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Nitric Oxide Detoxification by Mesorhizobium loti Affects Root Nodule Symbiosis with Lotus japonicus

Root nodule symbiosis between legumes and rhizobia involves nitric oxide (NO) regulation by both the host plant and symbiotic rhizobia. However, the mechanisms by which the rhizobial control of NO affects root nodule symbiosis in Lotus japonicus are unknown. Therefore, we herein investigated the eff...

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Autores principales: Fukudome, Mitsutaka, Shimokawa, Yuta, Hashimoto, Shun, Maesako, Yusuke, Uchi-Fukudome, Nahoko, Niihara, Kota, Osuki, Ken-ichi, Uchiumi, Toshiki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446750/
https://www.ncbi.nlm.nih.gov/pubmed/34470944
http://dx.doi.org/10.1264/jsme2.ME21038
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author Fukudome, Mitsutaka
Shimokawa, Yuta
Hashimoto, Shun
Maesako, Yusuke
Uchi-Fukudome, Nahoko
Niihara, Kota
Osuki, Ken-ichi
Uchiumi, Toshiki
author_facet Fukudome, Mitsutaka
Shimokawa, Yuta
Hashimoto, Shun
Maesako, Yusuke
Uchi-Fukudome, Nahoko
Niihara, Kota
Osuki, Ken-ichi
Uchiumi, Toshiki
author_sort Fukudome, Mitsutaka
collection PubMed
description Root nodule symbiosis between legumes and rhizobia involves nitric oxide (NO) regulation by both the host plant and symbiotic rhizobia. However, the mechanisms by which the rhizobial control of NO affects root nodule symbiosis in Lotus japonicus are unknown. Therefore, we herein investigated the effects of enhanced NO removal by Mesorhizobium loti on symbiosis with L. japonicus. The hmp gene, which in Sinorhizobium meliloti encodes a flavohemoglobin involved in NO detoxification, was introduced into M. loti to generate a transconjugant with enhanced NO removal. The symbiotic phenotype of the transconjugant with L. japonicus was examined. The transconjugant showed delayed infection and higher nitrogenase activity in mature nodules than the wild type, whereas nodule senescence was normal. This result is in contrast to previous findings showing that enhanced NO removal in L. japonicus by class 1 phytoglobin affected nodule senescence. To evaluate differences in NO detoxification between M. loti and L. japonicus, NO localization in nodules was investigated. The enhanced expression of class 1 phytoglobin in L. japonicus reduced the amount of NO not only in infected cells, but also in vascular bundles, whereas that of hmp in M. loti reduced the amount of NO in infected cells only. This difference suggests that NO detoxification by M. loti exerts different effects in symbiosis than that by L. japonicus.
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spelling pubmed-84467502021-09-29 Nitric Oxide Detoxification by Mesorhizobium loti Affects Root Nodule Symbiosis with Lotus japonicus Fukudome, Mitsutaka Shimokawa, Yuta Hashimoto, Shun Maesako, Yusuke Uchi-Fukudome, Nahoko Niihara, Kota Osuki, Ken-ichi Uchiumi, Toshiki Microbes Environ Regular Paper Root nodule symbiosis between legumes and rhizobia involves nitric oxide (NO) regulation by both the host plant and symbiotic rhizobia. However, the mechanisms by which the rhizobial control of NO affects root nodule symbiosis in Lotus japonicus are unknown. Therefore, we herein investigated the effects of enhanced NO removal by Mesorhizobium loti on symbiosis with L. japonicus. The hmp gene, which in Sinorhizobium meliloti encodes a flavohemoglobin involved in NO detoxification, was introduced into M. loti to generate a transconjugant with enhanced NO removal. The symbiotic phenotype of the transconjugant with L. japonicus was examined. The transconjugant showed delayed infection and higher nitrogenase activity in mature nodules than the wild type, whereas nodule senescence was normal. This result is in contrast to previous findings showing that enhanced NO removal in L. japonicus by class 1 phytoglobin affected nodule senescence. To evaluate differences in NO detoxification between M. loti and L. japonicus, NO localization in nodules was investigated. The enhanced expression of class 1 phytoglobin in L. japonicus reduced the amount of NO not only in infected cells, but also in vascular bundles, whereas that of hmp in M. loti reduced the amount of NO in infected cells only. This difference suggests that NO detoxification by M. loti exerts different effects in symbiosis than that by L. japonicus. Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2021 2021-08-31 /pmc/articles/PMC8446750/ /pubmed/34470944 http://dx.doi.org/10.1264/jsme2.ME21038 Text en 2021 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Paper
Fukudome, Mitsutaka
Shimokawa, Yuta
Hashimoto, Shun
Maesako, Yusuke
Uchi-Fukudome, Nahoko
Niihara, Kota
Osuki, Ken-ichi
Uchiumi, Toshiki
Nitric Oxide Detoxification by Mesorhizobium loti Affects Root Nodule Symbiosis with Lotus japonicus
title Nitric Oxide Detoxification by Mesorhizobium loti Affects Root Nodule Symbiosis with Lotus japonicus
title_full Nitric Oxide Detoxification by Mesorhizobium loti Affects Root Nodule Symbiosis with Lotus japonicus
title_fullStr Nitric Oxide Detoxification by Mesorhizobium loti Affects Root Nodule Symbiosis with Lotus japonicus
title_full_unstemmed Nitric Oxide Detoxification by Mesorhizobium loti Affects Root Nodule Symbiosis with Lotus japonicus
title_short Nitric Oxide Detoxification by Mesorhizobium loti Affects Root Nodule Symbiosis with Lotus japonicus
title_sort nitric oxide detoxification by mesorhizobium loti affects root nodule symbiosis with lotus japonicus
topic Regular Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446750/
https://www.ncbi.nlm.nih.gov/pubmed/34470944
http://dx.doi.org/10.1264/jsme2.ME21038
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