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Single tracer-based protocol for broad-spectrum kinase profiling in live cells with NanoBRET

This protocol is used to profile the engagement of kinase inhibitors across nearly 200 kinases in a live-cell context. This protocol utilizes one single kinase tracer (NanoBRET(TM) Tracer K10) that operates quantitatively at four different concentrations. Minimizing the number of tracers offers a si...

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Detalles Bibliográficos
Autores principales:	Robers, Matthew B., Wilkinson, Jennifer M., Vasta, James D., Berger, Lena M., Berger, Benedict-Tilman, Knapp, Stefan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449129/ https://www.ncbi.nlm.nih.gov/pubmed/34568844 http://dx.doi.org/10.1016/j.xpro.2021.100822

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