Cargando…
A cross-sectional study of the nasal and fecal microbiota of sows from different health status within six commercial swine farms
BACKGROUND: Cull sows are a unique population on swine farms, often representing poor producing or compromised animals, and even though recent studies have reported that the microbiome is associated with susceptibility to diseases, the microbiome of the cull sow population has not been explored. The...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451438/ https://www.ncbi.nlm.nih.gov/pubmed/34616608 http://dx.doi.org/10.7717/peerj.12120 |
_version_ | 1784569843160186880 |
---|---|
author | Arruda, Andreia G. Deblais, Loic Hale, Vanessa L. Madden, Christopher Pairis-Garcia, Monique Srivastava, Vishal Kathayat, Dipak Kumar, Anand Rajashekara, Gireesh |
author_facet | Arruda, Andreia G. Deblais, Loic Hale, Vanessa L. Madden, Christopher Pairis-Garcia, Monique Srivastava, Vishal Kathayat, Dipak Kumar, Anand Rajashekara, Gireesh |
author_sort | Arruda, Andreia G. |
collection | PubMed |
description | BACKGROUND: Cull sows are a unique population on swine farms, often representing poor producing or compromised animals, and even though recent studies have reported that the microbiome is associated with susceptibility to diseases, the microbiome of the cull sow population has not been explored. The main objective of this study was to investigate whether there were differences in fecal and upper respiratory tract microbiota composition for groups of sows of different health status (healthy, cull, and compromised/ clinical sows) and from different farms (1 to 6). METHODS: Six swine farms were visited once. Thirty individual fecal samples and nasal swabs were obtained at each farm and pooled by five across health status and farm. Samples underwent 16S rRNA gene amplicon sequencing and nasal and fecal microbiota were analyzed using QIIME2 v.2021.4. RESULTS: Overall, the diversity of the nasal microbiota was lower than the fecal microbiota (p < 0.01). No significant differences were found in fecal or nasal alpha diversity by sow’s health status or by farm. There were significant differences in nasal microbial composition by farm and health status (PERMANOVA, p < 0.05), and in fecal microbiota by farm (PERMANOVA, p < 0.05), but not by health status. Lastly, at the L7 level, there was one differentially abundant taxa across farms for each nasal and fecal pooled samples. DISCUSSION: This study provided baseline information for nasal and fecal microbiota of sows under field conditions, and results suggest that farm of origin can affect microbial diversity and composition. Furthermore, sow’s health status may have an impact on the nasal microbiota composition. |
format | Online Article Text |
id | pubmed-8451438 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84514382021-10-05 A cross-sectional study of the nasal and fecal microbiota of sows from different health status within six commercial swine farms Arruda, Andreia G. Deblais, Loic Hale, Vanessa L. Madden, Christopher Pairis-Garcia, Monique Srivastava, Vishal Kathayat, Dipak Kumar, Anand Rajashekara, Gireesh PeerJ Agricultural Science BACKGROUND: Cull sows are a unique population on swine farms, often representing poor producing or compromised animals, and even though recent studies have reported that the microbiome is associated with susceptibility to diseases, the microbiome of the cull sow population has not been explored. The main objective of this study was to investigate whether there were differences in fecal and upper respiratory tract microbiota composition for groups of sows of different health status (healthy, cull, and compromised/ clinical sows) and from different farms (1 to 6). METHODS: Six swine farms were visited once. Thirty individual fecal samples and nasal swabs were obtained at each farm and pooled by five across health status and farm. Samples underwent 16S rRNA gene amplicon sequencing and nasal and fecal microbiota were analyzed using QIIME2 v.2021.4. RESULTS: Overall, the diversity of the nasal microbiota was lower than the fecal microbiota (p < 0.01). No significant differences were found in fecal or nasal alpha diversity by sow’s health status or by farm. There were significant differences in nasal microbial composition by farm and health status (PERMANOVA, p < 0.05), and in fecal microbiota by farm (PERMANOVA, p < 0.05), but not by health status. Lastly, at the L7 level, there was one differentially abundant taxa across farms for each nasal and fecal pooled samples. DISCUSSION: This study provided baseline information for nasal and fecal microbiota of sows under field conditions, and results suggest that farm of origin can affect microbial diversity and composition. Furthermore, sow’s health status may have an impact on the nasal microbiota composition. PeerJ Inc. 2021-09-17 /pmc/articles/PMC8451438/ /pubmed/34616608 http://dx.doi.org/10.7717/peerj.12120 Text en ©2021 Arruda et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Agricultural Science Arruda, Andreia G. Deblais, Loic Hale, Vanessa L. Madden, Christopher Pairis-Garcia, Monique Srivastava, Vishal Kathayat, Dipak Kumar, Anand Rajashekara, Gireesh A cross-sectional study of the nasal and fecal microbiota of sows from different health status within six commercial swine farms |
title | A cross-sectional study of the nasal and fecal microbiota of sows from different health status within six commercial swine farms |
title_full | A cross-sectional study of the nasal and fecal microbiota of sows from different health status within six commercial swine farms |
title_fullStr | A cross-sectional study of the nasal and fecal microbiota of sows from different health status within six commercial swine farms |
title_full_unstemmed | A cross-sectional study of the nasal and fecal microbiota of sows from different health status within six commercial swine farms |
title_short | A cross-sectional study of the nasal and fecal microbiota of sows from different health status within six commercial swine farms |
title_sort | cross-sectional study of the nasal and fecal microbiota of sows from different health status within six commercial swine farms |
topic | Agricultural Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451438/ https://www.ncbi.nlm.nih.gov/pubmed/34616608 http://dx.doi.org/10.7717/peerj.12120 |
work_keys_str_mv | AT arrudaandreiag acrosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT deblaisloic acrosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT halevanessal acrosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT maddenchristopher acrosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT pairisgarciamonique acrosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT srivastavavishal acrosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT kathayatdipak acrosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT kumaranand acrosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT rajashekaragireesh acrosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT arrudaandreiag crosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT deblaisloic crosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT halevanessal crosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT maddenchristopher crosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT pairisgarciamonique crosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT srivastavavishal crosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT kathayatdipak crosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT kumaranand crosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms AT rajashekaragireesh crosssectionalstudyofthenasalandfecalmicrobiotaofsowsfromdifferenthealthstatuswithinsixcommercialswinefarms |