A deletion in the N gene may cause diagnostic escape in SARS-CoV-2 samples

Five SARS-CoV-2-positive samples showed N-gene drop-out with a RT-PCR multiplex test. WGS found all samples to harbor a deletion in the same region of the N gene, which is likely to impair the efficiency of amplification. This highlights the need for a continued surveillance of viral evolution and d...

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Autores principales: Zannoli, Silvia, Dirani, Giorgio, Taddei, Francesca, Gatti, Giulia, Poggianti, Ilaria, Denicolò, Agnese, Arfilli, Valentina, Manera, Martina, Mancini, Andrea, Battisti, Arianna, Sambri, Vittorio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 2022
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447549/
https://www.ncbi.nlm.nih.gov/pubmed/34649189
http://dx.doi.org/10.1016/j.diagmicrobio.2021.115540
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author Zannoli, Silvia
Dirani, Giorgio
Taddei, Francesca
Gatti, Giulia
Poggianti, Ilaria
Denicolò, Agnese
Arfilli, Valentina
Manera, Martina
Mancini, Andrea
Battisti, Arianna
Sambri, Vittorio
author_facet Zannoli, Silvia
Dirani, Giorgio
Taddei, Francesca
Gatti, Giulia
Poggianti, Ilaria
Denicolò, Agnese
Arfilli, Valentina
Manera, Martina
Mancini, Andrea
Battisti, Arianna
Sambri, Vittorio
author_sort Zannoli, Silvia
collection PubMed
description Five SARS-CoV-2-positive samples showed N-gene drop-out with a RT-PCR multiplex test. WGS found all samples to harbor a deletion in the same region of the N gene, which is likely to impair the efficiency of amplification. This highlights the need for a continued surveillance of viral evolution and diagnostic test performance.
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spelling pubmed-84475492021-09-17 A deletion in the N gene may cause diagnostic escape in SARS-CoV-2 samples Zannoli, Silvia Dirani, Giorgio Taddei, Francesca Gatti, Giulia Poggianti, Ilaria Denicolò, Agnese Arfilli, Valentina Manera, Martina Mancini, Andrea Battisti, Arianna Sambri, Vittorio Diagn Microbiol Infect Dis Article Five SARS-CoV-2-positive samples showed N-gene drop-out with a RT-PCR multiplex test. WGS found all samples to harbor a deletion in the same region of the N gene, which is likely to impair the efficiency of amplification. This highlights the need for a continued surveillance of viral evolution and diagnostic test performance. Published by Elsevier Inc. 2022-01 2021-09-17 /pmc/articles/PMC8447549/ /pubmed/34649189 http://dx.doi.org/10.1016/j.diagmicrobio.2021.115540 Text en © 2021 Published by Elsevier Inc. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Zannoli, Silvia
Dirani, Giorgio
Taddei, Francesca
Gatti, Giulia
Poggianti, Ilaria
Denicolò, Agnese
Arfilli, Valentina
Manera, Martina
Mancini, Andrea
Battisti, Arianna
Sambri, Vittorio
A deletion in the N gene may cause diagnostic escape in SARS-CoV-2 samples
title A deletion in the N gene may cause diagnostic escape in SARS-CoV-2 samples
title_full A deletion in the N gene may cause diagnostic escape in SARS-CoV-2 samples
title_fullStr A deletion in the N gene may cause diagnostic escape in SARS-CoV-2 samples
title_full_unstemmed A deletion in the N gene may cause diagnostic escape in SARS-CoV-2 samples
title_short A deletion in the N gene may cause diagnostic escape in SARS-CoV-2 samples
title_sort deletion in the n gene may cause diagnostic escape in sars-cov-2 samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447549/
https://www.ncbi.nlm.nih.gov/pubmed/34649189
http://dx.doi.org/10.1016/j.diagmicrobio.2021.115540
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