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Efficacy of cyclic lipopeptides obtained from Bacillus subtilis to inhibit the growth of Microsporum canis isolated from cats

BACKGROUND AND AIM: Microsporum canis (M. canis) is a dermatophyte fungal pathogen that causes ringworms. Cats are considered to be a dominant reservoir host enabling M. canis transmission to humans. The concerns of dermatophyte resistance were raised among the usage of antifungal drugs to treat the...

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Autores principales: Tunsagool, Paiboon, Ploypetch, Sekkarin, Jaresitthikunchai, Janthima, Roytrakul, Sittiruk, Choowongkomon, Kiattawee, Rattanasrisomporn, Jatuporn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450251/
https://www.ncbi.nlm.nih.gov/pubmed/34585007
http://dx.doi.org/10.1016/j.heliyon.2021.e07980
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author Tunsagool, Paiboon
Ploypetch, Sekkarin
Jaresitthikunchai, Janthima
Roytrakul, Sittiruk
Choowongkomon, Kiattawee
Rattanasrisomporn, Jatuporn
author_facet Tunsagool, Paiboon
Ploypetch, Sekkarin
Jaresitthikunchai, Janthima
Roytrakul, Sittiruk
Choowongkomon, Kiattawee
Rattanasrisomporn, Jatuporn
author_sort Tunsagool, Paiboon
collection PubMed
description BACKGROUND AND AIM: Microsporum canis (M. canis) is a dermatophyte fungal pathogen that causes ringworms. Cats are considered to be a dominant reservoir host enabling M. canis transmission to humans. The concerns of dermatophyte resistance were raised among the usage of antifungal drugs to treat the ringworm. This study aimed to evaluate the fungal activity of cyclic lipopeptides (CLPs) obtained from Bacillus subtilis (B. subtilis) as an alternative method for the inhibition of M. canis growth. MATERIALS AND METHODS: The culture plate of M. canis from confirmed cats with ringworm infection was provided. The purification of CLP extract, fengycin, iturin A, and surfactin was carried out from B. subtilis by preparative thin-layer chromatography (PTLC) coupled with solid-phase extraction (SPE) methods. Half-maximal effective concentration (EC(50)) and agar well diffusion assays were performed to determine the efficacy of Bacillus CLP extract, fengycin, iturin A, and surfactin to inhibit the growth of M. canis isolated from cats. RESULTS: All purified Bacillus substances displayed antifungal activity to control the growth of M. canis when compared with 80% ethanol (control). EC(50) values for CLP extract, fengycin, iturin A, and surfactin were 0.23, 0.05, 0.17, and 0.08 mg/mL, respectively. In agar well diffusion assay, the ability of CLP extract, fengycin, iturin A, and surfactin on fungal inhibition had no statistically significant difference at 24 and 48 h after treatment (p < 0.05). However, CLP extract showed a statistically significant difference on M. canis inhibition at 62.21% followed by surfactin with 59.04% at 72 h after treatment. CONCLUSION: In vitro, Bacillus CLPs revealed an inhibitory effect on M. canis growth which is a zoonotic pathogen that causes ringworms. This study suggests an alternative approach to control the growth of M. canis using substances obtained from B. subtilis as a biomedicine agent with antifungal activity.
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spelling pubmed-84502512021-09-27 Efficacy of cyclic lipopeptides obtained from Bacillus subtilis to inhibit the growth of Microsporum canis isolated from cats Tunsagool, Paiboon Ploypetch, Sekkarin Jaresitthikunchai, Janthima Roytrakul, Sittiruk Choowongkomon, Kiattawee Rattanasrisomporn, Jatuporn Heliyon Research Article BACKGROUND AND AIM: Microsporum canis (M. canis) is a dermatophyte fungal pathogen that causes ringworms. Cats are considered to be a dominant reservoir host enabling M. canis transmission to humans. The concerns of dermatophyte resistance were raised among the usage of antifungal drugs to treat the ringworm. This study aimed to evaluate the fungal activity of cyclic lipopeptides (CLPs) obtained from Bacillus subtilis (B. subtilis) as an alternative method for the inhibition of M. canis growth. MATERIALS AND METHODS: The culture plate of M. canis from confirmed cats with ringworm infection was provided. The purification of CLP extract, fengycin, iturin A, and surfactin was carried out from B. subtilis by preparative thin-layer chromatography (PTLC) coupled with solid-phase extraction (SPE) methods. Half-maximal effective concentration (EC(50)) and agar well diffusion assays were performed to determine the efficacy of Bacillus CLP extract, fengycin, iturin A, and surfactin to inhibit the growth of M. canis isolated from cats. RESULTS: All purified Bacillus substances displayed antifungal activity to control the growth of M. canis when compared with 80% ethanol (control). EC(50) values for CLP extract, fengycin, iturin A, and surfactin were 0.23, 0.05, 0.17, and 0.08 mg/mL, respectively. In agar well diffusion assay, the ability of CLP extract, fengycin, iturin A, and surfactin on fungal inhibition had no statistically significant difference at 24 and 48 h after treatment (p < 0.05). However, CLP extract showed a statistically significant difference on M. canis inhibition at 62.21% followed by surfactin with 59.04% at 72 h after treatment. CONCLUSION: In vitro, Bacillus CLPs revealed an inhibitory effect on M. canis growth which is a zoonotic pathogen that causes ringworms. This study suggests an alternative approach to control the growth of M. canis using substances obtained from B. subtilis as a biomedicine agent with antifungal activity. Elsevier 2021-09-11 /pmc/articles/PMC8450251/ /pubmed/34585007 http://dx.doi.org/10.1016/j.heliyon.2021.e07980 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Tunsagool, Paiboon
Ploypetch, Sekkarin
Jaresitthikunchai, Janthima
Roytrakul, Sittiruk
Choowongkomon, Kiattawee
Rattanasrisomporn, Jatuporn
Efficacy of cyclic lipopeptides obtained from Bacillus subtilis to inhibit the growth of Microsporum canis isolated from cats
title Efficacy of cyclic lipopeptides obtained from Bacillus subtilis to inhibit the growth of Microsporum canis isolated from cats
title_full Efficacy of cyclic lipopeptides obtained from Bacillus subtilis to inhibit the growth of Microsporum canis isolated from cats
title_fullStr Efficacy of cyclic lipopeptides obtained from Bacillus subtilis to inhibit the growth of Microsporum canis isolated from cats
title_full_unstemmed Efficacy of cyclic lipopeptides obtained from Bacillus subtilis to inhibit the growth of Microsporum canis isolated from cats
title_short Efficacy of cyclic lipopeptides obtained from Bacillus subtilis to inhibit the growth of Microsporum canis isolated from cats
title_sort efficacy of cyclic lipopeptides obtained from bacillus subtilis to inhibit the growth of microsporum canis isolated from cats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450251/
https://www.ncbi.nlm.nih.gov/pubmed/34585007
http://dx.doi.org/10.1016/j.heliyon.2021.e07980
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