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Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Early mechanistic studies

Human norovirus is the main cause of viral gastroenteritis, resulting annually in ∼ 700 million infections and 200,000 deaths, of whom most are children <5 years. Mouse norovirus-infected macrophages are the most widely used in vitro system to screen and characterize the antiviral effect of norov...

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Autores principales: Dycke, Jana Van, Rymenants, Jasper, Neyts, Johan, Rocha-Pereira, Joana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450984/
https://www.ncbi.nlm.nih.gov/pubmed/34525875
http://dx.doi.org/10.1177/20402066211025175
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author Dycke, Jana Van
Rymenants, Jasper
Neyts, Johan
Rocha-Pereira, Joana
author_facet Dycke, Jana Van
Rymenants, Jasper
Neyts, Johan
Rocha-Pereira, Joana
author_sort Dycke, Jana Van
collection PubMed
description Human norovirus is the main cause of viral gastroenteritis, resulting annually in ∼ 700 million infections and 200,000 deaths, of whom most are children <5 years. Mouse norovirus-infected macrophages are the most widely used in vitro system to screen and characterize the antiviral effect of norovirus-targeting small molecules. We have previously established antiviral assays using this system, identified novel inhibitors and performed additional studies in order to have a first insight into their mechanism of action. After the identification of novel small molecules with anti-norovirus activity (part 1 of this protocol), we here describe the logical next step which entails the generation of early information of their mode of action. This information together with a continuous improvement of the potency of compounds will contribute to the optimization of a compound class towards in vivo efficacy and a successful preclinical development.
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spelling pubmed-84509842021-09-21 Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Early mechanistic studies Dycke, Jana Van Rymenants, Jasper Neyts, Johan Rocha-Pereira, Joana Antivir Chem Chemother Protocol Human norovirus is the main cause of viral gastroenteritis, resulting annually in ∼ 700 million infections and 200,000 deaths, of whom most are children <5 years. Mouse norovirus-infected macrophages are the most widely used in vitro system to screen and characterize the antiviral effect of norovirus-targeting small molecules. We have previously established antiviral assays using this system, identified novel inhibitors and performed additional studies in order to have a first insight into their mechanism of action. After the identification of novel small molecules with anti-norovirus activity (part 1 of this protocol), we here describe the logical next step which entails the generation of early information of their mode of action. This information together with a continuous improvement of the potency of compounds will contribute to the optimization of a compound class towards in vivo efficacy and a successful preclinical development. SAGE Publications 2021-09-16 /pmc/articles/PMC8450984/ /pubmed/34525875 http://dx.doi.org/10.1177/20402066211025175 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Protocol
Dycke, Jana Van
Rymenants, Jasper
Neyts, Johan
Rocha-Pereira, Joana
Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Early mechanistic studies
title Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Early mechanistic studies
title_full Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Early mechanistic studies
title_fullStr Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Early mechanistic studies
title_full_unstemmed Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Early mechanistic studies
title_short Assessment of the anti-norovirus activity in cell culture using the mouse norovirus: Early mechanistic studies
title_sort assessment of the anti-norovirus activity in cell culture using the mouse norovirus: early mechanistic studies
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450984/
https://www.ncbi.nlm.nih.gov/pubmed/34525875
http://dx.doi.org/10.1177/20402066211025175
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