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Identification of Immune Activation Markers in the Early Onset of COVID-19 Infection

This study aimed to determine the specific cytokine profile in peripheral blood during the early onset of COVID-19 infection. This was a cross-sectional exploratory, single center study. A total of 55 plasma samples were studied. Serum samples of adults showing symptoms of COVID-19 infection who wer...

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Autores principales: Kovarik, Johannes J., Kämpf, Anna K., Gasser, Fabian, Herdina, Anna N., Breuer, Monika, Kaltenecker, Christopher C., Wahrmann, Markus, Haindl, Susanne, Mayer, Florian, Traby, Ludwig, Touzeau-Roemer, Veronique, Grabmeier-Pfistershammer, Katharina, Kussmann, Manuel, Robak, Oliver, Willschke, Harald, Ay, Care, Säemann, Marcus D., Schmetterer, Klaus G., Strassl, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446609/
https://www.ncbi.nlm.nih.gov/pubmed/34540715
http://dx.doi.org/10.3389/fcimb.2021.651484
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author Kovarik, Johannes J.
Kämpf, Anna K.
Gasser, Fabian
Herdina, Anna N.
Breuer, Monika
Kaltenecker, Christopher C.
Wahrmann, Markus
Haindl, Susanne
Mayer, Florian
Traby, Ludwig
Touzeau-Roemer, Veronique
Grabmeier-Pfistershammer, Katharina
Kussmann, Manuel
Robak, Oliver
Willschke, Harald
Ay, Care
Säemann, Marcus D.
Schmetterer, Klaus G.
Strassl, Robert
author_facet Kovarik, Johannes J.
Kämpf, Anna K.
Gasser, Fabian
Herdina, Anna N.
Breuer, Monika
Kaltenecker, Christopher C.
Wahrmann, Markus
Haindl, Susanne
Mayer, Florian
Traby, Ludwig
Touzeau-Roemer, Veronique
Grabmeier-Pfistershammer, Katharina
Kussmann, Manuel
Robak, Oliver
Willschke, Harald
Ay, Care
Säemann, Marcus D.
Schmetterer, Klaus G.
Strassl, Robert
author_sort Kovarik, Johannes J.
collection PubMed
description This study aimed to determine the specific cytokine profile in peripheral blood during the early onset of COVID-19 infection. This was a cross-sectional exploratory, single center study. A total of 55 plasma samples were studied. Serum samples of adults showing symptoms of COVID-19 infection who were tested positive for SARS-CoV-2 infection (CoV+, n=18) at the COVID-19 outpatient clinic of the Medical University of Vienna were screened for immune activation markers by Luminex technology. Additionally, age and gender-matched serum samples of patients displaying COVID-19 associated symptoms, but tested negative for SARS-CoV-2 (CoV-, n=16) as well as healthy controls (HC, n=21) were analyzed. COVID-19 positive (CoV+) patients showed a specific upregulation of BLC (141; 74-189 pg/mL), SCD30 (273; 207-576 pg/mL), MCP-2 (18; 12-30 pg/mL) and IP-10 (37; 23-96 pg/mL), compared to patients with COVID19-like symptoms but negative PCR test (CoV-), BLC (61; 22-100 pg/mL), sCD30L (161; 120-210 pg/mL), MCP-2 (8; 5-12 pg/mL) and IP-10 (9; 6-12 pg/mL) and healthy controls (HC) (BLC 22; 11-36 pg/mL, sCD30 74; 39-108 pg/mL, MCP-2 6; 3-9. pg/mL, IP-10 = 8; 5-13). The markers APRIL, sIL-2R, IL7, MIF, MIP-1b, SCF, SDF-1a, sTNF-RII were elevated in both CoV+ and CoV- patient groups compared to healthy controls. HGF, MDC and VEGF-A were elevated in CoV- but not CoV+ compared to healthy controls. BLC, sCD30, MCP-2 and IP-10 are specifically induced during early stages of COVID-19 infection and might constitute attractive targets for early diagnosis and treatment of this disease.
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spelling pubmed-84466092021-09-18 Identification of Immune Activation Markers in the Early Onset of COVID-19 Infection Kovarik, Johannes J. Kämpf, Anna K. Gasser, Fabian Herdina, Anna N. Breuer, Monika Kaltenecker, Christopher C. Wahrmann, Markus Haindl, Susanne Mayer, Florian Traby, Ludwig Touzeau-Roemer, Veronique Grabmeier-Pfistershammer, Katharina Kussmann, Manuel Robak, Oliver Willschke, Harald Ay, Care Säemann, Marcus D. Schmetterer, Klaus G. Strassl, Robert Front Cell Infect Microbiol Cellular and Infection Microbiology This study aimed to determine the specific cytokine profile in peripheral blood during the early onset of COVID-19 infection. This was a cross-sectional exploratory, single center study. A total of 55 plasma samples were studied. Serum samples of adults showing symptoms of COVID-19 infection who were tested positive for SARS-CoV-2 infection (CoV+, n=18) at the COVID-19 outpatient clinic of the Medical University of Vienna were screened for immune activation markers by Luminex technology. Additionally, age and gender-matched serum samples of patients displaying COVID-19 associated symptoms, but tested negative for SARS-CoV-2 (CoV-, n=16) as well as healthy controls (HC, n=21) were analyzed. COVID-19 positive (CoV+) patients showed a specific upregulation of BLC (141; 74-189 pg/mL), SCD30 (273; 207-576 pg/mL), MCP-2 (18; 12-30 pg/mL) and IP-10 (37; 23-96 pg/mL), compared to patients with COVID19-like symptoms but negative PCR test (CoV-), BLC (61; 22-100 pg/mL), sCD30L (161; 120-210 pg/mL), MCP-2 (8; 5-12 pg/mL) and IP-10 (9; 6-12 pg/mL) and healthy controls (HC) (BLC 22; 11-36 pg/mL, sCD30 74; 39-108 pg/mL, MCP-2 6; 3-9. pg/mL, IP-10 = 8; 5-13). The markers APRIL, sIL-2R, IL7, MIF, MIP-1b, SCF, SDF-1a, sTNF-RII were elevated in both CoV+ and CoV- patient groups compared to healthy controls. HGF, MDC and VEGF-A were elevated in CoV- but not CoV+ compared to healthy controls. BLC, sCD30, MCP-2 and IP-10 are specifically induced during early stages of COVID-19 infection and might constitute attractive targets for early diagnosis and treatment of this disease. Frontiers Media S.A. 2021-09-03 /pmc/articles/PMC8446609/ /pubmed/34540715 http://dx.doi.org/10.3389/fcimb.2021.651484 Text en Copyright © 2021 Kovarik, Kämpf, Gasser, Herdina, Breuer, Kaltenecker, Wahrmann, Haindl, Mayer, Traby, Touzeau-Roemer, Grabmeier-Pfistershammer, Kussmann, Robak, Willschke, Ay, Säemann, Schmetterer and Strassl https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Kovarik, Johannes J.
Kämpf, Anna K.
Gasser, Fabian
Herdina, Anna N.
Breuer, Monika
Kaltenecker, Christopher C.
Wahrmann, Markus
Haindl, Susanne
Mayer, Florian
Traby, Ludwig
Touzeau-Roemer, Veronique
Grabmeier-Pfistershammer, Katharina
Kussmann, Manuel
Robak, Oliver
Willschke, Harald
Ay, Care
Säemann, Marcus D.
Schmetterer, Klaus G.
Strassl, Robert
Identification of Immune Activation Markers in the Early Onset of COVID-19 Infection
title Identification of Immune Activation Markers in the Early Onset of COVID-19 Infection
title_full Identification of Immune Activation Markers in the Early Onset of COVID-19 Infection
title_fullStr Identification of Immune Activation Markers in the Early Onset of COVID-19 Infection
title_full_unstemmed Identification of Immune Activation Markers in the Early Onset of COVID-19 Infection
title_short Identification of Immune Activation Markers in the Early Onset of COVID-19 Infection
title_sort identification of immune activation markers in the early onset of covid-19 infection
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446609/
https://www.ncbi.nlm.nih.gov/pubmed/34540715
http://dx.doi.org/10.3389/fcimb.2021.651484
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