A proteomic investigation of isogenic radiation resistant prostate cancer cell lines

To model the problem of radiation resistance in prostate cancer, cell lines mimicking a clinical course of conventionally fractionated or hypofractionated radiotherapy have been generated. Proteomic analysis of radiation resistant and radiosensitive DU145 prostate cancer cells detected 4410 proteins...

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Autores principales: Kurganovs, Natalie, Wang, Hanzhi, Huang, Xiaoyong, Ignatchenko, Vladimir, Macklin, Andrew, Khan, Shahbaz, Downes, Michelle R., Boutros, Paul C., Liu, Stanley K., Kislinger, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448965/
https://www.ncbi.nlm.nih.gov/pubmed/34152685
http://dx.doi.org/10.1002/prca.202100037
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author Kurganovs, Natalie
Wang, Hanzhi
Huang, Xiaoyong
Ignatchenko, Vladimir
Macklin, Andrew
Khan, Shahbaz
Downes, Michelle R.
Boutros, Paul C.
Liu, Stanley K.
Kislinger, Thomas
author_facet Kurganovs, Natalie
Wang, Hanzhi
Huang, Xiaoyong
Ignatchenko, Vladimir
Macklin, Andrew
Khan, Shahbaz
Downes, Michelle R.
Boutros, Paul C.
Liu, Stanley K.
Kislinger, Thomas
author_sort Kurganovs, Natalie
collection PubMed
description To model the problem of radiation resistance in prostate cancer, cell lines mimicking a clinical course of conventionally fractionated or hypofractionated radiotherapy have been generated. Proteomic analysis of radiation resistant and radiosensitive DU145 prostate cancer cells detected 4410 proteins. Over 400 proteins were differentially expressed across both radiation resistant cell lines and pathway analysis revealed enrichment in epithelial to mesenchymal transition, glycolysis and hypoxia. From the radiation resistant protein candidates, the cell surface protein CD44 was identified in the glycolysis and epithelial to mesenchymal transition pathways and may serve as a potential therapeutic target.
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spelling pubmed-84489652021-09-18 A proteomic investigation of isogenic radiation resistant prostate cancer cell lines Kurganovs, Natalie Wang, Hanzhi Huang, Xiaoyong Ignatchenko, Vladimir Macklin, Andrew Khan, Shahbaz Downes, Michelle R. Boutros, Paul C. Liu, Stanley K. Kislinger, Thomas Proteomics Clin Appl Research Articles To model the problem of radiation resistance in prostate cancer, cell lines mimicking a clinical course of conventionally fractionated or hypofractionated radiotherapy have been generated. Proteomic analysis of radiation resistant and radiosensitive DU145 prostate cancer cells detected 4410 proteins. Over 400 proteins were differentially expressed across both radiation resistant cell lines and pathway analysis revealed enrichment in epithelial to mesenchymal transition, glycolysis and hypoxia. From the radiation resistant protein candidates, the cell surface protein CD44 was identified in the glycolysis and epithelial to mesenchymal transition pathways and may serve as a potential therapeutic target. John Wiley and Sons Inc. 2021-06-30 2021-09 /pmc/articles/PMC8448965/ /pubmed/34152685 http://dx.doi.org/10.1002/prca.202100037 Text en © 2021 The Authors. Proteomics – Clinical Applications published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Kurganovs, Natalie
Wang, Hanzhi
Huang, Xiaoyong
Ignatchenko, Vladimir
Macklin, Andrew
Khan, Shahbaz
Downes, Michelle R.
Boutros, Paul C.
Liu, Stanley K.
Kislinger, Thomas
A proteomic investigation of isogenic radiation resistant prostate cancer cell lines
title A proteomic investigation of isogenic radiation resistant prostate cancer cell lines
title_full A proteomic investigation of isogenic radiation resistant prostate cancer cell lines
title_fullStr A proteomic investigation of isogenic radiation resistant prostate cancer cell lines
title_full_unstemmed A proteomic investigation of isogenic radiation resistant prostate cancer cell lines
title_short A proteomic investigation of isogenic radiation resistant prostate cancer cell lines
title_sort proteomic investigation of isogenic radiation resistant prostate cancer cell lines
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8448965/
https://www.ncbi.nlm.nih.gov/pubmed/34152685
http://dx.doi.org/10.1002/prca.202100037
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