High-throughput characterization of photocrosslinker-bearing ion channel variants to map residues critical for function and pharmacology

Incorporation of noncanonical amino acids (ncAAs) can endow proteins with novel functionalities, such as crosslinking or fluorescence. In ion channels, the function of these variants can be studied with great precision using standard electrophysiology, but this approach is typically labor intensive...

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Detalles Bibliográficos
Autores principales: Braun, Nina, Friis, Søren, Ihling, Christian, Sinz, Andrea, Andersen, Jacob, Pless, Stephan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Methods and Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448361/
https://www.ncbi.nlm.nih.gov/pubmed/34491979
http://dx.doi.org/10.1371/journal.pbio.3001321

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448361/
https://www.ncbi.nlm.nih.gov/pubmed/34491979
http://dx.doi.org/10.1371/journal.pbio.3001321

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