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Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi
BACKGROUND AND AIM: There has been continuous effort to search for alternative medicinal plants that are applicable to ameliorate viral disease on shrimp pond. This study aimed to examine the effect of Acanthus ilicifolius leaf extract on clinical symptoms and non-specific immune response of black t...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Veterinary World
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448654/ https://www.ncbi.nlm.nih.gov/pubmed/34566350 http://dx.doi.org/10.14202/vetworld.2021.2282-2289 |
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author | Saptiani, Gina Prayitno, Slamet Budi Anggarawati, Sari |
author_facet | Saptiani, Gina Prayitno, Slamet Budi Anggarawati, Sari |
author_sort | Saptiani, Gina |
collection | PubMed |
description | BACKGROUND AND AIM: There has been continuous effort to search for alternative medicinal plants that are applicable to ameliorate viral disease on shrimp pond. This study aimed to examine the effect of Acanthus ilicifolius leaf extract on clinical symptoms and non-specific immune response of black tiger shrimp (Penaeus monodon). MATERIALS AND METHODS: A total of 330 shrimps were equally assigned into three extract forms (crude extract, ethyl acetate extract, and n-butanol extract, respectively) in which three levels were provided for each extract. Negative control (without leaf extract) and positive control (with oxytetracycline at 0.05 mg/mL) were used, giving a total of 11 experimental treatments. RESULTS: The results showed that shrimps induced into all form of leaf extracts had significantly higher survival rates, clinical symptoms, and pathological anatomy than those negative control (C−) and positive control (C+). Total hemocyte cells, granulocytes, percentage of phagocytic, and prophenoloxidase activity were similar among leaf extract treatments (p>0.05), but those groups were significantly higher than those of C− and C+ (p<0.05). CONCLUSION: n-butanol leaf extract at 300 mg/L is suggested to be the most effective treatment since it showed the highest efficacy on the parameters observed. Thus, it is possible to use the leaf extract of A. ilicifolius on-farm as a strategy to enhance bacterial disease resistance and prevent mortality. |
format | Online Article Text |
id | pubmed-8448654 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-84486542021-09-24 Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi Saptiani, Gina Prayitno, Slamet Budi Anggarawati, Sari Vet World Research Article BACKGROUND AND AIM: There has been continuous effort to search for alternative medicinal plants that are applicable to ameliorate viral disease on shrimp pond. This study aimed to examine the effect of Acanthus ilicifolius leaf extract on clinical symptoms and non-specific immune response of black tiger shrimp (Penaeus monodon). MATERIALS AND METHODS: A total of 330 shrimps were equally assigned into three extract forms (crude extract, ethyl acetate extract, and n-butanol extract, respectively) in which three levels were provided for each extract. Negative control (without leaf extract) and positive control (with oxytetracycline at 0.05 mg/mL) were used, giving a total of 11 experimental treatments. RESULTS: The results showed that shrimps induced into all form of leaf extracts had significantly higher survival rates, clinical symptoms, and pathological anatomy than those negative control (C−) and positive control (C+). Total hemocyte cells, granulocytes, percentage of phagocytic, and prophenoloxidase activity were similar among leaf extract treatments (p>0.05), but those groups were significantly higher than those of C− and C+ (p<0.05). CONCLUSION: n-butanol leaf extract at 300 mg/L is suggested to be the most effective treatment since it showed the highest efficacy on the parameters observed. Thus, it is possible to use the leaf extract of A. ilicifolius on-farm as a strategy to enhance bacterial disease resistance and prevent mortality. Veterinary World 2021-08 2021-08-31 /pmc/articles/PMC8448654/ /pubmed/34566350 http://dx.doi.org/10.14202/vetworld.2021.2282-2289 Text en Copyright: © Saptiani, et al. https://creativecommons.org/licenses/by/4.0/Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Saptiani, Gina Prayitno, Slamet Budi Anggarawati, Sari Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi |
title | Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi |
title_full | Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi |
title_fullStr | Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi |
title_full_unstemmed | Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi |
title_short | Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi |
title_sort | effect of mangrove leaf extract (acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (penaeus monodon) challenged with vibrio harveyi |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448654/ https://www.ncbi.nlm.nih.gov/pubmed/34566350 http://dx.doi.org/10.14202/vetworld.2021.2282-2289 |
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