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Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation
Rhipicephalus (Boophilus) microplus ticks are obligatory hematophagous ectoparasites of cattle and act as vectors for disease-causing microorganisms. Conventional tick control is based on the application of chemical acaricides; however, their uncontrolled use has increased resistant tick populations...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
EDP Sciences
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447868/ https://www.ncbi.nlm.nih.gov/pubmed/34533454 http://dx.doi.org/10.1051/parasite/2021064 |
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author | Martínez Velázquez, Moisés Barragán Álvarez, Carla Patricia Flores Fernández, José Miguel Lagunes Quintanilla, Rodolfo Esteban Saines, Edgar Castro Ramírez Rodríguez, Patricia Berenice Herrera Rodríguez, Sara Elisa Gutiérrez, Rodolfo Hernández Ortega, Abel Gutiérrez Álvarez, Ángel H. |
author_facet | Martínez Velázquez, Moisés Barragán Álvarez, Carla Patricia Flores Fernández, José Miguel Lagunes Quintanilla, Rodolfo Esteban Saines, Edgar Castro Ramírez Rodríguez, Patricia Berenice Herrera Rodríguez, Sara Elisa Gutiérrez, Rodolfo Hernández Ortega, Abel Gutiérrez Álvarez, Ángel H. |
author_sort | Martínez Velázquez, Moisés |
collection | PubMed |
description | Rhipicephalus (Boophilus) microplus ticks are obligatory hematophagous ectoparasites of cattle and act as vectors for disease-causing microorganisms. Conventional tick control is based on the application of chemical acaricides; however, their uncontrolled use has increased resistant tick populations, as well as food and environmental contamination. Alternative immunological tick control has shown to be partially effective. Therefore, there is a need to characterize novel antigens in order to improve immunological protection. The aim of this work was to evaluate Cys-loop receptors as vaccine candidates. N-terminal domains of a glutamate receptor and of a glycine-like receptor were recombinantly produced in Escherichia coli. Groups of BALB/c mice were independently immunized with four doses of each recombinant protein emulsified with Freund’s adjuvant. Both vaccine candidates were immunogenic in mice as demonstrated by western blot analysis. Next, recombinant proteins were independently formulated with the adjuvant Montanide ISA 50 V2 and evaluated in cattle infested with Rhipicephalus microplus tick larvae. Groups of three European crossbred calves were immunized with three doses of each adjuvanted protein. ELISA test was used to evaluate the IgG immune response elicited against the recombinant proteins. Results showed that vaccine candidates generated a moderate humoral response on vaccinated cattle. Vaccination significantly affected the number of engorged adult female ticks, having no significant effects on tick weight, egg weight and egg fertility values. Vaccine efficacies of 33% and 25% were calculated for the glutamate receptor and the glycine-like receptor, respectively. |
format | Online Article Text |
id | pubmed-8447868 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | EDP Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-84478682021-10-13 Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation Martínez Velázquez, Moisés Barragán Álvarez, Carla Patricia Flores Fernández, José Miguel Lagunes Quintanilla, Rodolfo Esteban Saines, Edgar Castro Ramírez Rodríguez, Patricia Berenice Herrera Rodríguez, Sara Elisa Gutiérrez, Rodolfo Hernández Ortega, Abel Gutiérrez Álvarez, Ángel H. Parasite Research Article Rhipicephalus (Boophilus) microplus ticks are obligatory hematophagous ectoparasites of cattle and act as vectors for disease-causing microorganisms. Conventional tick control is based on the application of chemical acaricides; however, their uncontrolled use has increased resistant tick populations, as well as food and environmental contamination. Alternative immunological tick control has shown to be partially effective. Therefore, there is a need to characterize novel antigens in order to improve immunological protection. The aim of this work was to evaluate Cys-loop receptors as vaccine candidates. N-terminal domains of a glutamate receptor and of a glycine-like receptor were recombinantly produced in Escherichia coli. Groups of BALB/c mice were independently immunized with four doses of each recombinant protein emulsified with Freund’s adjuvant. Both vaccine candidates were immunogenic in mice as demonstrated by western blot analysis. Next, recombinant proteins were independently formulated with the adjuvant Montanide ISA 50 V2 and evaluated in cattle infested with Rhipicephalus microplus tick larvae. Groups of three European crossbred calves were immunized with three doses of each adjuvanted protein. ELISA test was used to evaluate the IgG immune response elicited against the recombinant proteins. Results showed that vaccine candidates generated a moderate humoral response on vaccinated cattle. Vaccination significantly affected the number of engorged adult female ticks, having no significant effects on tick weight, egg weight and egg fertility values. Vaccine efficacies of 33% and 25% were calculated for the glutamate receptor and the glycine-like receptor, respectively. EDP Sciences 2021-09-14 /pmc/articles/PMC8447868/ /pubmed/34533454 http://dx.doi.org/10.1051/parasite/2021064 Text en © M.M. Velázquez et al., published by EDP Sciences, 2021 https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0 (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Martínez Velázquez, Moisés Barragán Álvarez, Carla Patricia Flores Fernández, José Miguel Lagunes Quintanilla, Rodolfo Esteban Saines, Edgar Castro Ramírez Rodríguez, Patricia Berenice Herrera Rodríguez, Sara Elisa Gutiérrez, Rodolfo Hernández Ortega, Abel Gutiérrez Álvarez, Ángel H. Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation |
title | Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation |
title_full | Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation |
title_fullStr | Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation |
title_full_unstemmed | Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation |
title_short | Immunoprotection evaluation of the recombinant N-terminal domain of Cys-loop receptors against Rhipicephalus (Boophilus) microplus tick infestation |
title_sort | immunoprotection evaluation of the recombinant n-terminal domain of cys-loop receptors against rhipicephalus (boophilus) microplus tick infestation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8447868/ https://www.ncbi.nlm.nih.gov/pubmed/34533454 http://dx.doi.org/10.1051/parasite/2021064 |
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