The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway

BACKGROUND: Acute lung injury is an important factor that leads to the death of patients with pneumonia. Previous studies have shown that nicotinamide (NAM) plays a role in reducing cell damage, so this study explored the mechanism by which NAM functions in acute lung injury. METHODS: We explored th...

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Autores principales: Zhang, Qun, Li, Junyao, Zhong, Haixia, Xu, Yanling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451170/
https://www.ncbi.nlm.nih.gov/pubmed/34544355
http://dx.doi.org/10.1186/s10020-021-00376-2
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author Zhang, Qun
Li, Junyao
Zhong, Haixia
Xu, Yanling
author_facet Zhang, Qun
Li, Junyao
Zhong, Haixia
Xu, Yanling
author_sort Zhang, Qun
collection PubMed
description BACKGROUND: Acute lung injury is an important factor that leads to the death of patients with pneumonia. Previous studies have shown that nicotinamide (NAM) plays a role in reducing cell damage, so this study explored the mechanism by which NAM functions in acute lung injury. METHODS: We explored the mechanism by which NAM affects acute lung injury in vivo and in vitro by qRT-PCR, western blotting and ELISA. RESULTS: The results showed that NAM could significantly reduce lung injury and proinflammatory mediator accumulation. Further mechanistic studies showed that NAM could significantly inhibit the MAPK and AKT/NF-κB signaling pathways. CONCLUSION: These results suggested that NAM may reduce the release of proinflammatory mediators by inhibiting the MAPK and AKT/NF-κB signaling pathways and ultimately alleviate lung injury. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s10020-021-00376-2.
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spelling pubmed-84511702021-09-20 The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway Zhang, Qun Li, Junyao Zhong, Haixia Xu, Yanling Mol Med Research Article BACKGROUND: Acute lung injury is an important factor that leads to the death of patients with pneumonia. Previous studies have shown that nicotinamide (NAM) plays a role in reducing cell damage, so this study explored the mechanism by which NAM functions in acute lung injury. METHODS: We explored the mechanism by which NAM affects acute lung injury in vivo and in vitro by qRT-PCR, western blotting and ELISA. RESULTS: The results showed that NAM could significantly reduce lung injury and proinflammatory mediator accumulation. Further mechanistic studies showed that NAM could significantly inhibit the MAPK and AKT/NF-κB signaling pathways. CONCLUSION: These results suggested that NAM may reduce the release of proinflammatory mediators by inhibiting the MAPK and AKT/NF-κB signaling pathways and ultimately alleviate lung injury. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s10020-021-00376-2. BioMed Central 2021-09-20 /pmc/articles/PMC8451170/ /pubmed/34544355 http://dx.doi.org/10.1186/s10020-021-00376-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Zhang, Qun
Li, Junyao
Zhong, Haixia
Xu, Yanling
The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway
title The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway
title_full The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway
title_fullStr The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway
title_full_unstemmed The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway
title_short The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway
title_sort mechanism of nicotinamide on reducing acute lung injury by inhibiting mapk and nf-κb signal pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451170/
https://www.ncbi.nlm.nih.gov/pubmed/34544355
http://dx.doi.org/10.1186/s10020-021-00376-2
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