Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature

Current protocols for storage of white blood cells (WBCs) rely on constant refrigeration. The protocol described below explains the preparation of a fixative combination saline (FCS) formulation, which allows fixation of human WBCs and lysis of red blood cells and platelets (at ambient temperature,...

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Autores principales: Chaurasia, Rajesh K., Shirsath, Kapil B., Sapra, Balvinder K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449125/
https://www.ncbi.nlm.nih.gov/pubmed/34568847
http://dx.doi.org/10.1016/j.xpro.2021.100834
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author Chaurasia, Rajesh K.
Shirsath, Kapil B.
Sapra, Balvinder K.
author_facet Chaurasia, Rajesh K.
Shirsath, Kapil B.
Sapra, Balvinder K.
author_sort Chaurasia, Rajesh K.
collection PubMed
description Current protocols for storage of white blood cells (WBCs) rely on constant refrigeration. The protocol described below explains the preparation of a fixative combination saline (FCS) formulation, which allows fixation of human WBCs and lysis of red blood cells and platelets (at ambient temperature, 4–35(o)C) in whole blood samples in one step. FCS can be used for storing and transporting blood at ambient temperatures for up to 4 months, without altering the nuclear morphology and genomic integrity of WBCs.
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spelling pubmed-84491252021-09-24 Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature Chaurasia, Rajesh K. Shirsath, Kapil B. Sapra, Balvinder K. STAR Protoc Protocol Current protocols for storage of white blood cells (WBCs) rely on constant refrigeration. The protocol described below explains the preparation of a fixative combination saline (FCS) formulation, which allows fixation of human WBCs and lysis of red blood cells and platelets (at ambient temperature, 4–35(o)C) in whole blood samples in one step. FCS can be used for storing and transporting blood at ambient temperatures for up to 4 months, without altering the nuclear morphology and genomic integrity of WBCs. Elsevier 2021-09-15 /pmc/articles/PMC8449125/ /pubmed/34568847 http://dx.doi.org/10.1016/j.xpro.2021.100834 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chaurasia, Rajesh K.
Shirsath, Kapil B.
Sapra, Balvinder K.
Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
title Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
title_full Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
title_fullStr Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
title_full_unstemmed Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
title_short Protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
title_sort protocol for one-step selective lysis of red blood cells and platelets with long-term preservation of white blood cells (human) at ambient temperature
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449125/
https://www.ncbi.nlm.nih.gov/pubmed/34568847
http://dx.doi.org/10.1016/j.xpro.2021.100834
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