Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs

Extracellular vesicles (EVs) are mediators of the immune system response. Encapsulated in EVs, microRNAs can be transferred between cancer and immune cells. To define the potential effects of EVs originated from squamous cell carcinoma cells on immune system response, we performed microRNA profiling...

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Autores principales: de Paula Silva, Elisangela, Marti, Luciana Cavalheiro, Andreghetto, Flávia Maziero, de Sales, Romário Oliveira, Hoberman, Martin, dos Santos Dias, Bárbara, Diniz, Larissa Figueiredo Alves, dos Santos, Alessandro Marins, Moyses, Raquel Ajub, Curioni, Otávio Alberto, Lopez, Rossana Veronica Mendoza, Nunes, Fabio Daumas, Tajara, Eloiza Helena, Severino, Patricia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448882/
https://www.ncbi.nlm.nih.gov/pubmed/34535708
http://dx.doi.org/10.1038/s41598-021-97753-y
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author de Paula Silva, Elisangela
Marti, Luciana Cavalheiro
Andreghetto, Flávia Maziero
de Sales, Romário Oliveira
Hoberman, Martin
dos Santos Dias, Bárbara
Diniz, Larissa Figueiredo Alves
dos Santos, Alessandro Marins
Moyses, Raquel Ajub
Curioni, Otávio Alberto
Lopez, Rossana Veronica Mendoza
Nunes, Fabio Daumas
Tajara, Eloiza Helena
Severino, Patricia
author_facet de Paula Silva, Elisangela
Marti, Luciana Cavalheiro
Andreghetto, Flávia Maziero
de Sales, Romário Oliveira
Hoberman, Martin
dos Santos Dias, Bárbara
Diniz, Larissa Figueiredo Alves
dos Santos, Alessandro Marins
Moyses, Raquel Ajub
Curioni, Otávio Alberto
Lopez, Rossana Veronica Mendoza
Nunes, Fabio Daumas
Tajara, Eloiza Helena
Severino, Patricia
author_sort de Paula Silva, Elisangela
collection PubMed
description Extracellular vesicles (EVs) are mediators of the immune system response. Encapsulated in EVs, microRNAs can be transferred between cancer and immune cells. To define the potential effects of EVs originated from squamous cell carcinoma cells on immune system response, we performed microRNA profiling of EVs released from two distinct cell lines and treated dendritic cells derived from circulating monocytes (mono-DCs) with these EVs. We confirmed the internalization of EVs by mono-DCs and the down-regulation of microRNA mRNA targets in treated mono-DCs. Differences in surface markers of dendritic cells cultivated in the presence of EVs indicated that their content disrupts the maturation process. Additionally, microRNAs known to interfere with dendritic cell function, and detected in EVs, matched microRNAs from squamous cell carcinoma patients’ plasma: miR-17-5p in oropharyngeal squamous cell carcinoma, miR-21 in oral squamous cell carcinoma, miR-16, miR-24, and miR-181a circulating in both oral and oropharyngeal squamous cell carcinoma, and miR-23b, which has not been previously described in plasma of head and neck squamous cell carcinoma, was found in plasma from patients with these cancer subtypes. This study contributes with insights on EVs in signaling between cancer and immune cells in squamous cell carcinoma of the head and neck.
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spelling pubmed-84488822021-09-21 Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs de Paula Silva, Elisangela Marti, Luciana Cavalheiro Andreghetto, Flávia Maziero de Sales, Romário Oliveira Hoberman, Martin dos Santos Dias, Bárbara Diniz, Larissa Figueiredo Alves dos Santos, Alessandro Marins Moyses, Raquel Ajub Curioni, Otávio Alberto Lopez, Rossana Veronica Mendoza Nunes, Fabio Daumas Tajara, Eloiza Helena Severino, Patricia Sci Rep Article Extracellular vesicles (EVs) are mediators of the immune system response. Encapsulated in EVs, microRNAs can be transferred between cancer and immune cells. To define the potential effects of EVs originated from squamous cell carcinoma cells on immune system response, we performed microRNA profiling of EVs released from two distinct cell lines and treated dendritic cells derived from circulating monocytes (mono-DCs) with these EVs. We confirmed the internalization of EVs by mono-DCs and the down-regulation of microRNA mRNA targets in treated mono-DCs. Differences in surface markers of dendritic cells cultivated in the presence of EVs indicated that their content disrupts the maturation process. Additionally, microRNAs known to interfere with dendritic cell function, and detected in EVs, matched microRNAs from squamous cell carcinoma patients’ plasma: miR-17-5p in oropharyngeal squamous cell carcinoma, miR-21 in oral squamous cell carcinoma, miR-16, miR-24, and miR-181a circulating in both oral and oropharyngeal squamous cell carcinoma, and miR-23b, which has not been previously described in plasma of head and neck squamous cell carcinoma, was found in plasma from patients with these cancer subtypes. This study contributes with insights on EVs in signaling between cancer and immune cells in squamous cell carcinoma of the head and neck. Nature Publishing Group UK 2021-09-17 /pmc/articles/PMC8448882/ /pubmed/34535708 http://dx.doi.org/10.1038/s41598-021-97753-y Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
de Paula Silva, Elisangela
Marti, Luciana Cavalheiro
Andreghetto, Flávia Maziero
de Sales, Romário Oliveira
Hoberman, Martin
dos Santos Dias, Bárbara
Diniz, Larissa Figueiredo Alves
dos Santos, Alessandro Marins
Moyses, Raquel Ajub
Curioni, Otávio Alberto
Lopez, Rossana Veronica Mendoza
Nunes, Fabio Daumas
Tajara, Eloiza Helena
Severino, Patricia
Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs
title Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs
title_full Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs
title_fullStr Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs
title_full_unstemmed Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs
title_short Extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma microRNAs
title_sort extracellular vesicles cargo from head and neck cancer cell lines disrupt dendritic cells function and match plasma micrornas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448882/
https://www.ncbi.nlm.nih.gov/pubmed/34535708
http://dx.doi.org/10.1038/s41598-021-97753-y
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