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Suppression of ANT2 by miR-137 Inhibits Prostate Tumorigenesis

Prostate cancer (PCa) is a serious disease that affects men’s health. To date, no effective and long-lasting treatment option for this condition is available in clinical practice. ANT2 is highly expressed in a variety of hormone-related cancers, but its relationship and regulatory mechanism with PCa...

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Autores principales: Zhang, Heyuan, Chen, Nanhui, Deng, Zhihai, Mai, Yang, Deng, Limin, Chen, Guo, Li, Yutong, Pan, Bin, Zhong, Weifeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448070/
https://www.ncbi.nlm.nih.gov/pubmed/34539732
http://dx.doi.org/10.3389/fgene.2021.687236
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author Zhang, Heyuan
Chen, Nanhui
Deng, Zhihai
Mai, Yang
Deng, Limin
Chen, Guo
Li, Yutong
Pan, Bin
Zhong, Weifeng
author_facet Zhang, Heyuan
Chen, Nanhui
Deng, Zhihai
Mai, Yang
Deng, Limin
Chen, Guo
Li, Yutong
Pan, Bin
Zhong, Weifeng
author_sort Zhang, Heyuan
collection PubMed
description Prostate cancer (PCa) is a serious disease that affects men’s health. To date, no effective and long-lasting treatment option for this condition is available in clinical practice. ANT2 is highly expressed in a variety of hormone-related cancers, but its relationship and regulatory mechanism with PCa are unclear. In this study, we found that ANT2 expression was significantly upregulated in PCa tissues relative to control samples. Genetic knockdown of ANT2 effectively inhibited, while overexpression promoted, proliferation, migration, and invasion of PCa cells. In addition, miR-137 expression was reduced in prostate cancer tissues relative to control tissues. We identified a regulatory site for miR-137 in the 3′-UTR of ANT2 mRNA; luciferase reporter assays indicated that ANT2 is a direct target gene for miR-137. Transfecting cells with miR-137 mimics and/or an ANT2-encoding plasmid revealed that ANT2 promotes proliferation, migration, and invasion of PCa, whereas co-expression of miR-137 mimics inhibited these behaviors. These observations suggest that miR-137 mimics inhibit development of PCa by antagonizing expression of ANT2. Furthermore, tumorigenic assays in nude mice showed that miR-137 inhibitors abolished the inhibitory effect of ANT2 knockdown on PCa tumor growth. Collectively, our findings suggest that ANT2, a target gene of miR-137, is intimately involved in development of PCa, providing new evidence for the mechanism underlying pathogenesis of PCa as well as new options for targeted therapy.
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spelling pubmed-84480702021-09-18 Suppression of ANT2 by miR-137 Inhibits Prostate Tumorigenesis Zhang, Heyuan Chen, Nanhui Deng, Zhihai Mai, Yang Deng, Limin Chen, Guo Li, Yutong Pan, Bin Zhong, Weifeng Front Genet Genetics Prostate cancer (PCa) is a serious disease that affects men’s health. To date, no effective and long-lasting treatment option for this condition is available in clinical practice. ANT2 is highly expressed in a variety of hormone-related cancers, but its relationship and regulatory mechanism with PCa are unclear. In this study, we found that ANT2 expression was significantly upregulated in PCa tissues relative to control samples. Genetic knockdown of ANT2 effectively inhibited, while overexpression promoted, proliferation, migration, and invasion of PCa cells. In addition, miR-137 expression was reduced in prostate cancer tissues relative to control tissues. We identified a regulatory site for miR-137 in the 3′-UTR of ANT2 mRNA; luciferase reporter assays indicated that ANT2 is a direct target gene for miR-137. Transfecting cells with miR-137 mimics and/or an ANT2-encoding plasmid revealed that ANT2 promotes proliferation, migration, and invasion of PCa, whereas co-expression of miR-137 mimics inhibited these behaviors. These observations suggest that miR-137 mimics inhibit development of PCa by antagonizing expression of ANT2. Furthermore, tumorigenic assays in nude mice showed that miR-137 inhibitors abolished the inhibitory effect of ANT2 knockdown on PCa tumor growth. Collectively, our findings suggest that ANT2, a target gene of miR-137, is intimately involved in development of PCa, providing new evidence for the mechanism underlying pathogenesis of PCa as well as new options for targeted therapy. Frontiers Media S.A. 2021-09-03 /pmc/articles/PMC8448070/ /pubmed/34539732 http://dx.doi.org/10.3389/fgene.2021.687236 Text en Copyright © 2021 Zhang, Chen, Deng, Mai, Deng, Chen, Li, Pan and Zhong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Zhang, Heyuan
Chen, Nanhui
Deng, Zhihai
Mai, Yang
Deng, Limin
Chen, Guo
Li, Yutong
Pan, Bin
Zhong, Weifeng
Suppression of ANT2 by miR-137 Inhibits Prostate Tumorigenesis
title Suppression of ANT2 by miR-137 Inhibits Prostate Tumorigenesis
title_full Suppression of ANT2 by miR-137 Inhibits Prostate Tumorigenesis
title_fullStr Suppression of ANT2 by miR-137 Inhibits Prostate Tumorigenesis
title_full_unstemmed Suppression of ANT2 by miR-137 Inhibits Prostate Tumorigenesis
title_short Suppression of ANT2 by miR-137 Inhibits Prostate Tumorigenesis
title_sort suppression of ant2 by mir-137 inhibits prostate tumorigenesis
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448070/
https://www.ncbi.nlm.nih.gov/pubmed/34539732
http://dx.doi.org/10.3389/fgene.2021.687236
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