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Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation

Rhipicephalus (Boophilus) microplus ticks are obligatory hematophagous ectoparasites of cattle and act as vectors for disease-causing microorganisms. Conventional tick control is based on the application of chemical acaricides; however, their uncontrolled use has increased resistant tick populations...

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Autores principales: Martínez Velázquez, Moisés, Barragán Álvarez, Carla Patricia, Flores Fernández, José Miguel, Lagunes Quintanilla, Rodolfo Esteban, Saines, Edgar Castro, Ramírez Rodríguez, Patricia Berenice, Herrera Rodríguez, Sara Elisa, Gutiérrez, Rodolfo Hernández, Ortega, Abel Gutiérrez, Álvarez, Ángel H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: EDP Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447868/
https://www.ncbi.nlm.nih.gov/pubmed/34533454
http://dx.doi.org/10.1051/parasite/2021064
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author Martínez Velázquez, Moisés
Barragán Álvarez, Carla Patricia
Flores Fernández, José Miguel
Lagunes Quintanilla, Rodolfo Esteban
Saines, Edgar Castro
Ramírez Rodríguez, Patricia Berenice
Herrera Rodríguez, Sara Elisa
Gutiérrez, Rodolfo Hernández
Ortega, Abel Gutiérrez
Álvarez, Ángel H.
author_facet Martínez Velázquez, Moisés
Barragán Álvarez, Carla Patricia
Flores Fernández, José Miguel
Lagunes Quintanilla, Rodolfo Esteban
Saines, Edgar Castro
Ramírez Rodríguez, Patricia Berenice
Herrera Rodríguez, Sara Elisa
Gutiérrez, Rodolfo Hernández
Ortega, Abel Gutiérrez
Álvarez, Ángel H.
author_sort Martínez Velázquez, Moisés
collection PubMed
description Rhipicephalus (Boophilus) microplus ticks are obligatory hematophagous ectoparasites of cattle and act as vectors for disease-causing microorganisms. Conventional tick control is based on the application of chemical acaricides; however, their uncontrolled use has increased resistant tick populations, as well as food and environmental contamination. Alternative immunological tick control has shown to be partially effective. Therefore, there is a need to characterize novel antigens in order to improve immunological protection. The aim of this work was to evaluate Cys-loop receptors as vaccine candidates. N-terminal domains of a glutamate receptor and of a glycine-like receptor were recombinantly produced in Escherichia coli. Groups of BALB/c mice were independently immunized with four doses of each recombinant protein emulsified with Freund’s adjuvant. Both vaccine candidates were immunogenic in mice as demonstrated by western blot analysis. Next, recombinant proteins were independently formulated with the adjuvant Montanide ISA 50 V2 and evaluated in cattle infested with Rhipicephalus microplus tick larvae. Groups of three European crossbred calves were immunized with three doses of each adjuvanted protein. ELISA test was used to evaluate the IgG immune response elicited against the recombinant proteins. Results showed that vaccine candidates generated a moderate humoral response on vaccinated cattle. Vaccination significantly affected the number of engorged adult female ticks, having no significant effects on tick weight, egg weight and egg fertility values. Vaccine efficacies of 33% and 25% were calculated for the glutamate receptor and the glycine-like receptor, respectively.
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spelling pubmed-84478682021-10-13 Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation Martínez Velázquez, Moisés Barragán Álvarez, Carla Patricia Flores Fernández, José Miguel Lagunes Quintanilla, Rodolfo Esteban Saines, Edgar Castro Ramírez Rodríguez, Patricia Berenice Herrera Rodríguez, Sara Elisa Gutiérrez, Rodolfo Hernández Ortega, Abel Gutiérrez Álvarez, Ángel H. Parasite Research Article Rhipicephalus (Boophilus) microplus ticks are obligatory hematophagous ectoparasites of cattle and act as vectors for disease-causing microorganisms. Conventional tick control is based on the application of chemical acaricides; however, their uncontrolled use has increased resistant tick populations, as well as food and environmental contamination. Alternative immunological tick control has shown to be partially effective. Therefore, there is a need to characterize novel antigens in order to improve immunological protection. The aim of this work was to evaluate Cys-loop receptors as vaccine candidates. N-terminal domains of a glutamate receptor and of a glycine-like receptor were recombinantly produced in Escherichia coli. Groups of BALB/c mice were independently immunized with four doses of each recombinant protein emulsified with Freund’s adjuvant. Both vaccine candidates were immunogenic in mice as demonstrated by western blot analysis. Next, recombinant proteins were independently formulated with the adjuvant Montanide ISA 50 V2 and evaluated in cattle infested with Rhipicephalus microplus tick larvae. Groups of three European crossbred calves were immunized with three doses of each adjuvanted protein. ELISA test was used to evaluate the IgG immune response elicited against the recombinant proteins. Results showed that vaccine candidates generated a moderate humoral response on vaccinated cattle. Vaccination significantly affected the number of engorged adult female ticks, having no significant effects on tick weight, egg weight and egg fertility values. Vaccine efficacies of 33% and 25% were calculated for the glutamate receptor and the glycine-like receptor, respectively. EDP Sciences 2021-09-14 /pmc/articles/PMC8447868/ /pubmed/34533454 http://dx.doi.org/10.1051/parasite/2021064 Text en © M.M. Velázquez et al., published by EDP Sciences, 2021 https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Martínez Velázquez, Moisés
Barragán Álvarez, Carla Patricia
Flores Fernández, José Miguel
Lagunes Quintanilla, Rodolfo Esteban
Saines, Edgar Castro
Ramírez Rodríguez, Patricia Berenice
Herrera Rodríguez, Sara Elisa
Gutiérrez, Rodolfo Hernández
Ortega, Abel Gutiérrez
Álvarez, Ángel H.
Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation
title Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation
title_full Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation
title_fullStr Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation
title_full_unstemmed Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation
title_short Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation
title_sort immunoprotection evaluation of the recombinant n-terminal domain of cys-loop receptors against rhipicephalus (boophilus) microplus tick infestation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447868/
https://www.ncbi.nlm.nih.gov/pubmed/34533454
http://dx.doi.org/10.1051/parasite/2021064
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