Isolation of mouse Kupffer cells for phenotypic and functional studies

Here, we provide detailed protocols for the isolation of mouse Kupffer cells – the liver-resident macrophages – for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted for the...

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Autores principales: Andreata, Francesco, Blériot, Camille, Di Lucia, Pietro, De Simone, Giorgia, Fumagalli, Valeria, Ficht, Xenia, Beccaria, Cristian Gabriel, Kuka, Mirela, Ginhoux, Florent, Iannacone, Matteo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450292/
https://www.ncbi.nlm.nih.gov/pubmed/34585164
http://dx.doi.org/10.1016/j.xpro.2021.100831
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author Andreata, Francesco
Blériot, Camille
Di Lucia, Pietro
De Simone, Giorgia
Fumagalli, Valeria
Ficht, Xenia
Beccaria, Cristian Gabriel
Kuka, Mirela
Ginhoux, Florent
Iannacone, Matteo
author_facet Andreata, Francesco
Blériot, Camille
Di Lucia, Pietro
De Simone, Giorgia
Fumagalli, Valeria
Ficht, Xenia
Beccaria, Cristian Gabriel
Kuka, Mirela
Ginhoux, Florent
Iannacone, Matteo
author_sort Andreata, Francesco
collection PubMed
description Here, we provide detailed protocols for the isolation of mouse Kupffer cells – the liver-resident macrophages – for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted for the isolation of other hepatic cell populations. For complete details on the use and execution of this protocol, please refer to De Simone et al. (2021).
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spelling pubmed-84502922021-09-27 Isolation of mouse Kupffer cells for phenotypic and functional studies Andreata, Francesco Blériot, Camille Di Lucia, Pietro De Simone, Giorgia Fumagalli, Valeria Ficht, Xenia Beccaria, Cristian Gabriel Kuka, Mirela Ginhoux, Florent Iannacone, Matteo STAR Protoc Protocol Here, we provide detailed protocols for the isolation of mouse Kupffer cells – the liver-resident macrophages – for phenotypic (e.g., via flow cytometry, mass cytometry, or RNA-sequencing) analyses or for functional experiments involving cell culture. The procedures presented can be adapted for the isolation of other hepatic cell populations. For complete details on the use and execution of this protocol, please refer to De Simone et al. (2021). Elsevier 2021-09-14 /pmc/articles/PMC8450292/ /pubmed/34585164 http://dx.doi.org/10.1016/j.xpro.2021.100831 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Andreata, Francesco
Blériot, Camille
Di Lucia, Pietro
De Simone, Giorgia
Fumagalli, Valeria
Ficht, Xenia
Beccaria, Cristian Gabriel
Kuka, Mirela
Ginhoux, Florent
Iannacone, Matteo
Isolation of mouse Kupffer cells for phenotypic and functional studies
title Isolation of mouse Kupffer cells for phenotypic and functional studies
title_full Isolation of mouse Kupffer cells for phenotypic and functional studies
title_fullStr Isolation of mouse Kupffer cells for phenotypic and functional studies
title_full_unstemmed Isolation of mouse Kupffer cells for phenotypic and functional studies
title_short Isolation of mouse Kupffer cells for phenotypic and functional studies
title_sort isolation of mouse kupffer cells for phenotypic and functional studies
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450292/
https://www.ncbi.nlm.nih.gov/pubmed/34585164
http://dx.doi.org/10.1016/j.xpro.2021.100831
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