Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs

Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials...

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Detalles Bibliográficos
Autores principales: Grupi, Asaf, Shapira, Zehavit, Yudovich, Shimon, Degani-Katzav, Nurit, Weiss, Shimon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448295/
https://www.ncbi.nlm.nih.gov/pubmed/34568861
http://dx.doi.org/10.1016/j.bpr.2021.100007
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author Grupi, Asaf
Shapira, Zehavit
Yudovich, Shimon
Degani-Katzav, Nurit
Weiss, Shimon
author_facet Grupi, Asaf
Shapira, Zehavit
Yudovich, Shimon
Degani-Katzav, Nurit
Weiss, Shimon
author_sort Grupi, Asaf
collection PubMed
description Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABA(A) antibodies allowed for site-specific membrane potential measurements from postsynaptic sites.
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spelling pubmed-84482952021-09-22 Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs Grupi, Asaf Shapira, Zehavit Yudovich, Shimon Degani-Katzav, Nurit Weiss, Shimon Biophys Rep (N Y) Report Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABA(A) antibodies allowed for site-specific membrane potential measurements from postsynaptic sites. Elsevier 2021-07-24 /pmc/articles/PMC8448295/ /pubmed/34568861 http://dx.doi.org/10.1016/j.bpr.2021.100007 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Report
Grupi, Asaf
Shapira, Zehavit
Yudovich, Shimon
Degani-Katzav, Nurit
Weiss, Shimon
Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs
title Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs
title_full Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs
title_fullStr Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs
title_full_unstemmed Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs
title_short Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs
title_sort point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448295/
https://www.ncbi.nlm.nih.gov/pubmed/34568861
http://dx.doi.org/10.1016/j.bpr.2021.100007
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