Improved GAL4 and Tet OFF drivers for C. elegans bipartite expression

The first generation of C. elegans GAL4 drivers for bipartite expression function less well than C. elegans tet ON/OFF, QF and LexA drivers. The main difference between the GAL4 drivers and the others is the absence of a flexible linker between the DNA binding and activation domain in the GAL4 const...

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Detalles Bibliográficos
Autor principal: Nonet, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
Materials and Reagents
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449259/
https://www.ncbi.nlm.nih.gov/pubmed/34549175
http://dx.doi.org/10.17912/micropub.biology.000438
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author Nonet, Michael
author_facet Nonet, Michael
author_sort Nonet, Michael
collection PubMed
description The first generation of C. elegans GAL4 drivers for bipartite expression function less well than C. elegans tet ON/OFF, QF and LexA drivers. The main difference between the GAL4 drivers and the others is the absence of a flexible linker between the DNA binding and activation domain in the GAL4 construct. Addition of a linker to a GAL4-QF construct increased driver potency, while adding linkers to a GAL4-VP64 driver was much less effective. Extending the linker region of the tetR-L-QF driver also increased activity of that driver. The new GAL4 driver makes GAL4/UAS bipartite system activity comparable to the other worm bipartite expression systems.
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spelling pubmed-84492592021-09-20 Improved GAL4 and Tet OFF drivers for C. elegans bipartite expression Nonet, Michael MicroPubl Biol Materials and Reagents The first generation of C. elegans GAL4 drivers for bipartite expression function less well than C. elegans tet ON/OFF, QF and LexA drivers. The main difference between the GAL4 drivers and the others is the absence of a flexible linker between the DNA binding and activation domain in the GAL4 construct. Addition of a linker to a GAL4-QF construct increased driver potency, while adding linkers to a GAL4-VP64 driver was much less effective. Extending the linker region of the tetR-L-QF driver also increased activity of that driver. The new GAL4 driver makes GAL4/UAS bipartite system activity comparable to the other worm bipartite expression systems. Caltech Library 2021-09-14 /pmc/articles/PMC8449259/ /pubmed/34549175 http://dx.doi.org/10.17912/micropub.biology.000438 Text en Copyright: © 2021 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Materials and Reagents
Nonet, Michael
Improved GAL4 and Tet OFF drivers for C. elegans bipartite expression
title Improved GAL4 and Tet OFF drivers for C. elegans bipartite expression
title_full Improved GAL4 and Tet OFF drivers for C. elegans bipartite expression
title_fullStr Improved GAL4 and Tet OFF drivers for C. elegans bipartite expression
title_full_unstemmed Improved GAL4 and Tet OFF drivers for C. elegans bipartite expression
title_short Improved GAL4 and Tet OFF drivers for C. elegans bipartite expression
title_sort improved gal4 and tet off drivers for c. elegans bipartite expression
topic Materials and Reagents
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449259/
https://www.ncbi.nlm.nih.gov/pubmed/34549175
http://dx.doi.org/10.17912/micropub.biology.000438
work_keys_str_mv AT nonetmichael improvedgal4andtetoffdriversforcelegansbipartiteexpression

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