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A mutation in budding yeast BRR6 affecting nuclear envelope insertion of the spindle pole body
BRR6 and BRL1 are two paralogs that encode transmembrane proteins of the nuclear envelope (NE) involved in membrane fluidity and nuclear pore complex biogenesis in organisms that undergo a closed mitosis. We show that mutation of a conserved cysteine in the intralumenal domain of Saccharomyces cerev...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449258/ https://www.ncbi.nlm.nih.gov/pubmed/34549174 http://dx.doi.org/10.17912/micropub.biology.000463 |
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author | Gardner, Jennifer M O'Toole, Eileen Jaspersen, Sue L |
author_facet | Gardner, Jennifer M O'Toole, Eileen Jaspersen, Sue L |
author_sort | Gardner, Jennifer M |
collection | PubMed |
description | BRR6 and BRL1 are two paralogs that encode transmembrane proteins of the nuclear envelope (NE) involved in membrane fluidity and nuclear pore complex biogenesis in organisms that undergo a closed mitosis. We show that mutation of a conserved cysteine in the intralumenal domain of Saccharomyces cerevisiae Brr6p results in a novel temperature sensitive allele, brr6-Y100H, that arrests growth due to defects in spindle formation. Analysis of brr6-Y100H cells by electron tomography and Brr6p localization by super-resolution imaging supports the idea that Brr6p is involved in insertion of the newly duplicated spindle pole body into the NE. |
format | Online Article Text |
id | pubmed-8449258 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-84492582021-09-20 A mutation in budding yeast BRR6 affecting nuclear envelope insertion of the spindle pole body Gardner, Jennifer M O'Toole, Eileen Jaspersen, Sue L MicroPubl Biol New Finding BRR6 and BRL1 are two paralogs that encode transmembrane proteins of the nuclear envelope (NE) involved in membrane fluidity and nuclear pore complex biogenesis in organisms that undergo a closed mitosis. We show that mutation of a conserved cysteine in the intralumenal domain of Saccharomyces cerevisiae Brr6p results in a novel temperature sensitive allele, brr6-Y100H, that arrests growth due to defects in spindle formation. Analysis of brr6-Y100H cells by electron tomography and Brr6p localization by super-resolution imaging supports the idea that Brr6p is involved in insertion of the newly duplicated spindle pole body into the NE. Caltech Library 2021-09-16 /pmc/articles/PMC8449258/ /pubmed/34549174 http://dx.doi.org/10.17912/micropub.biology.000463 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Finding Gardner, Jennifer M O'Toole, Eileen Jaspersen, Sue L A mutation in budding yeast BRR6 affecting nuclear envelope insertion of the spindle pole body |
title | A mutation in budding yeast BRR6 affecting nuclear envelope insertion of the spindle pole body |
title_full | A mutation in budding yeast BRR6 affecting nuclear envelope insertion of the spindle pole body |
title_fullStr | A mutation in budding yeast BRR6 affecting nuclear envelope insertion of the spindle pole body |
title_full_unstemmed | A mutation in budding yeast BRR6 affecting nuclear envelope insertion of the spindle pole body |
title_short | A mutation in budding yeast BRR6 affecting nuclear envelope insertion of the spindle pole body |
title_sort | mutation in budding yeast brr6 affecting nuclear envelope insertion of the spindle pole body |
topic | New Finding |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449258/ https://www.ncbi.nlm.nih.gov/pubmed/34549174 http://dx.doi.org/10.17912/micropub.biology.000463 |
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