glial scar formation impedes nerve regeneration/reinnervation after spinal cord injury (SCI); therefore, removal of scar tissue is essential for SCI treatment. AIMS: To investigate whether removing a spinal cord and transplanting a vascularized pedicle of hemisected spinal cord from the spinal cord caudal to the transection can restore motor function, to aid in the treatment of future clinical spinal cord injuries. We developed a canine model. After removal of a 1‐cm segment of the thoracic (T10–T11) spinal cord in eight beagles, a vascularized pedicle of hemisected spinal cord from the first 1.5 cm of the spinal cord caudal to the transection (cut along the posterior median sulcus of the spinal cord) was transplanted to bridge the transected spinal cord in the presence of a fusogen (polyethylene glycol, PEG) in four of the eight dogs. We used various forms of imaging, electron microscopy, and histologic data to determine that after our transplantation of a vascular pedicled hemisection to bridge the transected spinal cord, electrical continuity across the spinal bridge was restored. RESULTS: Motor function was restored following our transplantation, as confirmed by the re‐establishment of anatomic continuity along with interfacial axonal sprouting. CONCLUSION: Taken together, our findings suggest that SCI patients—who have previously been thought to have irreversible damage and/or paralysis—may be treated effectively with similar operative techniques to re‐establish electrical and functional continuity following SCI. |
format | Online Article Text |
id | pubmed-8446222 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84462222021-09-22 Transplantation of a vascularized pedicle of hemisected spinal cord to establish spinal cord continuity after removal of a segment of the thoracic spinal cord: A proof‐of‐principle study in dogs Ren, Shuai Zhang, Weihua Liu, HongMiao Wang, Xin Guan, Xiangchen Zhang, Mingzhe Zhang, Jian Wu, Qiong Xue, Yan Wang, Dan Liu, Yong Liu, Jianyu Ren, Xiaoping CNS Neurosci Ther Original Articles INTRODUCTION: Glial scar formation impedes nerve regeneration/reinnervation after spinal cord injury (SCI); therefore, removal of scar tissue is essential for SCI treatment. AIMS: To investigate whether removing a spinal cord and transplanting a vascularized pedicle of hemisected spinal cord from the spinal cord caudal to the transection can restore motor function, to aid in the treatment of future clinical spinal cord injuries. We developed a canine model. After removal of a 1‐cm segment of the thoracic (T10–T11) spinal cord in eight beagles, a vascularized pedicle of hemisected spinal cord from the first 1.5 cm of the spinal cord caudal to the transection (cut along the posterior median sulcus of the spinal cord) was transplanted to bridge the transected spinal cord in the presence of a fusogen (polyethylene glycol, PEG) in four of the eight dogs. We used various forms of imaging, electron microscopy, and histologic data to determine that after our transplantation of a vascular pedicled hemisection to bridge the transected spinal cord, electrical continuity across the spinal bridge was restored. RESULTS: Motor function was restored following our transplantation, as confirmed by the re‐establishment of anatomic continuity along with interfacial axonal sprouting. CONCLUSION: Taken together, our findings suggest that SCI patients—who have previously been thought to have irreversible damage and/or paralysis—may be treated effectively with similar operative techniques to re‐establish electrical and functional continuity following SCI. John Wiley and Sons Inc. 2021-06-28 /pmc/articles/PMC8446222/ /pubmed/34184402 http://dx.doi.org/10.1111/cns.13696 Text en © 2021 The Authors. CNS Neuroscience & Therapeutics published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Ren, Shuai Zhang, Weihua Liu, HongMiao Wang, Xin Guan, Xiangchen Zhang, Mingzhe Zhang, Jian Wu, Qiong Xue, Yan Wang, Dan Liu, Yong Liu, Jianyu Ren, Xiaoping Transplantation of a vascularized pedicle of hemisected spinal cord to establish spinal cord continuity after removal of a segment of the thoracic spinal cord: A proof‐of‐principle study in dogs |
title | Transplantation of a vascularized pedicle of hemisected spinal cord to establish spinal cord continuity after removal of a segment of the thoracic spinal cord: A proof‐of‐principle study in dogs |
title_full | Transplantation of a vascularized pedicle of hemisected spinal cord to establish spinal cord continuity after removal of a segment of the thoracic spinal cord: A proof‐of‐principle study in dogs |
title_fullStr | Transplantation of a vascularized pedicle of hemisected spinal cord to establish spinal cord continuity after removal of a segment of the thoracic spinal cord: A proof‐of‐principle study in dogs |
title_full_unstemmed | Transplantation of a vascularized pedicle of hemisected spinal cord to establish spinal cord continuity after removal of a segment of the thoracic spinal cord: A proof‐of‐principle study in dogs |
title_short | Transplantation of a vascularized pedicle of hemisected spinal cord to establish spinal cord continuity after removal of a segment of the thoracic spinal cord: A proof‐of‐principle study in dogs |
title_sort | transplantation of a vascularized pedicle of hemisected spinal cord to establish spinal cord continuity after removal of a segment of the thoracic spinal cord: a proof‐of‐principle study in dogs |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446222/ https://www.ncbi.nlm.nih.gov/pubmed/34184402 http://dx.doi.org/10.1111/cns.13696 |
work_keys_str_mv | AT renshuai transplantationofavascularizedpedicleofhemisectedspinalcordtoestablishspinalcordcontinuityafterremovalofasegmentofthethoracicspinalcordaproofofprinciplestudyindogs AT zhangweihua transplantationofavascularizedpedicleofhemisectedspinalcordtoestablishspinalcordcontinuityafterremovalofasegmentofthethoracicspinalcordaproofofprinciplestudyindogs AT liuhongmiao transplantationofavascularizedpedicleofhemisectedspinalcordtoestablishspinalcordcontinuityafterremovalofasegmentofthethoracicspinalcordaproofofprinciplestudyindogs AT wangxin transplantationofavascularizedpedicleofhemisectedspinalcordtoestablishspinalcordcontinuityafterremovalofasegmentofthethoracicspinalcordaproofofprinciplestudyindogs AT guanxiangchen transplantationofavascularizedpedicleofhemisectedspinalcordtoestablishspinalcordcontinuityafterremovalofasegmentofthethoracicspinalcordaproofofprinciplestudyindogs AT zhangmingzhe transplantationofavascularizedpedicleofhemisectedspinalcordtoestablishspinalcordcontinuityafterremovalofasegmentofthethoracicspinalcordaproofofprinciplestudyindogs AT zhangjian transplantationofavascularizedpedicleofhemisectedspinalcordtoestablishspinalcordcontinuityafterremovalofasegmentofthethoracicspinalcordaproofofprinciplestudyindogs AT wuqiong transplantationofavascularizedpedicleofhemisectedspinalcordtoestablishspinalcordcontinuityafterremovalofasegmentofthethoracicspinalcordaproofofprinciplestudyindogs AT xueyan transplantationofavascularizedpedicleofhemisectedspinalcordtoestablishspinalcordcontinuityafterremovalofasegmentofthethoracicspinalcordaproofofprinciplestudyindogs AT wangdan transplantationofavascularizedpedicleofhemisectedspinalcordtoestablishspinalcordcontinuityafterremovalofasegmentofthethoracicspinalcordaproofofprinciplestudyindogs AT liuyong transplantationofavascularizedpedicleofhemisectedspinalcordtoestablishspinalcordcontinuityafterremovalofasegmentofthethoracicspinalcordaproofofprinciplestudyindogs AT liujianyu transplantationofavascularizedpedicleofhemisectedspinalcordtoestablishspinalcordcontinuityafterremovalofasegmentofthethoracicspinalcordaproofofprinciplestudyindogs AT renxiaoping transplantationofavascularizedpedicleofhemisectedspinalcordtoestablishspinalcordcontinuityafterremovalofasegmentofthethoracicspinalcordaproofofprinciplestudyindogs |