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Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi

BACKGROUND AND AIM: There has been continuous effort to search for alternative medicinal plants that are applicable to ameliorate viral disease on shrimp pond. This study aimed to examine the effect of Acanthus ilicifolius leaf extract on clinical symptoms and non-specific immune response of black t...

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Autores principales: Saptiani, Gina, Prayitno, Slamet Budi, Anggarawati, Sari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Veterinary World 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448654/
https://www.ncbi.nlm.nih.gov/pubmed/34566350
http://dx.doi.org/10.14202/vetworld.2021.2282-2289
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author Saptiani, Gina
Prayitno, Slamet Budi
Anggarawati, Sari
author_facet Saptiani, Gina
Prayitno, Slamet Budi
Anggarawati, Sari
author_sort Saptiani, Gina
collection PubMed
description BACKGROUND AND AIM: There has been continuous effort to search for alternative medicinal plants that are applicable to ameliorate viral disease on shrimp pond. This study aimed to examine the effect of Acanthus ilicifolius leaf extract on clinical symptoms and non-specific immune response of black tiger shrimp (Penaeus monodon). MATERIALS AND METHODS: A total of 330 shrimps were equally assigned into three extract forms (crude extract, ethyl acetate extract, and n-butanol extract, respectively) in which three levels were provided for each extract. Negative control (without leaf extract) and positive control (with oxytetracycline at 0.05 mg/mL) were used, giving a total of 11 experimental treatments. RESULTS: The results showed that shrimps induced into all form of leaf extracts had significantly higher survival rates, clinical symptoms, and pathological anatomy than those negative control (C−) and positive control (C+). Total hemocyte cells, granulocytes, percentage of phagocytic, and prophenoloxidase activity were similar among leaf extract treatments (p>0.05), but those groups were significantly higher than those of C− and C+ (p<0.05). CONCLUSION: n-butanol leaf extract at 300 mg/L is suggested to be the most effective treatment since it showed the highest efficacy on the parameters observed. Thus, it is possible to use the leaf extract of A. ilicifolius on-farm as a strategy to enhance bacterial disease resistance and prevent mortality.
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spelling pubmed-84486542021-09-24 Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi Saptiani, Gina Prayitno, Slamet Budi Anggarawati, Sari Vet World Research Article BACKGROUND AND AIM: There has been continuous effort to search for alternative medicinal plants that are applicable to ameliorate viral disease on shrimp pond. This study aimed to examine the effect of Acanthus ilicifolius leaf extract on clinical symptoms and non-specific immune response of black tiger shrimp (Penaeus monodon). MATERIALS AND METHODS: A total of 330 shrimps were equally assigned into three extract forms (crude extract, ethyl acetate extract, and n-butanol extract, respectively) in which three levels were provided for each extract. Negative control (without leaf extract) and positive control (with oxytetracycline at 0.05 mg/mL) were used, giving a total of 11 experimental treatments. RESULTS: The results showed that shrimps induced into all form of leaf extracts had significantly higher survival rates, clinical symptoms, and pathological anatomy than those negative control (C−) and positive control (C+). Total hemocyte cells, granulocytes, percentage of phagocytic, and prophenoloxidase activity were similar among leaf extract treatments (p>0.05), but those groups were significantly higher than those of C− and C+ (p<0.05). CONCLUSION: n-butanol leaf extract at 300 mg/L is suggested to be the most effective treatment since it showed the highest efficacy on the parameters observed. Thus, it is possible to use the leaf extract of A. ilicifolius on-farm as a strategy to enhance bacterial disease resistance and prevent mortality. Veterinary World 2021-08 2021-08-31 /pmc/articles/PMC8448654/ /pubmed/34566350 http://dx.doi.org/10.14202/vetworld.2021.2282-2289 Text en Copyright: © Saptiani, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Saptiani, Gina
Prayitno, Slamet Budi
Anggarawati, Sari
Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi
title Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi
title_full Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi
title_fullStr Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi
title_full_unstemmed Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi
title_short Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi
title_sort effect of mangrove leaf extract (acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (penaeus monodon) challenged with vibrio harveyi
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448654/
https://www.ncbi.nlm.nih.gov/pubmed/34566350
http://dx.doi.org/10.14202/vetworld.2021.2282-2289
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